Fructans from Codonopsis pilosula Roots

2021 ◽  
Author(s):  
Jiankuan Li ◽  
Yan Wang ◽  
Junqing Zhang ◽  
Yune Bai ◽  
Jianping Gao
Keyword(s):  
Codonopsis Pilosula

scholarly journals Response of Bioactive Metabolite and Biosynthesis Related Genes to Methyl Jasmonate Elicitation in Codonopsis pilosula

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 533 ◽  
Author(s):  
Jiao-jiao Ji ◽  
Qi Feng ◽  
Hai-feng Sun ◽  
Xue-jun Zhang ◽  
Xiao-xiao Li ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Adventitious Roots ◽  
Multiple Shoots ◽  
Bioactive Metabolites ◽  
Bioactive Metabolite ◽  
Codonopsis Pilosula ◽  
Meja Treatment ◽  
Metabolite Accumulation ◽  
Culture Growth ◽  
Sucrose Level

Bioactive metabolites in Codonopsis pilosula are of particular interest as an immunostimulant. Methyl jasmonate (MeJA) plays an important role in the elicitation of metabolite biosynthesis. Here, we explored the response of metabolites to MeJA elicitation in C. pilosula adventitious roots and multiple shoots. The results showed that the biomass, polysaccharide, and lobetyolin content of adventitious roots exhibited the highest increases with 100 µmol·L−1 MeJA at the 16th day of subculture, whereas the atractylenolide III (a terpenoid) content increased extremely with 50 µmol·L−1 MeJA treatment at the 7th day of subculture. In addition, the biomass and lobetyolin content significantly increased at the 4th day after treatment. Similarly, the polysaccharide and lobetyolin content increased in multiple shoots. Further identification of different metabolites responding to MeJA by 1H-NMR showed an extremely significant increase of the lobetyolinin level, which coincided with lobetyolin. Accordingly, the precursor, fatty acids, showed a highly significant decrease in their levels. Furthermore, a significant increase in β-d-fructose-butanol glycoside was detected, which was accompanied by a decrease in the sucrose level. Accordingly, the enzyme genes responsible for terpenoid and carbohydrate biosynthesis, CpUGPase, and CpPMK, were up regulated. In conclusion, MeJA promoted culture growth and accelerated bioactive metabolite accumulation by regulating the expression of the metabolite biosynthesis related genes, CpUGPase and CpPMK in C. pilosula.

Validation of Reliable Reference Genes for Accurate Normalization in RT-qPCR Analysis of Codonopsis pilosula

2017 ◽  
Vol 9 (3) ◽  
pp. 226-235 ◽  
Author(s):  
Ling-ya Cao ◽  
Xiao-xiao Li ◽  
Dong Wang ◽  
Hai-feng Sun ◽  
Jian-ping Gao
Keyword(s):  
Reference Genes ◽  
Codonopsis Pilosula ◽  
Qpcr Analysis ◽  
Accurate Normalization

scholarly journals Direct multiple shoot induction and plant regeneration from dormant buds of Codonopsis pilosula (Franch.) Nannf.

2011 ◽  
Vol 10 (51) ◽  
pp. 10509-10515 ◽  
Author(s):  
H Zhang Y ◽  
F Gao S ◽  
Du T ◽  
G Chen H ◽  
H Z Wang ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Multiple Shoot ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Codonopsis Pilosula

scholarly journals Codonopsis pilosula Polysaccharide Attenuates Tau Hyperphosphorylation and Cognitive Impairments in hTau Infected Mice

2018 ◽  
Vol 11 ◽  
Author(s):  
Qing Zhang ◽  
Yiyuan Xia ◽  
Hongbin Luo ◽  
Sheng Huang ◽  
Yongjun Wang ◽  
...  
Keyword(s):  
Cognitive Impairments ◽  
Tau Hyperphosphorylation ◽  
Codonopsis Pilosula

scholarly journals Codonopsis pilosula Extract Reverse Glucose Tolerance Impairment-Induced Sperm Dysfunction.

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 518-518
Author(s):  
Chia-Ming Hsu ◽  
Hsiu Chuan Chang ◽  
Chih-Chien Chen ◽  
Shiow-Chwen Tsai
Keyword(s):  
Glucose Tolerance ◽  
Codonopsis Pilosula ◽  
Sperm Dysfunction

scholarly journals Establishment of in vitro culture system for Codonopsis pilosula transgenic hairy roots

3 Biotech ◽  
2020 ◽  
Vol 10 (3) ◽  
Author(s):  
Jing Yang ◽  
Xiaozeng Yang ◽  
Bin Li ◽  
Xiayang Lu ◽  
Jiefang Kang ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Hairy Roots ◽  
Culture System ◽  
Codonopsis Pilosula ◽  
In Vitro Culture System ◽  
Transgenic Hairy Roots

Effect of Codonopsis pilosula on Immune and Digestion in Sea Cucumber, Apostichopus japonicus

2018 ◽  
Vol 44 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Y. Fan ◽  
J. Diao ◽  
X. Q. Yu ◽  
L. Li ◽  
E. F. Liu ◽  
...  
Keyword(s):  
Sea Cucumber ◽  
Apostichopus Japonicus ◽  
Codonopsis Pilosula

Antioxidant Effects of Codonopsis pilosula Reverse Glucose Tolerance Impairment-Induced Testicular Dysfunction.

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 519-519
Author(s):  
Kuan-Hua Chiu ◽  
Hsiu Chuan Chang ◽  
Chih-Chien Chen ◽  
Shiow-Chwen Tsai
Keyword(s):  
Glucose Tolerance ◽  
Codonopsis Pilosula ◽  
Testicular Dysfunction ◽  
Antioxidant Effects
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