Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-γ

Inflammation ◽  
2022 ◽  
Author(s):  
Catherine S. Coleman ◽  
Bruce A. Stanley ◽  
Charles H. Lang
Keyword(s):  
Interferon Γ ◽  
C2c12 Myotubes

scholarly journals NO synthase II in mouse skeletal muscle is associated with caveolin 3

1999 ◽  
Vol 340 (3) ◽  
pp. 723-728 ◽  
Author(s):  
Ingolf GATH ◽  
Jutta EBERT ◽  
Ute GÖDTEL-ARMBRUST ◽  
Ralf ROSS ◽  
Angelika B. RESKE-KUNZ ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Interferon Γ ◽  
No Synthase ◽  
C2c12 Myotubes ◽  
Muscle Fibres ◽  
Wild Type ◽  
Regulation Of Contraction ◽  
Slow Twitch ◽  
Co Precipitation ◽  
Deficient Mice

The inducible-type NO synthase (NOS II; iNOS) is constitutively expressed in slow-twitch skeletal muscle fibres of guinea-pigs [Gath, Closs, Gödtel-Armbrust, Schmitt, Nakane, Wessler and Förstermann (1996) FASEB J. 10, 1614-1620]. Here we studied the expression of NOS II in skeletal muscle of wild-type and NOS II-deficient mice and investigated the molecular basis for the membrane association of this NOS in muscle. A basal expression of NOS II mRNA and protein was detected in skeletal muscle from untreated wild-type mice; expression increased when mice were treated with bacterial lipopolysaccharide (LPS). No NOS II was found in any tissue of untreated or LPS-treated NOS II-deficient mice. Immunoprecipitation experiments were performed with homogenates of gastrocnemius muscle from untreated or LPS-treated wild-type mice. A NOS II-specific antibody precipitated caveolin 3 in all homogenates investigated, the effect being most pronounced in skeletal muscle from LPS-treated animals. Conversely, an antibody against caveolin 3 co-precipitated NOS II in muscle homogenates. Similarly, a weak co-precipitation of NOS II and caveolin 3 was seen in homogenates of untreated murine C2C12 myotubes; co-precipitation was markedly enhanced in cells stimulated with LPS/interferon γ. The association of NOS II with caveolin 3 might have implications for the regulation of contraction of, and/or glucose uptake by, slow-twitch muscle fibres.

Colocalization but differential regulation of neuronal NO synthase and nicotinic acetylcholine receptor in C2C12 myotubes

2003 ◽  
Vol 284 (4) ◽  
pp. C1065-C1072 ◽  
Author(s):  
Jutta G. Ebert ◽  
Marek Zelenka ◽  
Ingolf Gath ◽  
Ute Gödtel-Armbrust ◽  
Ulrich Förstermann
Keyword(s):  
Skeletal Muscle ◽  
Acetylcholine Receptor ◽  
Nicotinic Acetylcholine Receptor ◽  
Specific Antibody ◽  
Interferon Γ ◽  
C2c12 Myotubes ◽  
Mammalian Skeletal Muscle ◽  
Nicotinic Acetylcholine ◽  
Protein Levels ◽  
Murine Skeletal Muscle

In mammalian skeletal muscle, neuronal-type nitric oxide synthase (nNOS) is found to be enriched at neuromuscular endplates. Here we demonstrate the colocalization of the nicotinic acetylcholine receptor (nAChR, stained with α-bungarotoxin) and nNOS (stained with a specific antibody) in murine C2C12 myotubes. However, coimmunoprecipitation experiments demonstrated no evidence for a direct protein-protein association between the nAChR and nNOS in C2C12myotubes. An antibody to the α1-subunit of the nAChR did not coprecipitate nNOS, and an nNOS-specific antibody did not precipitate the α1-subunit of the nAChR. Treatment of mice with bacterial LPS downregulated the expression of nNOS in skeletal muscle, and treatment of C2C12 cells with bacterial LPS and interferon-γ markedly decreased nNOS mRNA and protein expression. In contrast, mRNA and protein of the nAChR (α-, γ-, and ε-subunits) remained unchanged at the mRNA and protein levels. These data demonstrate that nNOS and the nAChR are colocalized in murine skeletal muscle and C2C12 cells but differ in their expressional regulation.

Interferon γ mediated induction of apoptosis in primary human neuroendocrine tumor cells

2001 ◽  
Vol 120 (5) ◽  
pp. A509-A509
Author(s):  
A DROST ◽  
J KEHRBERGER ◽  
U PLOECKINGER ◽  
B WIEDENMANN ◽  
S ROSEWICZ ◽  
...  
Keyword(s):  
Neuroendocrine Tumor ◽  
Tumor Cells ◽  
Interferon Γ ◽  
Induction Of Apoptosis

Regulation of somatostatin and gastrin by interferon-γ in the mouse gastric mucosa

2001 ◽  
Vol 120 (5) ◽  
pp. A727-A727
Author(s):  
Y ZAVROS ◽  
J MERCHANT
Keyword(s):  
Gastric Mucosa ◽  
Interferon Γ

Author response for "Cell surface IL‐1α trafficking is specifically inhibited by interferon‐γ, and associates with the membrane via IL‐1R2 and GPI anchors"

2020 ◽  
Author(s):  
Julie NE. Chan ◽  
Melanie Humphry ◽  
Lauren Kitt ◽  
Dominika Krzyzanska ◽  
Kara J. Filbey ◽  
...  
Keyword(s):  
Cell Surface ◽  
Interferon Γ ◽  
Author Response
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