Establishing influence of morphological aspects on microclimatic conditions through GIS-assisted mathematical modeling and field observations

Abstract The article discusses a numerical model for assessing the depth for the passive method of induced polarization based on previously obtained experimental data at the Samson field (Republic of Khakassia). The model is based on the mathematical model of Komarov, who derived equations for the anomalous polarizability of a sphere observed on the Earth’s day surface, the proposed model for estimating the depth of the anomaly depends on the size of the proposed field and the induced polarization coefficient observed on the surface. In the course of the numerical assessment, it was shown that there is a convergence of data from field observations, exploration drilling and mathematical modeling.

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Modelling of technological process of joining steel ropes with the use of powder antifriction material and a conductor

Journal of Physics Conference Series ◽

10.1088/1742-6596/2131/4/042060 ◽

2021 ◽

Vol 2131 (4) ◽

pp. 042060

Author(s):

E V Marchenko ◽

A A Korotky ◽

S I Popov ◽

Ju V Marchenko ◽

E E Kosenko

Keyword(s):

Mathematical Modeling ◽

Experimental Studies ◽

Antifriction Material ◽

Working Environment ◽

Labor Process ◽

Structural Defects ◽

Field Observations ◽

Steel Rope ◽

Joint Section

Abstract When determining the causes of structural defects of a steel rope, the technology of carrying out work on splicing by a team of specialists was studied in detail. Field observations and experimental studies, as well as mathematical modeling of the process of structural defects formation in the rope joint section, allowed to establish that the cause is the imperfection of the existing technology for performing splice work [1-5]. The existing technology directly depends on the experience (professionalism) of a splicing team and provides for the work on splicing, as a solution to a new problem for every hawser, expressed in the design and calculation of the main parameters, according to the established standards of the rope manufacturer. It is noted that this labor function negatively affects a person, causing dangerous and harmful factors of the working environment, such as: severity and intensity of the labor process. Under the influence of dangerous and harmful factors, the specialist is subject to: static and dynamic overload, mental overstrain, neuropsychiatric overload, which negatively affect a person and, as a result, affect the quality of the work performed as a whole. The total accumulation of dangerous factors in the course of work leads to the decrease in the quality of the obtained results and deviation from the normalized indicators, which is unacceptable and leads to the formation of structural defects of a steel rope.

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Electron Microscopy of Mycoplasma Pneumoniae

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065341 ◽

1971 ◽

Vol 29 ◽

pp. 258-259 ◽

Cited By ~ 1

Author(s):

E. S. Boatman ◽

G. E. Kenny

Keyword(s):

Electron Microscopy ◽

Light Microscopy ◽

Growth Phase ◽

Hela Cells ◽

Sulfonic Acid ◽

Gamma Globulin ◽

Horse Serum ◽

Morphological Aspects ◽

The Family

Information concerning the morphology and replication of organism of the family Mycoplasmataceae remains, despite over 70 years of study, highly controversial. Due to their small size observations by light microscopy have not been rewarding. Furthermore, not only are these organisms extremely pleomorphic but their morphology also changes according to growth phase. This study deals with the morphological aspects of M. pneumoniae strain 3546 in relation to growth, interaction with HeLa cells and possible mechanisms of replication.The organisms were grown aerobically at 37°C in a soy peptone yeast dialysate medium supplemented with 12% gamma-globulin free horse serum. The medium was buffered at pH 7.3 with TES [N-tris (hyroxymethyl) methyl-2-aminoethane sulfonic acid] at 10mM concentration. The inoculum, an actively growing culture, was filtered through a 0.5 μm polycarbonate “nuclepore” filter to prevent transfer of all but the smallest aggregates. Growth was assessed at specific periods by colony counts and 800 ml samples of organisms were fixed in situ with 2.5% glutaraldehyde for 3 hrs. at 4°C. Washed cells for sectioning were post-fixed in 0.8% OSO4 in veronal-acetate buffer pH 6.1 for 1 hr. at 21°C. HeLa cells were infected with a filtered inoculum of M. pneumoniae and incubated for 9 days in Leighton tubes with coverslips. The cells were then removed and processed for electron microscopy.

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