scholarly journals Determination of the second virial coefficient of bovine serum albumin under varying pH and ionic strength by composition-gradient multi-angle static light scattering

2014 ◽  
Vol 41 (1) ◽  
pp. 85-97 ◽  
Author(s):  
Yingfang Ma ◽  
Diana M. Acosta ◽  
Jon R. Whitney ◽  
Rudolf Podgornik ◽  
Nicole F. Steinmetz ◽  
...  
Keyword(s):  
Light Scattering ◽  
Ionic Strength ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Virial Coefficient ◽  
Second Virial Coefficient ◽  
Static Light Scattering ◽  
Composition Gradient

Determination of tannic acid after precipitation with bovine serum albumin by visible light scattering in a flow injection system

2010 ◽  
Vol 169 (1-2) ◽  
pp. 117-122 ◽  
Author(s):  
Tzong-Jih Cheng ◽  
Hsien-Yi Hsiao ◽  
Chien-Yu Chung ◽  
Po-Chung Chen ◽  
Richie L. C. Chen
Keyword(s):  
Light Scattering ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Visible Light ◽  
Tannic Acid ◽  
Flow Injection ◽  
Bovine Serum ◽  
Injection System ◽  
Flow Injection System

scholarly journals The origin and consequences of concentration dependence in gel chromatography

1973 ◽  
Vol 133 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Lawrence W. Nichol ◽  
Masanobu Janado ◽  
Donald J. Winzor
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Concentration Dependence ◽  
Bovine Serum ◽  
Virial Coefficient ◽  
Second Virial Coefficient ◽  
Gel Chromatography ◽  
Elution Volume ◽  
Sodium Phosphate Buffer ◽  
Osmotic Effects

Concentration dependence of elution volume was determined for Blue Dextran 2000, Dextran 500, Dextran sulphate 500 and bovine serum albumin on columns of Sephadex G-100 equilibrated with sodium phosphate buffer, I 0.1, pH6.8. From the results for Dextran 500, it was shown that a linear relation exists between elution volume and the corresponding osmotic pressure calculated for the same concentration and incorporating the term containing the second virial coefficient. This relationship was used to predict the concentration dependence of elution volume for bovine serum albumin and myoglobin, proteins that partially penetrate Sephadex G-100. Possible consequences of osmotic effects are considered in relation to various types of column experiments, including differential chromatography.

scholarly journals Electrochemical characterization and determination of carbamazepine as pharmaceutical standard and tablet content on gold electrode

2014 ◽  
Vol 68 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Nemanja Trisovic ◽  
Bojan Bozic ◽  
Slobodan Petrovic ◽  
Svetlana Tadic ◽  
Milka Avramov-Ivic
Keyword(s):  
Cyclic Voltammetry ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Phosphate Buffer ◽  
Bovine Serum ◽  
Gold Electrode ◽  
Anticonvulsant Drug ◽  
Voltammetric Techniques ◽  
Anodic Behaviour

The anodic behaviour of carbamazepine (CBZ), an anticonvulsant drug, has been studied on gold electrode in 0.1 mol dm-3 phosphate buffer of pH 7.0 by using cyclic voltammetry. It has been found that the value of the oxidative current of pure CBZ at +0.90 V is a linear function of the concentration in a range from 1.0?10-7 to 1.0?10?4 mol dm?3. The detection of CBZ in the concentration of 1.0?10-8 mol dm-3 is among the lowest that have been reported for this drug using voltammetric techniques. CBZ as a content of tablet Galepsine? has been quantitatively determined. It has also been demonstrated that the modification of gold electrode with bovine serum albumin (BSA) results in a decrease of the oxidative peak current due to the binding of the drug to BSA.

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