Assessment of Infections with Microsporidia and Cryptosporidium spp. in Fecal Samples from Wild Primate Populations from Cameroon and Democratic Republic of Congo

2015 ◽  
Vol 36 (2) ◽  
pp. 227-243 ◽  
Author(s):  
Christelle Butel ◽  
Steve Ahuka Mundeke ◽  
Pascal Drakulovski ◽  
Donika Krasteva ◽  
Eitel Mpoudi Ngole ◽  
...  
2015 ◽  
Vol 60 (4) ◽  
Author(s):  
Roger D. Wumba ◽  
Josué Zanga ◽  
Kennedy M. Mbanzulu ◽  
Madone N. Mandina ◽  
Aimé K. Kahindo ◽  
...  

AbstractCryptosporidium is an important protozoan parasite affecting HIV/AIDS patients. To determine the prevalence and the species of Cryptosporidium spp by developing a rapid and efficient real-time PCR-RFLP test. A cross-sectional study was conducted among HIV-infected adults from Kinshasa, the Democratic Republic of Congo. Stool specimens were examined by microscopic evaluation and real-time PCR-RFLP. Out of 242 HIV-infected adults, 10 (4.1%) cases of Cryptosporidium were identified by microscopic examination. Using PCR-RFLP, the prevalence of Cryptosporidium spp was 5.4% (n = 13). All the 13 cases of Cryptosporidium spp had the stage of AIDS of HIV infection. Extracted DNA was amplified by nested PCR targeting a 1030-bp fragment of the 18s RNA gene. RFLP analysis identified one C. parvum, four C. hominis and one non determined Cryptosporidium. The capacity to detect C. parvum, C. hominis and non-determined Cryptosporidium was present among our HIV-infected patients.


2020 ◽  
Author(s):  
Richard Chamboko ◽  
Robert Cull ◽  
Xavier Gine ◽  
Soren Heitmann ◽  
Fabian Reitzug ◽  
...  

2015 ◽  
Vol 8 (1) ◽  
pp. 1485-1497
Author(s):  
Mia Nsokimieno Misilu Eric

The current state of large cities in Democratic Republic of Congo highlights the necessity of reinventing cities. More than fifty years after the independence, these major cities, like Kinshasa the capital city, are in a state of are in a state of disrepair. They are damaged, dysfunctional, and more vulnerable. Today, these legacy cities do not meet the international requirements of livable cities. Democratic Republic of Congo faces the challenge of rebuilding its cities for sustainability. The movement for independence of African countries enabled the shift from colonial cities to legacy cities. It is important to understand the cultural and ideological foundations of colonial city. Commonly, colonial cities served as purpose-built settlements for the extraction and transport of mineral resources toward Europe. What's required is a creative reconstruction to achieve a desired successful urban change. Creative reconstruction tends to ensure urban transformation in relation with urbanization, by making continuous and healthy communities. Creative reconstruction seems appropriate way of building back cities in harmony with cultural values. The article provides a framework for urban regeneration. The study is based on principle of thinking globally and acting locally in building back better cities.


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