In vitro evaluation of folic acid modified carboxymethyl chitosan nanoparticles loaded with doxorubicin for targeted delivery

2010 ◽  
Vol 21 (5) ◽  
pp. 1587-1597 ◽  
Author(s):  
Sumanta K. Sahu ◽  
Sanjay K. Mallick ◽  
Susmita Santra ◽  
Tapas K. Maiti ◽  
Sudip K. Ghosh ◽  
...  
Keyword(s):  
Folic Acid ◽  
Targeted Delivery ◽  
Chitosan Nanoparticles ◽  
Carboxymethyl Chitosan ◽  
In Vitro Evaluation

scholarly journals Carboxymethyl chitosan nanoparticles coupled with CD59-specific ligand peptide for targeted delivery of C-phycocyanin to HeLa cells

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769226 ◽  
Author(s):  
Peng Yang ◽  
Bing Li ◽  
Qi-Feng Yin ◽  
Yu-Juan Wang
Keyword(s):  
Hela Cells ◽  
Targeted Delivery ◽  
Drug Loading ◽  
Chitosan Nanoparticles ◽  
Inhibitory Effect ◽  
Carboxymethyl Chitosan ◽  
Synthesis Conditions ◽  
Novel Approach ◽  
Specific Ligand

The combination of nanotechnology and medicine will be the next generation of vehicles for targeted drug delivery. Carboxymethyl chitosan loaded with the anticancer drug C-phycocyanin and the CD59-specific ligand peptide for cancer cell targeting were used to create C-phycocyanin/carboxymethyl chitosan–CD59-specific ligand peptide nanoparticles using the ionic-gelation method. Optimal synthesis conditions, selected by response surface methodology, comprised the ratio carboxymethyl chitosan:C-phycocyanin = 3:1, and carboxymethyl chitosan and CaCl2 concentrations of 2.0 and 1.0 mg/mL, respectively. The resulting nanoparticles were spherical, with diameters of approximately 200 nm; the entrapment efficient was about 65%; and the drug loading was about 20%. The release of C-phycocyanin from C-phycocyanin/carboxymethyl chitosan nanoparticles was pH sensitive and had a sustainable effect in vitro. Guided by the CD59-specific ligand peptide, the nanoparticles efficiently targeted the surface of HeLa cells and had an obvious inhibitory effect on HeLa cell proliferation as determined by methyl thiazolyl tetrazolium assays. The nanoparticles were hemocompatible and induced apoptosis by upregulation of cleaved caspase-3 and cleaved polyADP-ribose polymerase proteins, and downregulation of Bcl-2 proteins. Our study provides a novel approach to the research and development of marine drugs, and support for targeted therapy using anticancer drugs.

Preparation and in vitro evaluation of FGF-2 incorporated carboxymethyl chitosan nanoparticles

2017 ◽  
Vol 173 ◽  
pp. 114-120 ◽  
Author(s):  
Cong-Thuan Nguyen ◽  
Thanh-Thao Nguyen ◽  
Thu-Thuy Nguyen ◽  
Phuong Phong Thi Nguyen ◽  
Anh Dzung Nguyen ◽  
...  
Keyword(s):  
Chitosan Nanoparticles ◽  
Carboxymethyl Chitosan ◽  
In Vitro Evaluation
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