scholarly journals A purified human platelet pellet lysate rich in neurotrophic factors and antioxidants repairs and protects corneal endothelial cells from oxidative stress

2021 ◽  
Vol 142 ◽  
pp. 112046
Author(s):  
Rifa Widyaningrum ◽  
Thierry Burnouf ◽  
Ouada Nebie ◽  
Liling Delila ◽  
Tsung-Jen Wang
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Neurotrophic Factors ◽  
Human Platelet ◽  
Corneal Endothelial Cells ◽  
Platelet Pellet

Oxidative Stress Induces a Breakdown of the Cytoskeleton and Tight Junctions of the Corneal Endothelial Cells

2021 ◽  
Author(s):  
Anupama Chalimeswamy ◽  
Marasarakottige Yogananda Thanuja ◽  
Sudhir H. Ranganath ◽  
Kaveet Pandya ◽  
Uday B. Kompella ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Tight Junctions ◽  
Corneal Endothelial Cells

scholarly journals The neuroprotective activity of heat-treated human platelet lysate biomaterials manufactured from outdated pathogen-reduced (amotosalen/UVA) platelet concentrates

2019 ◽  
Vol 26 (1) ◽  
Author(s):  
Ouada Nebie ◽  
David Devos ◽  
Valérie Vingtdeux ◽  
Lassina Barro ◽  
Jean-Christophe Devedjian ◽  
...  
Keyword(s):  
Neurotrophic Factors ◽  
Industrial Development ◽  
Human Platelet ◽  
Neuron Specific Enolase ◽  
Source Material ◽  
Neuroprotective Activity ◽  
Primary Neurons ◽  
Platelet Concentrates ◽  
Heat Treated ◽  
Platelet Pellet

Abstract Background Effective neurorestorative therapies of neurodegenerative diseases must be developed. There is increasing interest in using human platelet lysates, rich in neurotrophic factors, as novel disease-modifying strategy of neurodegeneration. To ensure virus safety, pathogen reduction treatments should be incorporated in the preparation process of the platelet concentrates used as source material. We therefore investigated whether platelet concentrates (PC) pathogen-inactivated using a licensed photo-inactivation treatment combining photosensitive psoralen (amotosalen) and UVA irradiation (Intercept) can serve as source material to prepare platelet lysates with preserved neuroprotective activity in Parkinson’s disease models. Methods Intercept treated-PCs were centrifuged, when reaching expiry day (7 days after collection), to remove plasma and platelet additive solution. The platelet pellet was re-suspended and concentrated in phosphate buffer saline, subjected to 3 freeze-thaw cycles (− 80 °C/37 °C) then centrifuged to remove cell debris. The supernatant was recovered and further purified, or not, by heat-treatment as in our previous investigations. The content in proteins and neurotrophic factors was determined and the toxicity and neuroprotective activity of the platelet lysates towards LUHMES cells or primary cortical/hippocampal neurons were assessed using ELISA, flow cytometry, cell viability and cytotoxicity assays and proteins analysis by Western blot. Results Platelet lysates contained the expected level of total proteins (ca. 7–14 mg/mL) and neurotrophic factors. Virally inactivated and heat-treated platelet lysates did not exert detectable toxic effects on neither Lund human mesencephalic dopaminergic LUHMES cell line nor primary neurons. When used at doses of 5 and 0.5%, they enhanced the expression of tyrosine hydroxylase and neuron-specific enolase in LUHMES cells and did not significantly impact synaptic protein expression in primary neurons, respectively. Furthermore, virally-inactivated platelet lysates tested were found to exert very strong neuroprotection effects on both LUHMES and primary neurons exposed to erastin, an inducer of ferroptosis cell death. Conclusion Outdated Intercept pathogen-reduced platelet concentrates can be used to prepare safe and highly neuroprotective human heat-treated platelet pellet lysates. These data open reassuring perspectives in the possibility to develop an effective biotherapy using virally-inactivated platelet lysates rich in functional neurotrophins for neuroregenerative medicine, and for further bio-industrial development. However, the data should be confirmed in animal models. Graphical abstract

Effect of Cysteamine on Oxidative Stress-induced Cell Death of Human Corneal Endothelial Cells

2011 ◽  
Vol 36 (10) ◽  
pp. 910-917 ◽  
Author(s):  
Young Joo Shin ◽  
Jong Mo Seo ◽  
Tae Young Chung ◽  
Joon Young Hyon ◽  
Won Ryang Wee
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Cell Death ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells

scholarly journals Screening and Characterization of Drugs That Protect Corneal Endothelial Cells Against Unfolded Protein Response and Oxidative Stress

2017 ◽  
Vol 58 (2) ◽  
pp. 892 ◽  
Author(s):  
Eun Chul Kim ◽  
Tetsuya Toyono ◽  
Cynthia A. Berlinicke ◽  
Donald J. Zack ◽  
Ula Jurkunas ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Unfolded Protein Response ◽  
Corneal Endothelial Cells ◽  
Unfolded Protein ◽  
Protein Response ◽  
And Oxidative Stress

scholarly journals Potential Effect of Human Platelet Lysate on in vitro Expansion of Human Corneal Endothelial Cells Compared with Y-27632 ROCK Inhibitor

2021 ◽  
Author(s):  
Mohammad Amir Mishan ◽  
Sahar Balagholi ◽  
Tahereh Chamani ◽  
Sepehr Feizi ◽  
Zahra-Soheila Soheili ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Corneal Endothelial Cells ◽  
Rock Inhibitor ◽  
Treatment Groups ◽  
Medium Supplement ◽  
Proliferative Effect ◽  
Human Platelet Lysate

Purpose: Corneal endothelial cell (CEC) therapy can be used as a promising therapeutic option for patients with various corneal endothelial dysfunctions. In this study, we compared the proliferative effect of human platelet lysate (HPL), as a xeno-free medium supplement, with Y-27632 Rho/rho-associated protein kinase (ROCK) inhibitor, as a wellknown proliferative and adhesive agent for CECs, and fetal bovine serum (FBS) as the control, in the culture medium of human corneal endothelial cells (HCECs). Methods: We isolated HCECs from human donors and treated the cells as three different treatment groups including 20% HPL only, 10 μM Y-27632 ROCK inhibitor, combination of 20% HPL and 10 μM Y-27632 ROCK inhibitor, and 20% FBS as the control group. ELISA cell proliferation assay and cell counting was performed on the treated cells. Finally, HCECs were characterized by morphology and immunocytochemistry (ICC). Results: There was no significant proliferative effect of HPL on cell proliferation compared with the cells treated with Y-27632 ROCK inhibitor or the combination of HPL and Y-27632 ROCK inhibitor, but all the respected treatments had significant inducible effect on cell proliferation as compared with FBS-treated cells. The cells grown in all three treatment groups exhibited CEC morphology. Also, there was a higher expression of Na+/K+-ATPase and ZO-1, as CEC characteristic markers, in the culture of HCECs treated with HPL as compared with FBS. Conclusion: HPL offers a xeno−free and affordable medium supplement for CEC expansion that can be used in clinical applications.

Potential of a novel scaffold composed of human platelet lysate and fibrin for human corneal endothelial cells

2021 ◽  
Author(s):  
Mohammad Amir Mishan ◽  
Sahar Balagholi ◽  
Tahereh Chamani ◽  
Sepehr Feizi ◽  
Zahra-Soheila Soheili ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells ◽  
Human Platelet Lysate ◽  
Novel Scaffold

scholarly journals Regulation of Oxidative Stress in Corneal Endothelial Cells by Prdx6

Antioxidants ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 180 ◽  
Author(s):  
Matthew Lovatt ◽  
Khadijah Adnan ◽  
Gary Peh ◽  
Jodhbir Mehta
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Corneal Endothelium ◽  
Corneal Dystrophy ◽  
The Body ◽  
Corneal Endothelial Cells ◽  
Functional Studies ◽  
Age Dependent ◽  
Cellular Integrity

The inner layer of the cornea, the corneal endothelium, is post-mitotic and unable to regenerate if damaged. The corneal endothelium is one of the most transplanted tissues in the body. Fuchs’ endothelial corneal dystrophy (FECD) is the leading indication for corneal endothelial transplantation. FECD is thought to be an age-dependent disorder, with a major component related to oxidative stress. Prdx6 is an antioxidant with particular affinity for repairing peroxidised cell membranes. To address the role of Prdx6 in corneal endothelial cells, we used a combination of biochemical and functional studies. Our data reveal that Prdx6 is expressed at unusually high levels at the plasma membrane of corneal endothelial cells. RNAi-mediated knockdown of Prdx6 revealed a role for Prdx6 in lipid peroxidation. Furthermore, following induction of oxidative stress with menadione, Prdx6-deficient cells had defective mitochondrial membrane potential and were more sensitive to cell death. These data reveal that Prdx6 is compartmentalised in corneal endothelial cells and has multiple functions to preserve cellular integrity.

Evaluation of phacoemulsification-induced oxidative stress and damage of cultured human corneal endothelial cells in different solutions using redox fluorometry microscopy

2010 ◽  
Vol 88 (8) ◽  
pp. e323-e327 ◽  
Author(s):  
Yutaro Nishi ◽  
Christoph Engler ◽  
Dae Ro Na ◽  
Renata T. Kashiwabuchi ◽  
Young Joo Shin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells
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