scholarly journals Stromal cell-derived factor (SDF)-1α and platelet-rich plasma enhance bone regeneration and angiogenesis simultaneously in situ in rabbit calvaria

2021 ◽  
Vol 32 (9) ◽  
Author(s):  
Zhengye Zhang ◽  
Yang Zheng ◽  
Jianing Zu ◽  
Jinpeng Zhuang ◽  
Gongping Xu ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Stromal Cell ◽  
Synergistic Effects ◽  
Platelet Rich Plasma ◽  
Group Versus ◽  
Autogenous Bone ◽  
Treatment Regimens ◽  
Stromal Cell Derived Factor ◽  
Bone Particles

AbstractThe current study aimed to evaluate the effects of chemokine stromal cell-derived factor (SDF)-1α and platelet-rich plasma (PRP) on bone formation and angiogenesis, and to assess whether SDF-1α and PRP could function synergistically. Four evenly distributed defects (8 mm in diameter) were generated in the calvarial bones of New Zealand white rabbits. All rabbits received four treatment regimens containing autogenous bone particles (AB), SDF-1α, or PRP. AB group presented significantly less bone formation compared with the other three groups 2 and 4 weeks after surgery. The amount of newly formed bone in the AB+PRP+SDF-1α group was similar to that in the AB + SDF-1α group at the 4-week time-point (p = 0.65), and was much greater than that in the AB and AB+PRP group (p < 0.001). Meanwhile, more new blood vessels were formed in the AB+PRP, AB+SDF-1α, and AB+PRP+SDF-1α group versus the AB group. AB+PRP+SDF-1α group showed statistically increased angiogenesis compared with the AB+PRP and AB+SDF-1α groups (both p < 0.05) after treatment for 2 and 4 weeks. These findings indicated that SDF-1α and PRP might exhibit synergistic effects to promote angiogenesis in early bone regeneration.

The potential of stromal cell-derived factor-1 delivery using a collagen membrane for bone regeneration

2017 ◽  
Vol 31 (7) ◽  
pp. 1049-1061 ◽  
Author(s):  
Tadahiro Takayama ◽  
Jisen Dai ◽  
Keita Tachi ◽  
Ryutaro Shohara ◽  
Hironori Kasai ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Stromal Cell ◽  
In Vitro Studies ◽  
New Bone Formation ◽  
Stromal Cell Derived Factor

Stromal cell-derived factor-1 (SDF-1) is a cytokine that is important in stem and progenitor cell recruitment in tissue repair after injury. Regenerative procedures using collagen membranes (CMs) are presently well established in periodontal and implant dentistry. The objective of this study is to test the subsequent effects of the released SDF-1 from a CM on bone regeneration compared to platelet-derived growth factor (PDGF) in vitro and in vivo. For in vitro studies, cell proliferation, alkaline phosphatase activity, and osteoblastic differentiation marker genes were assessed after MC3T3-E1 mouse preosteoblasts were cultured with CMs containing factors. In vivo effects were investigated by placement of CMs containing SDF-1 or PDGF using a rat mandibular bone defect model. At 4 weeks after the surgery, the new bone formation was measured using micro-computed tomography (µCT) and histological analysis. The results of in vitro studies revealed that CM delivery of SDF-1 significantly induced cell proliferation, ALP activity, and gene expression of all osteogenic markers compared to the CM alone or control, similar to PDGF. Quantitative and qualitative µCT analysis for volume of new bone formation and the percentage of new bone area showed that SDF-1-treated groups significantly increased and accelerated bone regeneration compared to control and CM alone. The enhancement of bone formation in SDF-1-treated animals was dose-dependent and with levels similar to those measured with PDGF. These results suggest that a CM with SDF-1 may be a great candidate for growth factor delivery that could be a substitute for PDGF in clinical procedures where bone regeneration is necessary.

Guided Bone Regeneration, Platelet Rich Plasma and Low-Intensity Ultrasound Irradiation for Dental Implants

2006 ◽  
Vol 49 ◽  
pp. 171-180 ◽  
Author(s):  
Kuniteru Nagahara ◽  
Toshiichiro Tanabe ◽  
Noriaki Ito ◽  
Motohiko Nagayama ◽  
Kota Takagi ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Dental Implant ◽  
Platelet Rich Plasma ◽  
Guided Bone Regeneration ◽  
Autogenous Bone ◽  
Bone Block ◽  
Bone Augmentation ◽  
Basic Study ◽  
Low Intensity

Because a concept of an osteointegrated dental implant system was established, prognosis of a dental implant at treatment improved and the treatment of restoration for missing teeth was changed. However, a dental implant treatment into atrophic jaw bone requires bone augmentation, obviously. At this time, we studied for the purpose of establishing the evidence of each method for clinical application of these bone augmentation method, such as guided bone regeneration (GBR), and autogenous bone block graft (BBG). In addition, we pursued the basic study of the evidence about the bone formation with platelet rich plasma (PRP) which recognized the availability in clinic. Furthermore, we present the results of basic studies which we tested for the purpose of applying a low-intensity pulse ultrasound (LIPU) irradiation applied to a fracture treatment in orthopedics area to intra-oral area, specially the condition after implant placement. In the results of comparison with GBR site and BBG, the differences of labeling bands were observed with a fluorescence microscopy. There was much labeling bands on GBR sections in comparison with BBG. This meaning that the bone remodeling around implants at GBR site was more active than BBG site. And the new bone formation by PRP was identified on soft X-ray graphically at first week after PRP applied mandible bone defect (experimental side). At same region of first week specimen, we confirmed positive reactions of platelet derived growth factor

scholarly journals Autogenous bone particles combined with platelet‑rich plasma can stimulate bone regeneration in rabbits

2020 ◽  
Vol 20 (6) ◽  
pp. 1-1
Author(s):  
Huanxin Xie ◽  
Lei Cao ◽  
Linlin Ye ◽  
Jubao Du ◽  
Guixiang Shan ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Platelet Rich Plasma ◽  
Autogenous Bone ◽  
Bone Particles

Stromal Cell-Derived Factor 1 Promotes Cell Migration to Enhance Bone Regeneration After Hypoxic Preconditioning

2019 ◽  
Vol 25 (17-18) ◽  
pp. 1300-1309 ◽  
Author(s):  
Jiaqi Huang ◽  
Hui Chi ◽  
Haiyang Chi ◽  
Lirong Qiu ◽  
Yufu Wang ◽  
...  
Keyword(s):  
Cell Migration ◽  
Bone Regeneration ◽  
Stromal Cell ◽  
Hypoxic Preconditioning ◽  
Stromal Cell Derived Factor

Bioglass Associated With Leukocyte-Poor Platelet-Rich Plasma in the Rabbit Maxillary Sinus: Histomorphometric, Densitometric, and Fractal Analysis

2010 ◽  
Vol 36 (5) ◽  
pp. 333-343 ◽  
Author(s):  
Ivy Kiemle Trindade-Suedam ◽  
Juliana Aparecida Najarro Dearo de Morais ◽  
Rafael Silveira Faeda ◽  
Fábio Renato Manzolli Leite ◽  
Guilherme Monteiro Tosoni ◽  
...  
Keyword(s):  
Fractal Dimension ◽  
Bone Density ◽  
Bone Formation ◽  
Maxillary Sinus ◽  
Platelet Rich Plasma ◽  
Autogenous Bone ◽  
New Bone Formation ◽  
Mean Values ◽  
Dimension Analysis ◽  
Fractal Dimension Analysis

Abstract The objective of the present study was to evaluate the outcomes of autogenous bone graft (AB) and bioglass (BG) associated or not with leukocyte-poor platelet-rich plasma (LP-PRP) in the rabbit maxillary sinus (MS) by histomorphometric and radiographic analysis. Twenty rabbits divided into 2 groups (G1, G2) were submitted to sinus lift surgery. In G1, 10 MS were grafted with AB and 10 MS were grafted with BG. In G2, 10 MS were grafted with AB + LP-PRP and 10 MS were grafted with BG + LP-PRP. After 90 days, the animals were killed and specimens were obtained, x-rayed, and submitted to histomorphometric, radiographic bone density (RD) and fractal dimension analysis. Radiographic bone density mean values (SD), expressed as aluminum equivalent in mm, of AB, BG, AB + LP-PRP, and BG + LP-PRP groups were 1.79 (0.31), 2.04 (0.39), 1.61 (0.28), and 1.53 (0.30), respectively. Significant differences (P &lt; 0.05) were observed between BG and AB, and BG + PRP and BG. Fractal dimension mean values were 1.48 (0.04), 1.35 (0.08), 1.44 (0.04), and 1.44 (0.06), respectively. Significant differences were observed between BG and AB, and AB + LP-PRP and BG. Mean values for the percentage of bone inside MS were 63.30 (8.60), 52.65 (10.41), 55.25 (7.01), and 51.07 (10.25), respectively. No differences were found. No correlations were observed among percentage of bone, RD and FD. Histological analysis showed that MS treated with AB presented mature and new bone formation. The other groups showed minor bone formation. Within the limitations of this study, the results indicated that at a 90-day time end point, AB yielded better results than AB + LP-PRP, BG, and BG + LP-PRP and should be considered the primary material for MS augmentation.

Stromal cell-derived factor-1 accelerates bone regeneration through multiple regenerative mechanisms

2019 ◽  
Vol 31 (4) ◽  
pp. 245-250 ◽  
Author(s):  
Yuji Ando ◽  
Jun Ishikawa ◽  
Masahito Fujio ◽  
Yoshihiro Matsushita ◽  
Hirotaka Wakayama ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Stromal Cell ◽  
Stromal Cell Derived Factor

scholarly journals Mesenchymal stem cell expression of stromal cell-derived factor-1β augments bone formation in a model of local regenerative therapy

2014 ◽  
Vol 33 (2) ◽  
pp. 174-184 ◽  
Author(s):  
Samuel Herberg ◽  
Galina Kondrikova ◽  
Khaled A. Hussein ◽  
Maribeth H. Johnson ◽  
Mohammed E. Elsalanty ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Formation ◽  
Stromal Cell ◽  
Regenerative Therapy ◽  
Stromal Cell Derived Factor ◽  
Cell Expression
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