Development and Validation of a First-Derivative Spectrophotometric Method for the Estimation of an Antipsychotic Drug in Pharmaceutical Formulations and Forced Degradation Studies

2022 ◽  
Author(s):  
S. K. Dash ◽  
S. K. Acharjya ◽  
P. S. Das ◽  
N. K. Kumar ◽  
Ch. N. Patra
Keyword(s):  
Antipsychotic Drug ◽  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Forced Degradation ◽  
First Derivative ◽  
Forced Degradation Studies ◽  
Degradation Studies ◽  
Development And Validation

scholarly journals METHOD DEVELOPMENT AND VALIDATION OF FORCED DEGRADATION STUDIES OF CARVEDILOL BY USING UV SPECTROSCOPY

2016 ◽  
Vol 03 (02) ◽  
pp. 53-56
Author(s):  
Kamalakannan Dhanapal ◽  
Ananda T Subramaniam ◽  
Anandakumar Karunakaran ◽  
Jambulingam Munusamy ◽  
Devi Velmurugan
Keyword(s):  
Method Development ◽  
Uv Spectroscopy ◽  
Forced Degradation ◽  
Forced Degradation Studies ◽  
Method Development And Validation ◽  
Degradation Studies ◽  
Development And Validation

scholarly journals An Eco-friendly RP-HPLC and UV-Method Development and Validation for an Estimation of Tolvaptan in Bulk and Tablet Dosage form Followed by Forced Degradation Studies

2021 ◽  
pp. 271-286
Author(s):  
Patel Seema A. ◽  
Sayyed Nazifa S. ◽  
Lajporiya Mobina I. ◽  
Manjra Mehfuza U. ◽  
Aejaz Ahmed ◽  
...  
Keyword(s):  
Dosage Form ◽  
Method Development ◽  
Forced Degradation ◽  
Rp Hplc ◽  
Drug Concentrations ◽  
Forced Degradation Studies ◽  
Method Development And Validation ◽  
Degradation Studies ◽  
Development And Validation ◽  
Tablet Dosage

Aims: To develop and validate a new, simple, rapid, precise, and accurate An Eco-friendly RP-HPLC and UV-Method Development and Validation for an estimation of Tolvaptan in Bulk and Tablet dosage form followed by Forced Degradation Studies Place and Duration of the Study: The present work has been carried out at Ali-Allana College of Pharmacy, Akkalkuwa between November-2020 to April-2021. Methodology: The UV-Spectroscopic method was developed for the estimation of tolvaptan in bulk and tablet dosage form. The solvent selected for the tolvaptan UV analysis was 4% aq. SLS solution, the solution of 10µg/ml was scanned in UV region from 200-400 nm and the λmax value was determined. The RP-HPLC method was developed on Sunsil C18 150 mm x 4.6mm x 5μ column using acetonitrile: water [45:55] as mobile phase at flow rate 1.0 ml/min and UV detection at 266 nm. Results: The maximum absorbance was observed at 266 nm. The wavelength 266 nm was selected for further analysis of tolvaptan. The calibration curve was determined using drug concentrations ranging from 20-100 µgm/ml. The system suitability was performed by injecting a standard solution containing 200µg/ml of tolvaptan in six replicates. For two of them, the peak asymmetric were <1.5 and the theoretical plate number was >2000, and the %RSD of tolvaptan was less than 2. Conclusion: From the above results, it was concluded that the developed UV and RP-HPLC methods are precise and accurate and can be applied for the quantitative estimation of tolvaptan from bulk and tablet dosage forms. The method can be used for routine testing of tolvaptan by the pharmaceutical industry. Validation of the developed method was done as per International Conference on Harmonization (ICH) Q2R1 guidelines.

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