Understanding genetic diversity in blackgram [Vigna mungo (L.) Hepper] collections of Indian National Genebank

The DNA fingerprinting methodologies, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were used to estimate genetic diversity and relationships among 20 black gram (Vigna mungo L. Hepper) varieties. Thirty selected RAPD primers amplified 255 bands, 168 of which were polymorphic (66.5%). On average, these primers produced 8.5 bands, 5.6 of which were polymorphic. Polymorphic band number varied from 2 (A-05) to 10 (OPA-02), with sizes ranging from 100 to 2550 bp. Twenty-four selected ISSR primers produced 238 amplified products, 184 of which were polymorphic (77.8%). On average, these primers generated 9.8 bands, with 7.7 polymorphic bands ranging in number from 4 (ISSR-13) to 11 (ISSR-03), and size from 100-2650 bp. Genetic relationships were estimated using similarity coefficient (Jaccard’s) values between different accession pairs; these varied from 30.7 to 85.0 for RAPD, and from 37.2 to 88.4 with ISSR. UPGMA analysis indicated that the varieties ranged in similarity from 0.50 to 1.00 (mean of 0.75) for RAPD, and from 0.47 to 1.00 (mean of 0.76) with ISSR. Cluster analysis of RAPD and ISSR results identified three clusters with significant bootstrap values, which revealed greater homology between the varieties. Principal coordinates analysis also supported this conclusion. Among the black gram varieties, WBU-108 and RBU-38 were highly divergent, whereas LBG-648 and LBG-623 were genetically similar. The markers generated by RAPD and ISSR assays can provide practical information for the management of genetic resources and these results will also provide useful information for the molecular classification and breeding of new black gram varieties.Key words: Black gram, cluster analysis, genetic diversity, ISSR, molecular markers, RAPD

Download Full-text

Genetic diversity analysis in blackgram [Vigna mungo (L.) Hepper]

Electronic Journal of Plant Breeding ◽

10.37992/2021.1201.006 ◽

2021 ◽

Vol 12 (01) ◽

Keyword(s):

Genetic Diversity ◽

Vigna Mungo ◽

Diversity Analysis ◽

Genetic Diversity Analysis

Download Full-text

Study of Genetic Diversity for Yield and Yield Contributing Traits in Black Gram (Vigna mungo L.)

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2020.907.270 ◽

2020 ◽

Vol 9 (7) ◽

pp. 2316-2319

Author(s):

Deeksha Chandrakar ◽

Abhinav Sao ◽

Deepak Gauraha

Keyword(s):

Genetic Diversity ◽

Vigna Mungo ◽

Black Gram

Download Full-text

Frequency of heterotic hybrids in relation to parental genetic divergence and general combining ability in blackgram [Vigna mungo (L.) Hepper]

Legume Research - An International Journal ◽

10.18805/lr-3916 ◽

2019 ◽

Author(s):

K.M. Boraiah ◽

M. Byregowda ◽

C.M. Keerthi ◽

H.P. Vijayakumar ◽

S. Ramesh ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Divergence ◽

Combining Ability ◽

General Combining Ability ◽

Vigna Mungo ◽

Per Se

An investigation was carried out to test the predictability of frequency of heterotic hybrids based on parental gca effects and genetic diversity in blackgram. The 40 F1s effected using 10 lines and 4 testers were evaluated along with their parents for 10 productivity per set raits. The overall gca status (high and low) of each parent and overall sca and heterotic status (high and low) of each hybrid across 10 traits were determined. Based on overall gca status and genetic divergence of parents, the hybrids were grouped into different classes. The hybrids involving parents contrasting for overall gca status and/or those involving parents with intermediate genetic divergence were more frequently heterotic than those involving comparable gca status and with extreme genetic divergence. It is hence, desirable to involve parents with intermediate genetic divergence and contrasting gca effects to recover higher frequencies of heterotic hybrids for productivity per se traits in blackgram.

Download Full-text

Genetic diversity analysis in blackgram (Vigna mungo (L.) Hepper) using AFLP and transferable microsatellite markers from azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi)

Genome ◽

10.1139/g08-107 ◽

2009 ◽

Vol 52 (2) ◽

pp. 120-129 ◽

Cited By ~ 21

Author(s):

S. K. Gupta ◽

T. Gopalakrishna

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Information Content ◽

Principal Components ◽

Polymorphic Information Content ◽

Mantel Test ◽

Vigna Mungo ◽

Azuki Bean ◽

Vigna Angularis ◽

Aflp Data

Genetic diversity in 20 elite blackgram ( Vigna mungo (L.) Hepper) genotypes was studied using microsatellite and AFLP markers. Thirty-six microsatellite markers from azuki bean ( Vigna angularis (Willd.) Ohwi & Ohashi) were successfully amplified across the 20 blackgram genotypes and 33 microsatellite markers showed polymorphism. A total of 137 microsatellite alleles were generated with an average of 4.1 alleles per locus. The number of alleles ranged from two to nine and the polymorphic information content value for the microsatellite markers varied from 0.10 to 0.87 with an average of 0.49. Microsatellite markers were highly informative and a combination of only three microsatellite markers (CEDG264, CEDG173, and CEDG044) was sufficient to discriminate all 20 blackgram genotypes. In the case of AFLP, 11 primer pairs generated 324 polymorphic marker fragments. The polymorphic information content values for AFLP primer combinations ranged from 0.21 to 0.34 with an average of 0.29. Similarity measures and clustering analyses were made using microsatellite and AFLP data separately. The resulting dendrograms distributed the 20 blackgram genotypes into five main clusters. The dendrograms were comparable with each other with the Mantel test between the cophenetic matrices of microsatellite data and AFLP data showing moderate correlation (r = 0.64). The results of the principal components analysis were well congruent with the dendrograms. In the dendrograms as well as in the principal components analyses, genotype Trombay wild (Vigna mungo var. silvestris) was placed separately from rest of the genotypes. This study demonstrated that the azuki bean microsatellite markers are highly polymorphic and informative and can be successfully used for genome analysis in blackgram. Results indicate that sufficient variability is present in the blackgram genotypes and would be helpful in the selection of suitable parents for breeding purposes and gene mapping studies.

Download Full-text

Assessment of Genetic Diversity in Blackgram [Vigna mungo (L.) Hepper] Genotypes Evaluated under Different Seasons

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2020.910.106 ◽

2020 ◽

Vol 9 (10) ◽

pp. 884-890

Author(s):

Sidram appa ◽

P. H. Kuchanur ◽

M. Shobharani ◽

B. Arunkumar ◽

S. A. Kulkarni ◽

...

Keyword(s):

Genetic Diversity ◽

Vigna Mungo ◽

Different Seasons

Download Full-text

Genetic Diversity Analysis in Black Gram (Vigna mungo L. Hepper)

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2021.1001.157 ◽

2021 ◽

Vol 10 (01) ◽

pp. 1321-1328

Author(s):

Dondiba Kundagar ◽

N. Brajendra Singh ◽

M. Samuel Jeberson ◽

Bireswar Sinha ◽

N. Gopimohan Singh

Keyword(s):

Genetic Diversity ◽

Vigna Mungo ◽

Diversity Analysis ◽

Black Gram ◽

Genetic Diversity Analysis

Download Full-text

Nature of genetic diversity for seed yield and its component traits in urdbean [Vigna mungo (L.) Hepper]

Indian Journal of Agricultural Research ◽

10.18805/ijare.v50i1.8592 ◽

2016 ◽

Vol 50 (1) ◽

Author(s):

P. Y. Kamannavar ◽

S. B. Revanappa ◽

A. G. Vijaykumar ◽

K. Basamma ◽

Ganajaxi

Keyword(s):

Genetic Diversity ◽

Plant Height ◽

Seed Yield ◽

Morphological Traits ◽

Vigna Mungo ◽

Breeding Lines ◽

Cluster Distance ◽

Component Traits

Genetic diversity was assessed in thirty urdbean cultivars including advance breeding lines by Mahalanobis D<sup>2</sup> statistics using seed yield and its component morphological traits. Analysis of genetic diversity revealed considerable amount of diversity among the genotypes and led to their grouping into eight clusters. The cluster-I comprising 14 genotypes was the largest followed by cluster-II and III with 5 and 4 genotypes respectively. The remaining clusters were monogenotypic. Pods per plant (32.5%), seed yield per plant (30%) and plant height (21.5%) were mainly responsible for genetic diversity. Based on inter-cluster distance and mean performance of clusters for different traits, the crosses among genotypes like, DBG-5, DU-1, Manikya, KU-7638, and DBG-3 are expected to produce desirable segregants for yield and other yield related traits.

Download Full-text

Assessment of genetic diversity in black gram [Vigna mungo (L.) Hepper] genotypes based on ISSR

Legume Research - An International Journal ◽

10.18805/lr-3401 ◽

2018 ◽

Author(s):

Divya Vyas ◽

Arunabh Joshi ◽

Ganesh Rajamani ◽

Devendra Jain

Keyword(s):

Genetic Diversity ◽

Phylogenetic Relationship ◽

Genetic Divergence ◽

Vigna Mungo ◽

Issr Marker ◽

Black Gram ◽

Versatile Tool ◽

Issr Primers

A study was carried out among 22 black gram genotypes to study the genetic diversity using 20 ISSR primers. Screening of the primers revealed that only 15 out of the 20 primers produced amplification. A total of 84 amplified bands were obtained, out of which 72 were polymorphic 85.71 percent polymorphism. The total number of amplified bands varied between 1 (UBC-813 and UBC-878) and 9 (UBC-826) with an average of 5 bands per primer. The overall size of PCR amplified products ranged between 250 bp to 2000 bp. PIC values ranged from 0.00 to 0.51 with an average of 0.285 across all genotypes. Five unique bands were detected in four genotypes, out of which the genotype U-9 gave maximum number of distinct bands. The size of these unique bands ranged from 450 bp to 2000 bp. Based on the UPGMA derived dendrogram and PCA, the 22 genotypes could be divided into four main clusters. While Cluster I included 16 genotypes, the Clusters II, III and IV included two genotypes each. Genotypes IC-16511 and UTTARA, UH-177 and IPU2K-21, STY-2834 and UH-177 were found to be genetically distant from each other with a minimum similarity value of 0.42. The results are encouraging with the suggestion that the ISSR marker could prove to be a versatile tool in further screening of the Vigna germplasm pool for study of genetic divergence and the establishment of phylogenetic relationship amongst accessions.

Download Full-text