Twenty five tomato (Solanum lycopersicum L.) genotypes were subjected to genetic diversity analysis using twenty SSR markers. Out of 20 markers used, 14 SSRs were polymorphic and a total numbers of 22 SSR alleles were generated by 14 SSR markers, out of which 19 were polymorphic and 3 were monomorphic, with an average of 1.57 alleles per locus. The range of amplified products was 100-400bp approximately. Jaccard’s similarity coefficient varied from 0.65 between germplasm EC519821 and CO-3 to a maximum of 1.0 between genotypes EC519769 and DARL-66, with an average value of 0.83. Cluster analysis based on Jaccard’s similarity coefficient using the unweighted pair-group method with arithmetic mean (UPGMA) revealed 2 distinct clusters, A and B, comprising 1 and 24 genotypes respectively and at 75 and 78 per cent similarity, respectively. The genotypes which showed similar morphological and genetic trends were grouped more or less together in both these cases were a few. Cluster A comprised most diverse germplasm (EC519821)belongs to pimpinellifolium wild species with similarity coefficient 0.65% and differentiated with other cultivated species.Cherry Tomato and Cherry-2 were trends in similar cluster similar with approximately 96% similarity.SSR markers were able in in differentiating the genotypes based on morphologically and genotypically.However, the grouping of 25 genotypes were independently of geographic distribution.The genetic distance information found in this study might be helpful to breeder for planning among these genotypes.
Comparison of gSSR and EST-SSR markers for analyzing genetic variability among tomato cultivars (Solanum lycopersicum L.)
