A practical method for almond cultivar identification and parental analysis using simple sequence repeat markers

Nine simple-sequence-repeat (SSR) primer pairs were assayed in 35 Spanish and Italian olive cultivars of commercial interest. All microsatellites were polymorphic, showing 5 to 13 alleles per locus (7.5 alleles per locus on average). The frequency of each alleles was generally low, with most of the alleles present at one or two cultivars. Heterozigosity ranged from 0.15 to 0.95; the discrimination power (PD) ranged from 0.30 to 0.93 (mean 0.79). The set of microsatellites analyzed discriminated all cultivars investigated. The combination of only three SSR primer pairs—UDO99-009+UDO99-043+UDO99-14—made possible the identification of all cultivars included in the study. Cluster analysis did not find differences between Spanish and Italian cultivars, but most of the cultivars from southern and central Spain grouped together. Hence, microsatellites markers are recommended for olive fingerprinting to generate a database for olive cultivar identification.

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Molecular Identification of Commercial Apple Cultivars with Microsatellite Markers

HortScience ◽

10.21273/hortsci.40.7.1974 ◽

2005 ◽

Vol 40 (7) ◽

pp. 1974-1977 ◽

Cited By ~ 35

Author(s):

Zsolt Galli ◽

Gábor Halász ◽

Erzsébet Kiss ◽

László Heszky ◽

Judit Dobránszki

Keyword(s):

Molecular Identification ◽

Polymorphism Information Content ◽

Simple Sequence Repeat ◽

Cultivar Identification ◽

Cumulative Probability ◽

Sequence Repeat ◽

Simple Sequence Repeat Markers ◽

Ssr Loci ◽

Apple Cultivars ◽

Simple Sequence

A collection of 66 commercial apple (Malus ×domestica Borkh.) cultivars was screened with six previously described SSR (Simple Sequence Repeat) markers for molecular identification. In total, 55 polymorphic alleles were detected at the 6 SSR loci (average 9.2 alleles per locus) and the polymorphism information content (PIC) averaged 0.72. Successful differentiation of all apple genotypes except for somatic mutants was accomplished by using only four (CH03g07, CH04e03, CH05d11, and CH05e03) SSR markers. Sport mutants proved to be indistinguishable from each other and their progenitors. The cumulative probability of obtaining an identical SSR profile for two randomly chosen apple genotypes was 1.79 × 10–4, which confirms the high potential of simple sequence repeats (SSRs) for cultivar identification.

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Optimization of the choice of molecular markers for identification of commercially used rice varieties in India using rapid DNA extraction protocol

Journal of Applied and Natural Science ◽

10.31018/jans.v8i2.916 ◽

2016 ◽

Vol 8 (2) ◽

pp. 1028-1034

Author(s):

Pravas Ranjan Kole ◽

Rajeev Singh Rana ◽

Kangila Venkataramana Bhat

Keyword(s):

Molecular Marker ◽

Simple Sequence Repeat ◽

Cultivar Identification ◽

Genetic Identification ◽

Sequence Repeat ◽

Genetic Purity ◽

Traditional Practice ◽

Rice Varieties ◽

Simple Sequence Repeat Markers ◽

Simple Sequence

The present investigating aimed at the development of molecular marker for cultivar identification and genetic purity assessment. A total of four SSR markers and six SRAP primer were developed for the identification of sixteen different commercial varieties of rice. Traditional practice like grow-out-test based on morphological traits is time consuming and sometimes environmentally influenced. After development of molecular marker, it is using as an alternative to grow â€“out â€“test because of its rapid, accurate detection. We have assessed the potential of simple sequence repeat and sequence-related amplified polymorphism markers in distinguishing rice varieties and four simple sequence repeat markers namely CT-14, CT-25 CT XY-1 and ATC-3 and six sequence-related amplified polymorphism markers primers could be clearly distinguished sixteen commercially cultivar rice varieties. In addition to single markers, itâ€™s better to try with marker combinations, which were amenable for PCR and capable of distinguishing the varieties. Larger differences for each crop were found between cultivers from different seed companies than within the same company. These DNA markers can provide an easier and faster reliable genetic identification of rice cultivars.

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