Population genetic structure of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) from Yunnan province (China) and nearby sites across the border

To infer the introduction sources of the oriental fruit fly, Bactrocera dorsalis, we used a mitochondrial marker to reconstruct the haplotype network and 15 microsatellite loci to reveal genetic structure and relationships between the geographically or temporally different collections from Asia. We performed Approximate Bayesian computations to infer a global origin and a source of the quarantine collections found in Korea. As a result, the 40 populations were divided into three groups, of which genetic similarity is not related to the geographic vicinity. Korean samples had a similar genetic structure to Taiwan and Thailand ones. Our results suggest that the place of origin of the B. dorsalis specimens found in Korea’s border quarantine is likely to be Taiwan or Thailand. As the global origin of B. dorsalis, we estimated that Taiwan and Thailand were most likely the global origins of Southeast Asian populations by testing hypothetical scenarios by the approximate Bayesian computation analyses. Our results will allow easier identification of the source region of the forthcoming invasion of quarantined B. dorsalis specimens.

Download Full-text

Issr marker based population genetic study of mediterranean fruit fly Ceratitis capitata (Diptera: Tephritidae)

Genetika ◽

10.2298/gensr2001311k ◽

2020 ◽

Vol 52 (1) ◽

pp. 311-322

Author(s):

Özge Kurd ◽

Ersin Doğaç ◽

Vatan Taşkin ◽

Belgin Göçmen-Taşkin

Keyword(s):

Genetic Diversity ◽

Genetic Structure ◽

Population Genetic Structure ◽

Mediterranean Basin ◽

Population Genetic ◽

Ceratitis Capitata ◽

Eastern Mediterranean ◽

Management Strategies ◽

Fruit Fly ◽

Eastern Mediterranean Basin

The Mediterranean fruit fly, Ceratitis capitata, is a serious pest of agricultural resources. Despite its economic importance, the population genetic structure of this species is still poorly investigated at micro-geographical level, especially from eastern Mediterranean basin. Knowledge about the genetic structure of C. capitata populations is a necessary requisite for understanding population history of the species and designing successful regional eradication programs. In the current study, the inter-simple sequences repeat (ISSR) markers were employed to assess the genetic diversity and population structure of seven natural populations of C. capitata that were collected from different regions of Turkey. Low to moderate levels of genetic diversity were observed. The estimated values for gene flow (Nm) and coefficient of genetic differentiation among populations (GST) were 3.07 and 0.14, respectively. The results of Principle Component Analysis (PCoA) and Unweighted Pair Group Arithmetic Mean Analysis (UPGMA) tend to be uniform in whole, the Antalya populations was clearly separated from the rest. Local environmental conditions, such as differences in pest control management strategies, agricultural practices, microclimates and human mediated transportations might be important factors in shaping the genetic structure of this species in Antalya. This paper provides useful data for understanding population genetic structure of C. capitata populations in eastern Mediterranean basin and development of effective regional pest management strategies.

Download Full-text

The Genetic Diversity of Bactrocera dorsalis (Diptera: Tephritidae) in China and Neighboring Countries: A Review From Published Studies

Journal of Economic Entomology ◽

10.1093/jee/toz073 ◽

2019 ◽

Vol 112 (4) ◽

pp. 2001-2006 ◽

Cited By ~ 1

Author(s):

Ivonne J Garzón-Orduña ◽

Scott M Geib ◽

Norman B Barr

Keyword(s):

Yunnan Province ◽

Fruit Fly ◽

Bactrocera Dorsalis ◽

Sampling Effort ◽

Oriental Fruit Fly ◽

Research Groups ◽

Mtdna Variation ◽

Mtdna Diversity ◽

Number Of Individuals ◽

Silent Substitutions

Abstract For more than a decade, various research groups have tracked the population genetics of the oriental fruit fly, Bactrocera dorsalis (Hendel) in China and neighboring countries using mitochondrial cytochrome c oxidase subunit I (COI) DNA. Although most research has reported high levels of mtDNA variation, to date no efforts have been made to integrate and compare the results from these studies simultaneously. Here, we show that: 1) despite the fact that a large portion of the sampling effort has focused on the Yunnan province beginning in 2005, each subsequent study recovers only a small number of previously sampled haplotypes; 2) new haplotypes of B. dorsalis remain to be found, a projection of new haplotypes versus the number of individuals sampled suggest that sampling the species mtDNA diversity is far from reaching an asymptote; 3) it is unlikely that the observed genetic variation is the result of NUMTs (nuclear mitochondrial DNA), as most differences between haplotypes are silent substitutions; and 4) although all studies employed the 3′ end of COI, the length of COI fragment sequenced differs among studies, making comparisons challenging. Therefore, we offer these results with the caveat that mtDNA diversity might be underestimated in China.

Download Full-text