Development of subgenome-specific PCR markers in the short arm of wheat and rye chromosome 1 and their utilization in wheat-rye translocation breeding

Euphytica ◽  
2021 ◽  
Vol 217 (7) ◽  
Author(s):  
Woo Joo Jung ◽  
Yong Weon Seo
Keyword(s):  
Chromosome 1 ◽  
Pcr Markers ◽  
Specific Pcr

scholarly journals Conversion of AFLP markers to sequence-specific PCR markers in barley and wheat

1999 ◽  
Vol 98 (6-7) ◽  
pp. 1072-1078 ◽  
Author(s):  
X. Shan ◽  
T. K. Blake ◽  
L. E. Talbert
Keyword(s):  
Aflp Markers ◽  
Pcr Markers ◽  
Specific Pcr

Toxigenic Fusarium species infecting wheat heads in Poland

2014 ◽  
Vol 9 (2) ◽  
pp. 163-172 ◽  
Author(s):  
Halina Wiśniewska ◽  
Łukasz Stępień ◽  
Agnieszka Waśkiewicz ◽  
Monika Beszterda ◽  
Tomasz Góral ◽  
...  
Keyword(s):  
Species Identification ◽  
High Temperatures ◽  
Fusarium Species ◽  
Common Species ◽  
Pcr Markers ◽  
High Rainfall ◽  
Central Poland ◽  
Specific Pcr

AbstractToxigenic Fusarium species are common pathogens of wheat and other cereals worldwide. In total, 449 wheat heads from six localities in Poland, heavily infected with Fusarium during 2009 season, were examined for Fusarium species identification. F. culmorum was the most common species (72.1% on average) with F. graminearum and F. avenaceum the next most commonly observed, but much less frequent (13.4 and 12.5% respectively). F. cerealis was found in 1.8% of all samples, and F. tricinctum was found only in one sample (0.2%). Subsequent quantification of the three major mycotoxins (deoxynivalenol, zearalenone and moniliformin) in grain and chaff fractions with respect to associated prevailing pathogen species uncovered the following patterns. Moniliformin (MON) was found in low amounts in all samples with F. avenaceum present. In contrast, deoxynivalenol (DON) and zearalenone (ZEA) were the contaminants of F. culmorum- and F. graminearum-infected heads. The highest concentration of DON was recorded in grain sample collected in Radzików (77 µg g−1). High temperatures in Central Poland during July and August accompanied with high rainfall in July were responsible for this high DON accumulation. Trichothecene, zearalenone, enniatin and beauvericin chemotypes were identified among 21 purified isolates using gene-specific PCR markers.

scholarly journals The variability of Venturia inaequalis CKE. races in the Czech republic and the accumulation of resistance genes in apple germplasm

2011 ◽  
Vol 50 (No. 9) ◽  
pp. 416-423 ◽  
Author(s):  
M. Melounová ◽  
P. Vejl ◽  
P. Sedlák ◽  
A. Reznerová ◽  
M. Tesařová ◽  
...  
Keyword(s):  
Czech Republic ◽  
Genetic Similarity ◽  
Venturia Inaequalis ◽  
Pcr Markers ◽  
The Czech Republic ◽  
Resistant Genotype ◽  
Vf Gene ◽  
Apple Varieties ◽  
Specific Pcr ◽  
Genetic Mechanisms

The growing of resistant apple varieties against the scab, impacts the Venturia inaequalis CKE. races development that can overcome the resistance. For this reason the main breeders object is to cumulate the different genetic mechanisms of resistance against this disease. Presented in this paper is the first genetic study of monosporic isolates in the Czech Republic. By means of RAPD and UPGMA methods which characterised the variability of 10 monosporic isolates from different localities and apple varieties. The monosporic isolate derived from the resistant genotype (Vf gene) proved a 79% genetic similarity with the isolate derived from sensitive variety Top Red. The genetic similarity of other isolates did not prove the dependence either on a locality or a host variety. The Vf and Vm genes accumulation in apple germplasm by means of specific PCR markers was studied. It was confirmed that Vf gene donors are always heterozygous. Concurrently it was statistically confirmed that the donor of Vm gene (OR-45-T-132) is heterozygous, too. The accumulation of Vf and Vm major genes against the scab was validated in 25% of seedlings of the cross.  

scholarly journals Rare Pyrenophora teres Hybridization Events Revealed by Development of Sequence-Specific PCR Markers

2017 ◽  
Vol 107 (7) ◽  
pp. 878-884 ◽  
Author(s):  
Barsha Poudel ◽  
Simon R. Ellwood ◽  
Alison C. Testa ◽  
Mark McLean ◽  
Mark W. Sutherland ◽  
...  
Keyword(s):  
Host Plants ◽  
Fragment Length Polymorphism ◽  
Putative Hybrid ◽  
Pyrenophora Teres ◽  
Net Blotch ◽  
Pcr Markers ◽  
Chain Reaction ◽  
Specific Pcr ◽  
Polymerase Chain ◽  
Pathogenic Variability

Pyrenophora teres f. teres and P. teres f. maculata cause net form and spot form, respectively, of net blotch on barley (Hordeum vulgare). The two forms reproduce sexually, producing hybrids with genetic and pathogenic variability. Phenotypic identification of hybrids is challenging because lesions induced by hybrids on host plants resemble lesions induced by either P. teres f. teres or P. teres f. maculata. In this study, 12 sequence-specific polymerase chain reaction markers were developed based on expressed regions spread across the genome. The primers were validated using 210 P. teres isolates, 2 putative field hybrids (WAC10721 and SNB172), 50 laboratory-produced hybrids, and 7 isolates collected from barley grass (H. leporinum). The sequence-specific markers confirmed isolate WAC10721 as a hybrid. Only four P. teres f. teres markers amplified on DNA of barley grass isolates. Amplified fragment length polymorphism markers suggested that P. teres barley grass isolates are genetically different from P. teres barley isolates and that the second putative hybrid (SNB172) is a barley grass isolate. We developed a suite of markers which clearly distinguish the two forms of P. teres and enable unambiguous identification of hybrids.

scholarly journals Development of diagnostic PCR and LAMP markers for MALE STERILITY 1 (MS1) in Cryptomeria japonica D. Don

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Yoichi Hasegawa ◽  
Saneyoshi Ueno ◽  
Fu-Jin Wei ◽  
Asako Matsumoto ◽  
Tokuko Ujino-Ihara ◽  
...  
Keyword(s):  
Male Sterility ◽  
Cryptomeria Japonica ◽  
Lamp Method ◽  
Pcr Markers ◽  
Causative Gene ◽  
Laboratory Equipment ◽  
Diagnostic Pcr ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

Abstract Objective Due to the allergic nature of the pollen of Cryptomeria japonica, the most important Japanese forestry conifer, a pollen-free cultivar is preferred. Mutant trees detected in nature have been used to produce a pollen-free cultivar. In order to reduce the time and cost needed for production and breeding, we aimed to develop simple diagnostic molecular markers for mutant alleles of the causative gene MALE STERILITY 1 (MS1) in C. japonica to rapidly identify pollen-free mutants. Results We developed PCR and LAMP markers to detect mutant alleles and to present experimental options depending on available laboratory equipment. LAMP markers were developed for field stations, where PCR machines are unavailable. The LAMP method only needs heat-blocks or a water bath to perform the isothermal amplification and assay results can be read by the naked eye. Because the causative mutations were deletions, we developed two kinds of PCR markers, amplified length polymorphism (ALP) and allele specific PCR (ASP) markers. These assays can be visualized using capillary or agarose gel electrophoresis.

The specific isolation of complete 5S rDNA units from chromosome 1A of hexaploid, tetraploid, and diploid wheat species using PCR with head-to-head oriented primers

Genome ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 529-538
Author(s):  
S Van Campenhout ◽  
J Vander Stappen ◽  
G Volckaert
Keyword(s):  
Ribosomal Rna ◽  
Diploid Species ◽  
5S Rdna ◽  
5S Rrna ◽  
Diploid Wheat ◽  
Wheat Species ◽  
Pcr Markers ◽  
Specific Pcr ◽  
A Genome ◽  
Sequence Types

The presence of 5S rDNA units on chromosome 1A of Triticum aestivum was shown by the development of a specific PCR test, using head-to-head oriented primers. This primer set allowed the amplification of complete 5S DNA units and was used to isolate 5S-Rrna-A1 sequences from polyploid and diploid wheat species. Multiple-alignment and parsimony analyses of the 132 sequences divided the sequences into four types. The isolates from T. aestivum and the tetraploid species (T. dicoccoides, T. dicoccum, T. durum, T. araraticum, and T. timopheevi) were all of one type, which was shown to be closely related to the type mainly characteristic for T. urartu. The other two types were isolated exclusively from the diploid species T. monococcum, T. aegilopoides, T. thaoudar, and T. sinskajae and the hexaploid species T. zhukovski. Triticum monococcum was the only species for which representatives of each of the four sequence types were found to be present. Further, we discuss the possible multicluster arrangement of the 5S-Rrna-A1 array.Key words: Triticum, A genome, PCR markers, 5S ribosomal RNA locus.

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