A Split Enhanced Green Fluorescent Protein-Based Reporter in Yeast Two-Hybrid System

2007 ◽  
Vol 26 (2) ◽  
pp. 107-116 ◽  
Author(s):  
Kyoungsook Park ◽  
So Yeon Yi ◽  
Chang-Soo Lee ◽  
Kyoon Eon Kim ◽  
Hyun-Sook Pai ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Hybrid System ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Green Fluorescent ◽  
Two Hybrid

scholarly journals Detection of Protein-Protein Interactions Using a Green Fluorescent Protein-Based Mammalian Two-Hybrid System

BioTechniques ◽  
2000 ◽  
Vol 29 (1) ◽  
pp. 22-26 ◽  
Author(s):  
M. Fotin-Mleczek ◽  
M. Rottmann ◽  
G. Rehg ◽  
S. Rupp ◽  
F.-J. Johannes
Keyword(s):  
Green Fluorescent Protein ◽  
Hybrid System ◽  
Protein Interactions ◽  
Fluorescent Protein ◽  
Protein Protein Interactions ◽  
Green Fluorescent ◽  
Two Hybrid

Identifications of DNA Sequences Encoding Key Region of SCR Interacting with SRK Extracellular Domain by Using Yeast Two-Hybrid System

2013 ◽  
Vol 38 (9) ◽  
pp. 1583-1591
Author(s):  
Li-Yan XUE ◽  
Bing LUO ◽  
Li-Quan ZHU ◽  
Yong-Jun YANG ◽  
He-Cui ZHANG ◽  
...  
Keyword(s):  
Hybrid System ◽  
Dna Sequences ◽  
Extracellular Domain ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Two Hybrid

scholarly journals Enhanced Green Fluorescent Protein (GFP) fluorescence after polyelectrolyte caging

2006 ◽  
Vol 14 (21) ◽  
pp. 9815 ◽  
Author(s):  
Alberto Diaspro ◽  
Silke Krol ◽  
Barbara Campanini ◽  
Fabio Cannone ◽  
Giuseppe Chirico
Keyword(s):  
Green Fluorescent Protein ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Gfp Fluorescence ◽  
Green Fluorescent

scholarly journals Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b’ Is Not Required for In Vitro Virus Replication

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 632
Author(s):  
Yingyun Cai ◽  
Shuiqing Yu ◽  
Ying Fang ◽  
Laura Bollinger ◽  
Yanhua Li ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Cell Lines ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Infectious Clone ◽  
Hemorrhagic Fever ◽  
Simian Hemorrhagic Fever Virus ◽  
Hemorrhagic Fever Virus ◽  
Green Fluorescent

Simian hemorrhagic fever virus (SHFV) causes acute, lethal disease in macaques. We developed a single-plasmid cDNA-launch infectious clone of SHFV (rSHFV) and modified the clone to rescue an enhanced green fluorescent protein-expressing rSHFV-eGFP that can be used for rapid and quantitative detection of infection. SHFV has a narrow cell tropism in vitro, with only the grivet MA-104 cell line and a few other grivet cell lines being susceptible to virion entry and permissive to infection. Using rSHFV-eGFP, we demonstrate that one cricetid rodent cell line and three ape cell lines also fully support SHFV replication, whereas 55 human cell lines, 11 bat cell lines, and three rodent cells do not. Interestingly, some human and other mammalian cell lines apparently resistant to SHFV infection are permissive after transfection with the rSHFV-eGFP cDNA-launch plasmid. To further demonstrate the investigative potential of the infectious clone system, we introduced stop codons into eight viral open reading frames (ORFs). This approach suggested that at least one ORF, ORF 2b’, is dispensable for SHFV in vitro replication. Our proof-of-principle experiments indicated that rSHFV-eGFP is a useful tool for illuminating the understudied molecular biology of SHFV.

Screening of binding proteins that interact with human Salvador 1 in a human fetal liver cDNA library by the yeast two-hybrid system

2012 ◽  
Vol 39 (8) ◽  
pp. 8225-8230 ◽  
Author(s):  
Xiaolan Li ◽  
Xuelai Luo ◽  
Zhaoming Li ◽  
Guihua Wang ◽  
Hui Xiao ◽  
...  
Keyword(s):  
Cdna Library ◽  
Hybrid System ◽  
Binding Proteins ◽  
Fetal Liver ◽  
Yeast Two Hybrid ◽  
Human Fetal Liver ◽  
Yeast Two Hybrid System ◽  
Liver Cdna Library ◽  
Two Hybrid

Identification of MAL2, a Novel Member of the MAL Proteolipid Family, Though Interactions with TPD52-like Proteins in the Yeast Two-Hybrid System

Genomics ◽  
2001 ◽  
Vol 76 (1-3) ◽  
pp. 81-88 ◽  
Author(s):  
Sarah H.D Wilson ◽  
Angela M Bailey ◽  
Craig R Nourse ◽  
Marie-Geneviève Mattei ◽  
Jennifer A Byrne
Keyword(s):  
Hybrid System ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Two Hybrid

scholarly journals Interactions among members of the Bcl-2 protein family analyzed with a yeast two-hybrid system.

1994 ◽  
Vol 91 (20) ◽  
pp. 9238-9242 ◽  
Author(s):  
T. Sato ◽  
M. Hanada ◽  
S. Bodrug ◽  
S. Irie ◽  
N. Iwama ◽  
...  
Keyword(s):  
Hybrid System ◽  
Protein Family ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Two Hybrid
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