Effects of graft and aphid transmission on the genetic diversity and population structure of Turkish citrus tristeza virus isolates

2021 ◽  
Author(s):  
Savaş Korkmaz ◽  
Ali Karanfil ◽  
Serdar Satar ◽  
Tugba Uslu ◽  
Namık Kemal Koç ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Citrus Tristeza Virus ◽  
Aphid Transmission ◽  
Tristeza Virus ◽  
Virus Isolates ◽  
Citrus Tristeza

scholarly journals Molecular Analyses of Citrus tristeza virus Subisolates Separated by Aphid Transmission

Plant Disease ◽  
2003 ◽  
Vol 87 (4) ◽  
pp. 397-401 ◽  
Author(s):  
R. H. Brlansky ◽  
V. D. Damsteegt ◽  
D. S. Howd ◽  
A. Roy
Keyword(s):  
Monoclonal Antibody ◽  
Citrus Tristeza Virus ◽  
Aphid Transmission ◽  
Variable Regions ◽  
Molecular Analyses ◽  
Field Isolates ◽  
Molecular Profiles ◽  
Tristeza Virus ◽  
Virus Isolates ◽  
Citrus Tristeza

Citrus tristeza virus (CTV) exists in field isolates as a complex of virus isolates. This complex may contain both mild and severe CTV. Using single and multiple aphid transmissions, subiso-lates of the various field isolates were separated. Some CTV isolates that tested negative with the monoclonal antibody MCA13 consisted of MCA13-positive subisolates. Using primers to specific and variable regions of the CTV genome, molecular profiles of the isolates and subisolates were generated and compared. The profiles of the subisolates sometimes were very different from the parent field isolates from which they were transmitted.

scholarly journals Aphid Transmission Alters the Genomic and Defective RNA Populations of Citrus tristeza virus Isolates

2000 ◽  
Vol 90 (2) ◽  
pp. 134-138 ◽  
Author(s):  
M. R. Albiach-Martí ◽  
J. Guerri ◽  
A. Hermoso de Mendoza ◽  
F. Laigret ◽  
J. F. Ballester-Olmos ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Blot Hybridization ◽  
Aphis Gossypii ◽  
Aphid Transmission ◽  
Northern Blot Hybridization ◽  
Dot Blot ◽  
Defective Rnas ◽  
Tristeza Virus ◽  
Virus Isolates ◽  
Citrus Tristeza

A total of 14 Spanish isolates of Citrus tristeza virus (CTV) and 1 isolate from Japan were transmitted by Aphis gossypii, and the subisolates obtained were compared with the source isolates for symptom expression and double-stranded RNA (dsRNA) pattern. Of the 14 Spanish isolates, 9 showed altered dsRNA patterns after aphid transmission but only minor variations in the intensity of symptoms induced on Mexican lime. Northern blot hybridization with complementary DNA (cDNA) probes corresponding to both the 5′ and the 3′ termini of the CTV genomic RNA (gRNA) showed that the dsRNA bands that could be used to discriminate between the dsRNA pattern of the source and the aphid-transmitted isolates were the replicative forms of defective RNAs (D-RNAs). Conversely, the Japanese isolate and two subisolates obtained from it by aphid transmission had the same dsRNA pattern, but one of the subisolates induced milder symptoms in several hosts. Dot-blot hybridization with cDNA probes representing several regions of the gRNA showed that most of the aphid-transmitted isolates differed from the corresponding source isolate by their hybridization pattern. Our results indicate that aphid transmission often sorts the populations of gRNA variants and D-RNAs present in CTV isolates.

Genetic diversity and evolution of two capsid protein genes of citrus tristeza virus isolates from China

2014 ◽  
Vol 160 (3) ◽  
pp. 787-794 ◽  
Author(s):  
Guan-Wei Wu ◽  
Min Tang ◽  
Guo-Ping Wang ◽  
Feng-Yin Jin ◽  
Zuo-Kun Yang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Capsid Protein ◽  
Citrus Tristeza Virus ◽  
Tristeza Virus ◽  
Virus Isolates ◽  
Citrus Tristeza

Exploring the genetic diversity and recombination analysis of Citrus tristeza virus isolates prevalent in Northeast India

2020 ◽  
Vol 73 (1) ◽  
pp. 145-153
Author(s):  
Trishnamoni Gautom ◽  
Dibya Jyoti Hazarika ◽  
Gunajit Goswami ◽  
Madhumita Barooah ◽  
Mohan Chandra Kalita ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Citrus Tristeza Virus ◽  
Northeast India ◽  
Recombination Analysis ◽  
Tristeza Virus ◽  
Virus Isolates ◽  
Citrus Tristeza

scholarly journals Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Gözde Erkiş-Güngör ◽  
Bayram Çevik
Keyword(s):  
Phylogenetic Analysis ◽  
Citrus Tristeza Virus ◽  
Molecular Characteristics ◽  
Cloning And Sequencing ◽  
Phylogenetic Groups ◽  
The 1960S ◽  
Tristeza Virus ◽  
Virus Isolates ◽  
Stem Pitting ◽  
Citrus Tristeza

The presence of Citrus tristeza virus (CTV) in Turkey has been known since the 1960s and the virus was detected in all citrus growing regions of the country. Even though serological and biological characteristics of CTV have been studied since the 1980s, molecular characteristics of CTV isolates have not been studied to date in Turkey. In this study, molecular characteristics of 15 CTV isolates collected from different citrus growing regions of Turkey were determined by amplification, cloning, and sequencing of their major coat protein (CP) genes. The sequence analysis showed that the CP genes were highly conserved among Turkish isolates. However, isolates from different regions showed more genetic variation than isolates from the same region. Turkish isolates were clustered into three phylogenetic groups showing no association with geographical origins, host, or symptoms induced in indicator plants. Phylogenetic analysis of Turkish isolates with isolates from different citrus growing regions of the world including well-characterized type isolates of previously established strain specific groups revealed that some Turkish isolates were closely related to severe quick decline or stem pitting isolates. The results demonstrated that although CTV isolates from Turkey are considered biologically mild, majority of them contain severe components potentially causing quick decline or stem pitting.

scholarly journals Stem-Pitting Citrus tristeza virus Predominantly Transmitted by the Brown Citrus Aphid from Mixed Infections Containing Non-Stem-Pitting and Stem-Pitting Isolates

Plant Disease ◽  
2011 ◽  
Vol 95 (8) ◽  
pp. 913-920 ◽  
Author(s):  
R. H. Brlansky ◽  
Avijit Roy ◽  
V. D. Damsteegt
Keyword(s):  
Citrus Tristeza Virus ◽  
Fruit Size ◽  
Haplotype Diversity ◽  
Aphid Transmission ◽  
Single Strand Conformation Polymorphism ◽  
Mixed Infections ◽  
Tristeza Virus ◽  
Stem Pitting ◽  
Citrus Spp ◽  
Citrus Tristeza

Citrus tristeza virus (CTV) is a phloem-limited Closterovirus that produces a variety of symptoms in various Citrus spp. One of these symptoms is stem pitting (SP). SP does not occur in all Citrus spp. but when it does it may cause low tree vigor, decline, and an economic reduction in fruit size and yield. Historically, the first appearance of CTV-SP in a citrus area often occurs after the introduction of the most efficient CTV vector, the brown citrus aphid (BCA), Toxoptera citricida. Hypotheses for this association range from the introduction of these strains in new planting materials to the increased ability of BCA to transmit SP strains from existing CTV sources. It is known that CTV often exists as a complex of isolates or subisolates. Single and multiple BCA transmissions have been used to separate different genotypes or strains of CTV from mixed CTV infected plants. This study was initiated to determine what the BCA transmits when an exotic severe SP CTV isolate B12 from Brazil or B408 from Dominican Republic are mixed with a non-SP (NSP) isolate, FS627 from Florida. Biological and molecular data was generated from grafted mixtures of these isolates and their aphid-transmitted subisolates. Single-strand conformation polymorphism patterns of the 5′ terminal region of open reading frame (ORF) 1a, the overlapping region of ORF1b and ORF2, and the major coat protein gene region of NSP and SP CTV-grafted plants remained unchanged but the patterns of doubly inoculated plants varied. The haplotype diversity within SP isolates B12, B408, and mixtures of NSP and SP isolates (FS627/B12 and FS627/B408) and aphid-transmitted subisolates from doubly inoculated plants was determined by analysis of the haplotype nucleotide sequences. Aphid transmission experiments, symptoms, and molecular analyses showed that SP-CTV was more frequently transmitted with or without NSP-CTV from mixed infections.

scholarly journals Citrus Tristeza Virus Isolates of the Same Genotype Differ in Stem Pitting Severity in Grapefruit

Plant Disease ◽  
2020 ◽  
Vol 104 (9) ◽  
pp. 2362-2368
Author(s):  
Glynnis Cook ◽  
Beatrix Coetzee ◽  
Rachelle Bester ◽  
Johannes H. J. Breytenbach ◽  
Chanel Steyn ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Aphid Transmission ◽  
Common Source ◽  
Citrus Paradisi ◽  
Full Genome ◽  
Full Genome Sequencing ◽  
Reading Frame ◽  
Tristeza Virus ◽  
Stem Pitting ◽  
Citrus Tristeza

Two isolates of the T68 genotype of citrus tristeza virus (CTV) were derived from a common source, GFMS12, by single aphid transmission. These isolates, named GFMS12-8 and GFMS12-1.3, induced stem pitting with differing severity in ‘Duncan’ grapefruit (Citrus × paradisi [Macfad.]). Full-genome sequencing of these isolates showed only minor nucleotide sequence differences totaling 45 polymorphisms. Numerous nucleotide changes, in relatively close proximity, were detected in the p33 open reading frame (ORF) and the leader protease domains of ORF1a. This is the first report of full-genome characterization of CTV isolates of a single genotype, derived from the same source, but showing differences in pathogenicity. The results demonstrate the development of intragenotype heterogeneity known to occur with single-stranded RNA viruses. Identification of genetic variability between isolates showing different pathogenicity will enable interrogation of specific genome regions for potential stem pitting determinants.

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