scholarly journals The use of cotton blue stain to improve the efficiency of picking and identifying chironomid head capsules

2010 ◽  
Vol 45 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Isabelle Larocque-Tobler ◽  
Florencia Oberli
Keyword(s):  
Cotton Blue ◽  
Blue Stain

Evaluation of Lacto-Phenol Cotton Blue Stain for Detection of Eggs ofEnterobius Vermicularisin Perianal Surface Samples

2001 ◽  
Vol 31 (4) ◽  
pp. 214-215 ◽  
Author(s):  
S C Parija ◽  
C Sheeladevi ◽  
M R Shivaprakash ◽  
N Biswal
Keyword(s):  
Surface Samples ◽  
Cotton Blue ◽  
Blue Stain

scholarly journals Isolation and identification of some pathogenic species of Fungi from Shatt-al-Arab River

2021 ◽  
Vol 2063 (1) ◽  
pp. 012021
Author(s):  
Shrouk Abdulrazak Hassan Al-Ibraheem ◽  
Angham O S Al-Zeadei
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Room Temperature ◽  
Pathogenic Fungi ◽  
Pathogenic Species ◽  
Isolation And Identification ◽  
Cotton Blue ◽  
Blue Stain ◽  
The Media ◽  
Aspergillus Candidus

Abstract This study aimed to isolation and identification of pathogenic fungi from Shatt – al-Arab River in Basra city, Fourteen water samples were collected from different area from Shatt-al-Arab River (AL Ashar, AL Tnoma, AL Makal, AL Qurna, AL Karma, AL Jabiluh, AL-Hartha), from October to December in 2017, with 250 ml volume, this samples centrifuged at 5000 rpm for 10 min at room temperature, the floating was removed and then take the precipitate and pour directly into the center of the media of SDA and PDA and then incubation in a temperature range25-27c for 4 days after that the growth on the media made pure culture and each fungi species diagnosed based on the cultural and microbiological phenotypes, smear prepared with lacto phenol cotton blue stain and the results show 57.1% of growth was Aspergillus niger, 85.7% Aspergillus flavus and 42.8% was Aspergillus candidus and 14.2% was Rhizopus, while the results show 42.8% of growth was Penicillium..

Semipermanent microscope slides of microfungi using a sticky tape technique

1980 ◽  
Vol 26 (4) ◽  
pp. 551-553 ◽  
Author(s):  
T. W. Flegel
Keyword(s):  
Ethyl Alcohol ◽  
Microscope Slide ◽  
Cover Glass ◽  
Cotton Blue ◽  
Blue Stain ◽  
Sticky Tape ◽  
Microscope Slides ◽  
Fungal Colony ◽  
Glycerine Solution

A technique is described for making semipermanent microscope slides of fungi using sticky tape. After being touched to a fungal colony, a modified segment of sticky tape is touched to ethyl alcohol and then immersed in a 50% glycerine solution containing cotton blue stain. Finally, it is transferred (sticky side up) to a microscope slide, covered with a cover glass, and sealed.

scholarly journals A single method to stain Malassezia furfur and Corynebacterium minutissimum in scales

1996 ◽  
Vol 38 (4) ◽  
pp. 299-302 ◽  
Author(s):  
Antar Padilha-Gonçalves
Keyword(s):  
Microscopic Examination ◽  
Practical Method ◽  
Malassezia Furfur ◽  
Absolute Alcohol ◽  
Scotch Tape ◽  
Cotton Blue ◽  
Blue Stain ◽  
Current Water ◽  
Single Method ◽  
Platinum Loop

A single and practical method to slain Malassezia furfur and Corynebacterium minutissimum in lesions' scales is described. The scales are collected by pressing small pieces of scotch tape (about 4 cm lenght and 2 cm width) onto the lesions and following withdrawl the furfuraceous scales will remain on the glue side. These pieces are then immersed for some minutes in lactophenol-cotton blue stain. Following absorption of the stain the scales are washed in current water to remove the excess of blue stain, dried with filter paper, dehydrated via passage in two bottles containing absolute alcohol and then placed in xylene in a centrifugation tube. The xylene dissolves the scotch tape glue and the scales fall free in the tube. After centrifugation and decantation the scales concentrated on the bottom of the tube are collected with a platinum-loop, placed in Canada balsam on a microscopy slide and closed with a cover slip. The preparations are then ready to be submitted to microscopic examination. Other stains may also be used instead of lactophenol-cotton blue. This method is simple, easily performed, and offers good conditions to study these fungi as well as being useful for the diagnosis of the diseases that they cause.

Lacto-Phenol Cotton Blue Stain for the Wet Mount Preparation of Hydatid Fluid

1997 ◽  
Vol 27 (2) ◽  
pp. 108-108 ◽  
Author(s):  
S C Parija ◽  
P T Ravinder
Keyword(s):  
Hydatid Fluid ◽  
Wet Mount ◽  
Cotton Blue ◽  
Blue Stain

scholarly journals Endemic Jeffrey Pine Beetle Associates: Beetle/Mite Fungal Dissemination Strategies and Interactions That May Influence Beetle Population Levels

2021 ◽  
Vol 9 (8) ◽  
pp. 1641
Author(s):  
Javier E. Mercado ◽  
Beatriz Ortiz-Santana ◽  
Shannon L. Kay
Keyword(s):  
Bark Beetle ◽  
Mountain Pine Beetle ◽  
Jeffrey Pine ◽  
Mutualistic Interaction ◽  
Pine Beetle ◽  
Blue Stain Fungi ◽  
Blue Stain ◽  
Grosmannia Clavigera ◽  
Mutualistic Fungus ◽  
Jeffrey Pine Beetle

Fungal and mite associates may drive changes in bark beetle populations, and mechanisms constraining beetle irruptions may be hidden in endemic populations. We characterized common fungi of endemic-level Jeffrey pine beetle (JPB) in western USA and analyzed their dissemination by JPB (maxillae and fecal pellet) and fungivorous mites to identify if endogenous regulation drove the population. We hypothesized that: (1) as in near-endemic mountain pine beetle populations, JPB’s mutualistic fungus would either be less abundant in endemic than in non-endemic populations or that another fungus may be more prevalent; (2) JPB primarily transports its mutualistic fungus, while its fungivorous mites primarily transport another fungus, and (3) based on the prevalence of yeasts in bark beetle symbioses, that a mutualistic interaction with blue-stain fungi present in that system may exist. Grosmannia clavigera was the most frequent JPB symbiont; however, the new here reported antagonist, Ophiostoma minus, was second in frequency. As hypothesized, JPB mostly carried its mutualist fungus while another fungus (i.e., antagonistic) was mainly carried by mites, but no fungal transport was obligate. Furthermore, we found a novel mutualistic interaction between the yeast Kuraishia molischiana and G. clavigera which fostered a growth advantage at temperatures associated with beetle colonization.

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