scholarly journals Evaluating osteochondral defect repair potential of autologous rabbit bone marrow cells on type II collagen scaffold

2010 ◽  
Vol 63 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Wei-Chuan Chen ◽  
Chao-Ling Yao ◽  
Yu-Hong Wei ◽  
I-Ming Chu
Keyword(s):  
Bone Marrow ◽  
Osteochondral Defect ◽  
Bone Marrow Cells ◽  
Collagen Scaffold ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Defect Repair ◽  
Rabbit Bone ◽  
Marrow Cells

Biosynthesis of cholinesterase in rabbit bone marrow cells in culture

1974 ◽  
Vol 23 (15) ◽  
pp. 2155-2163 ◽  
Author(s):  
Larrel W. Harris ◽  
Vincent F. Garry ◽  
Robert D. Moore
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Cells In Culture ◽  
Rabbit Bone ◽  
Marrow Cells

scholarly journals CELLS INVOLVED IN THE IMMUNE RESPONSE

1969 ◽  
Vol 129 (6) ◽  
pp. 1261-1273 ◽  
Author(s):  
M. Richter ◽  
N. I. Abdou
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Plaque Formation ◽  
Lymphoid Cells ◽  
Spleen Cells ◽  
Normal Bone ◽  
Large Numbers ◽  
Recipient Animal ◽  
Rabbit Bone ◽  
Marrow Cells

Bone marrow cells obtained from rabbits of one allotype were injected into irradiated rabbits of a different allotype. The recipients were also injected with sheep red blood cells, and their spleen cells were tested for plaque-forming capacity 7 days later. Spleen cells of all recipients gave large numbers of plaques as did spleen cells incubated with antiserum, directed toward donor allotype. However, incubation of the recipient spleen cells with antiserum directed toward recipient allotype completely suppressed plaque formation. These results demonstrate that antibody-formation in irradiated recipients of transferred lymphoid cells is a property of the recipient animal and that the antibody-forming cell is relatively irradiation-resistant. It was also demonstrated that only viable normal bone marrow cells are capable of transferring antibody-forming capacity to irradiated recipient rabbits. Neither sonicates nor heat-killed preparations of normal rabbit bone marrow cells possessed this capacity.

scholarly journals Histochemical Demonstration of Phosphorylase in Blood and Bone Marrow Cells

Blood ◽  
1956 ◽  
Vol 11 (4) ◽  
pp. 375-379 ◽  
Author(s):  
TADAO TAKEUCHI ◽  
KENJI KINOSHITA
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Bone Marrow Cells ◽  
Histochemical Demonstration ◽  
Staining Reaction ◽  
Phosphorylase Activity ◽  
Iodine Staining ◽  
Rabbit Bone ◽  
Marrow Cells

Abstract Phosphorylase activity has been demonstrated histochemically in blood and bone marrow cells. The polysaccharide newly synthesized from glucose-1-phosphate by the enzyme of these cells gave a blue or violet blue iodine staining reaction. The reaction occurred in cytoplasm and the nuclei did not stain. This was shown in the neutrophilic, eosinophilic or pseudoeosinophilic leukocytes of man, dog, rabbit and pigeon peripheral blood. It was likewise demonstrated in the bicolored leukocytes of snake, fish and frog. The reaction also appeared in the neutrophilic, pseudoeosinophilic or eosinophilic leukocytes, metamyelocytes, myelocytes and promyelocytes of human and rabbit bone marrow smears.

scholarly journals Cell-type-related segregation of surface galactosyl-containing components at an early developmental stage in hemopoietic bone marrow cells in the rabbit.

1983 ◽  
Vol 96 (1) ◽  
pp. 184-190 ◽  
Author(s):  
E Skutelsky ◽  
E A Bayer
Keyword(s):  
Bone Marrow ◽  
Cell Surface ◽  
Bone Marrow Cells ◽  
Galactose Oxidase ◽  
Early Developmental Stage ◽  
Neuraminidase Treatment ◽  
Rabbit Bone ◽  
Erythroid Development ◽  
Galactosyl Residues ◽  
Marrow Cells

The avidin-biotin complex was used for the selective ultrastructural labeling of terminal cell surface galactosyl residues. Rabbit bone marrow cells were treated with the enzyme galactose oxidase in the presence of biotin hydrazide. Subsequent treatment with ferritin-avidin conjugates enabled the electron microscopic visualization of terminal membrane-based galactose and/or N-acetylgalactosamine on these cells. All stages of erythroid development were characterized by high levels of exposed cell surface galactose, whereas all leukoid cells in the same preparations were virtually unlabeled by the above method. Modulations in the distribution of these surface determinants during differentiation and maturation of rabbit erythroid cells were found to concur in inverse fashion with respect to that of terminal sialic acids. Neuraminidase treatment, before the above labeling procedure, resulted in the exposure of additional galactosyl residues on the surface of all bone marrow cell types. The results indicate that a galactose-bearing glycoconjugate(s) may comprise an erythroid-specific membrane constituent of rabbit bone marrow cells. The high density of galactose on the surface of even the earliest erythroid precursors may eventually enable the identification and isolation of a stem cell, which already contains the erythroid-specific galactoconjugate(s). The results suggest that variations in the spectrum of cell surface carbohydrates may serve as recognition signals in the complex set of intercellular interactions which occur during the development and maturation of the erythrocyte. The occurrence of similar but species-specific variations in the complement of surface heterosaccharides during erythroid development of humans and other mammals supports this contention.

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