Manipulating the expression of a cell wall invertase gene increases grain yield in maize

2017 ◽  
Vol 84 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Yu-Jing Bi ◽  
Zhen-Cang Sun ◽  
Jessie Zhang ◽  
En-Qi Liu ◽  
Han-Mei Shen ◽  
...  
Keyword(s):  
Cell Wall ◽  
Grain Yield ◽  
Cell Wall Invertase ◽  
A Cell ◽  
Invertase Gene

scholarly journals Induction of a Pea Cell-Wall Invertase Gene by Wounding and Its Localized Expression in Phloem

1996 ◽  
Vol 112 (3) ◽  
pp. 1111-1117 ◽  
Author(s):  
L. Zhang ◽  
N. S. Cohn ◽  
J. P. Mitchell
Keyword(s):  
Cell Wall ◽  
Cell Wall Invertase ◽  
Invertase Gene

Roles played by invertase and gene expression in the development of the horn-shaped gall on leaves of Rhus chinensis

2017 ◽  
Vol 44 (12) ◽  
pp. 1160 ◽  
Author(s):  
Zhen-Yuan Ruan ◽  
Xiao-Ming Chen ◽  
Pu Yang ◽  
Bing-Yi Wang
Keyword(s):  
Cell Wall ◽  
Invertase Activity ◽  
Activity Level ◽  
Rapid Expansion ◽  
Sink Strength ◽  
Cell Wall Invertase ◽  
Vacuolar Invertase ◽  
Growing Period ◽  
Rhus Chinensis ◽  
Invertase Gene

The present study deals with the growth and development of the horn-shaped gall, which is induced by Schlechtendalia chinensis Bell. on leaves of Rhus chinensis Mill. The relationship between gall formers and their host plants was investigated by means of the activities of various invertases, the expressions of the cell wall invertase gene (INV2), and vacuolar invertase gene (INV3) during gall development. Our results show that the increase in the sink strength of the galls required cell wall invertase and vacuolar invertase, and that vacuolar invertase had a particular impact during the early development. In addition, vacuolar invertase activity was always significantly higher in galls than in leaves. However, ionically bound cell wall invertase showed a slightly significant increased activity level when compared with the leaves after galls had entered the fast growing period. This result indicates that vacuolar invertase is related to the rapid expansion of the galls, but ionically bound cell wall invertase is involved in the rapid growth of tissues. The enhanced activity of cell wall invertase and the expression of INV2 may be a plant response to a gall-induced stress. Cytoplasmic invertase that acts as a maintenance enzyme, or takes part in the production of secondary metabolites, was elevated when intracellular acid invertase activity decreased.

cDNA cloning, heterologous expression and characterization of a cell wall invertase from copper tolerant population of Elsholtzia haichowensis

Biologia ◽  
2015 ◽  
Vol 70 (8) ◽  
Author(s):  
Chen Liu ◽  
Zhongrui Xu ◽  
Shenwen Cai ◽  
Luan Zhang ◽  
Zhiting Xiong
Keyword(s):  
Cell Wall ◽  
Evolutionary Relationship ◽  
Methylotrophic Yeast ◽  
Cell Wall Invertase ◽  
Reading Frame ◽  
Methylotrophic Yeast Pichia Pastoris ◽  
Acid Protein ◽  
Elsholtzia Haichowensis ◽  
A Cell ◽  
Theoretical Molecular Mass

AbstractThe main objective of the present study was to clone, heterologously express and characterize a novel cell wall invertase (FCWI) from a Cu tolerant population of Elsholtzia haichowensis. The full-length FCWI cDNA contained an open reading frame (ORF) of 1671 bp which encoded a 556-amino-acid protein. The theoretical molecular mass and pI of the deduced protein were 62.5 kDa and 9.29, respectively. Phylogenetic analysis showed that FCWI had a closer evolutionary relationship to cell wall invertase of dicot. FCWI was expressed in methylotrophic yeast Pichia pastoris and purified to near homogeneity. Recombinant FCWI enzyme had pH optima of 4.0 and temperature optima of 50◦C. Activity analyses in the presence of various metal cations indicated that FCWI was completely inhibited by Hg

Isolation, Characterization and Promoter Analysis of Cell Wall Invertase Gene SoCIN1 from Sugarcane (Saccharum spp.)

Sugar Tech ◽  
2014 ◽  
Vol 17 (1) ◽  
pp. 65-76 ◽  
Author(s):  
Jun-Qi Niu ◽  
Ai-Qin Wang ◽  
Jing-Li Huang ◽  
Li-Tao Yang ◽  
Yang-Rui Li
Keyword(s):  
Cell Wall ◽  
Promoter Analysis ◽  
Cell Wall Invertase ◽  
Saccharum Spp ◽  
Invertase Gene

Cloning and Analysis of MeCWINV6 Promoter from Biofuel Plant Cassava (Manihot esculenta Crantz)

2014 ◽  
Vol 986-987 ◽  
pp. 25-29
Author(s):  
Jiao Liu ◽  
Wen Rui Xia ◽  
Yan Ping Hu ◽  
Yuan Yao ◽  
Shao Ping Fu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pcr Amplification ◽  
Promoter Sequence ◽  
Starch Accumulation ◽  
Cell Wall Invertase ◽  
Potential Promoter ◽  
Invertase Gene ◽  
Responsive Element ◽  
Source Sink ◽  
Insight Into

In order to gain insight into the specific function of the cassava cell wall invertase 6 (MeCWINV6), the promoter sequence of MeCWINV6 gene was cloned using the PCR amplification approach. 118 bp CDS sequence and 1042 bp potential promoter sequence of MeCWINV6 gene were obtained. PlantCARE analyzed the putative cis-elements in silico revealed that these elements can be grouped into five classes: basic transcription elements (CAAT box and TATA box), light responsive elements (ACE, AE-box, ATCT-motif, AT1-motif, Box 4, GAG-motif, GT1-motif and Sp1), phytohormone responsive motifs (GARE-motif, TATC-box, TGACG-motif and TCA-element), defense and stress responsive element (TC-rich repeats and HSE), wounding and pathogen responsive elements (W-box and WUN-motif). This data demonstrate that it might be associated to regulate the cell wall invertase gene function in source-sink relations of cassava starch accumulation and response to internal and environmental stimuli.

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