Lacto-Phenol Cotton Blue Stain for the Wet Mount Preparation of Hydatid Fluid

1997 ◽  
Vol 27 (2) ◽  
pp. 108-108 ◽  
Author(s):  
S C Parija ◽  
P T Ravinder
Keyword(s):  
Hydatid Fluid ◽  
Wet Mount ◽  
Cotton Blue ◽  
Blue Stain

scholarly journals The use of cotton blue stain to improve the efficiency of picking and identifying chironomid head capsules

2010 ◽  
Vol 45 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Isabelle Larocque-Tobler ◽  
Florencia Oberli
Keyword(s):  
Cotton Blue ◽  
Blue Stain

scholarly journals Mixed dermatophytic infections: a learning perspective and report of ten cases

2019 ◽  
Vol 6 (1) ◽  
pp. 174
Author(s):  
Pooja Singla ◽  
Priyadarshini Sahu ◽  
Pratibha Mane ◽  
Prakriti Vohra
Keyword(s):  
Tinea Capitis ◽  
Fungal Infections ◽  
Pathogenic Fungi ◽  
Body Site ◽  
Rare Entity ◽  
Sample Collection ◽  
Tinea Cruris ◽  
Wet Mount ◽  
Hair Samples ◽  
Cotton Blue

Background: Isolation of two or more than two pathogenic fungi from the same body site in a patient is considered as a rare entity and very few cases have been reported in literature. These types of infections are called as mixed/ combined fungal infections. Author are enumerating ten cases of superficial mycoses in which two different dermatophytes were grown from the same focus.Methods: From clinically suspected cases of dermatophytosis, skin and hair samples were collected from the affected sites and examined by standard mycological procedures. Microscopy was done by using 10% KOH wet mount. Culture was put on Sabouraud’s dextrose agar with cyclohexamide medium. Growth was identified by lactophenol cotton blue mount.Results: Mixed dermatophytes were obtained from tinea corporis (five cases), tinea capitis (four cases) and tinea cruris (one case) patients. Fungal combinations from given cases involved two different species of genus Trichophyton which were as follows: T. violaceum+T. tonsurans, T. verrucosum+T. tonsurans, T. violaceum (violet) and T. violaceum (white), T. mentagrophytes+T. Violaceum, T. rubrum+T. tonsurans, T. violaceum+T. rubrum, T. rubrum+T. mentagrophytes, T. verrucosum+T. mentagrophytes, T. mentagrophytes+T. tonsurans, Malassezia+T. mentagrophytes.Conclusions: Inspite of the frequent occurrence of dermatophytic infections worldwide, reports on mixed dermatophytes are very few. With proper sample collection and proper identification procedures, more cases can be identified and added to the existing literature.

Evaluation of Lacto-Phenol Cotton Blue Stain for Detection of Eggs ofEnterobius Vermicularisin Perianal Surface Samples

2001 ◽  
Vol 31 (4) ◽  
pp. 214-215 ◽  
Author(s):  
S C Parija ◽  
C Sheeladevi ◽  
M R Shivaprakash ◽  
N Biswal
Keyword(s):  
Surface Samples ◽  
Cotton Blue ◽  
Blue Stain

Thick Stool Smear Wet Mount Examination: A New Approach in Stool Microscopy

2003 ◽  
Vol 33 (3) ◽  
pp. 173-173 ◽  
Author(s):  
Subhash Chandra Parija ◽  
Sanjay Bhattacharya ◽  
Prasant Padhan
Keyword(s):  
Intestinal Parasites ◽  
Laboratory Diagnosis ◽  
Parasitic Infections ◽  
New Approach ◽  
Intestinal Parasitic Infections ◽  
Wet Mount ◽  
Cotton Blue ◽  
Novel Method ◽  
Stool Samples ◽  
The Difference

Microscopic examination of a wet mount of the stool has been the standard practice for the laboratory diagnosis of intestinal parasitic infections. Here we describe a novel method of stool microscopy of 80 stool samples, 31 (38.75%) were positive by the new thick stool smear wet mount method, whereas the corresponding figure for the conventional method using lacto-phenol cotton blue was 16 (20%). The difference was found to be statistically significant ( P<0.04 by McNemar's test). The thick stool smear wet mount procedure promises to be superior to the direct wet mount method in the detection of the intestinal parasites.

scholarly journals Keratitis due toChaetomiumsp.

2011 ◽  
Vol 2011 ◽  
pp. 1-3 ◽  
Author(s):  
Jayaraman Kaliamurthy ◽  
Catti Munuswamy Kalavathy ◽  
Christadoss Arul Nelson Jesudasan ◽  
Philip A. Thomas
Keyword(s):  
Corneal Ulcer ◽  
Culture Media ◽  
Aerial Mycelium ◽  
Hand Movements ◽  
Wet Mount ◽  
Greenish Yellow ◽  
Ocular Lesions ◽  
Cotton Blue ◽  
History Of ◽  
The Right

Aim. To describe keratitis due toChaetomiumsp. occurring in a 65-year-old woman who presented with a corneal ulcer with hypopyon of the right eye with a history of trauma by vegetable matter.Method. Multiple scrapings were obtained from the ulcer. A lactophenol cotton blue wet mount and a Gram-stained smear of the scrapings were made. Scrapings were also inoculated onto various culture media.Results. Direct microscopy of corneal scrapings revealed moderate numbers of septate fungal hyphae. Greenish-yellow-coloured fungal colonies with aerial mycelium were observed in culture of the corneal scrapes. On the basis of colony characteristics and conidial structure, the fungal isolate was identified asChaetomiumsp. The patient was treated with topical natamycin (5%) hourly and cyclopentolate 1% drops 3 times a day. After 4 weeks of therapy, the hypopyon had disappeared, the epithelial defect had healed, and the stromal infiltration had almost completely resolved; the visual acuity of the eye improved from hand movements to(1/2)/60.Conclusion. Fungi of the genusChaetomium, which are rare causes of human disease (systemic mycosis, endocarditis, subcutaneous lesions), may also cause ocular lesions.

scholarly journals Isolation and identification of some pathogenic species of Fungi from Shatt-al-Arab River

2021 ◽  
Vol 2063 (1) ◽  
pp. 012021
Author(s):  
Shrouk Abdulrazak Hassan Al-Ibraheem ◽  
Angham O S Al-Zeadei
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Room Temperature ◽  
Pathogenic Fungi ◽  
Pathogenic Species ◽  
Isolation And Identification ◽  
Cotton Blue ◽  
Blue Stain ◽  
The Media ◽  
Aspergillus Candidus

Abstract This study aimed to isolation and identification of pathogenic fungi from Shatt – al-Arab River in Basra city, Fourteen water samples were collected from different area from Shatt-al-Arab River (AL Ashar, AL Tnoma, AL Makal, AL Qurna, AL Karma, AL Jabiluh, AL-Hartha), from October to December in 2017, with 250 ml volume, this samples centrifuged at 5000 rpm for 10 min at room temperature, the floating was removed and then take the precipitate and pour directly into the center of the media of SDA and PDA and then incubation in a temperature range25-27c for 4 days after that the growth on the media made pure culture and each fungi species diagnosed based on the cultural and microbiological phenotypes, smear prepared with lacto phenol cotton blue stain and the results show 57.1% of growth was Aspergillus niger, 85.7% Aspergillus flavus and 42.8% was Aspergillus candidus and 14.2% was Rhizopus, while the results show 42.8% of growth was Penicillium..

Evaluation of Chicago sky blue stain against Potassium Hydroxide-Dimethyl Sulfoxide wet mount in the identification of dermatophytes

2020 ◽  
Vol 14 (2) ◽  
pp. 83-86
Author(s):  
Majid Rauf Ahmad ◽  
Iffat Javed ◽  
Suhaila Mushtaq
Keyword(s):  
Dimethyl Sulfoxide ◽  
Potassium Hydroxide ◽  
Tertiary Care ◽  
Blue Staining ◽  
Medical Institute ◽  
Color Contrast ◽  
Care Hospital ◽  
Direct Microscopy ◽  
Wet Mount ◽  
Blue Stain

Background: Dermatophytoses infections are widespread in the developing world. The laboratory diagnosis of dermatophytes has been a challenge as it involves microscopy and trained personnel. Potassium hydroxide wet mount with dimethyl sulfoxide added is routinely used in direct microscopy. But it lacks color contrast and the hyphae may be missed on routine microscopy. The study aimed to evaluate the effectiveness of the Chicago sky blue stain against routine potassium hydroxide-dimethyl sulfoxide (KOH/DMSO) wet mount in direct microscopy. Patients and methods: The study was carried out at the Department of Microbiology, Postgraduate Medical Institute, Lahore over a period of nine months from July 2013 till March 2014. Patients of either gender regardless of age, clinically diagnosed as having dermatophytoses by dermatologists were selected for this study. Specimens from 100 patients were collected from the dermatology outdoor of a tertiary care hospital for this study. They were evaluated microscopically with routine potassium hydroxide-dimethyl sulfoxide (KOH-DMSO) wet mount and Chicago sky blue (CSB) stain. Data were collected and entered in Statistical Package for the Social Sciences (SPSS) version 20.0. Results: Out of a total of 100 samples collected from skin, hair and nails, 59% were positive on direct microscopy with KOH/DMSO wet mount. Whereas direct microscopy using CSB stain revealed dermatophytes in 62% of cases. Conclusion: Chicago sky blue staining is a better technique for the detection of dermatophytes as compared to potassium hydroxide wet mount examination. It is simple, rapid, and easy to interpret. We recommend the use of this technique to improve the detection of dermatophytes without awaiting the results of the culture.

Evaluation of Formalin — Acetone Sedimentation in the Concentration of Stool for Intestinal Parasites

2003 ◽  
Vol 33 (3) ◽  
pp. 163-164 ◽  
Author(s):  
S C Parija ◽  
S Bhattacharya ◽  
P Padhan ◽  
M R Shivaprakash
Keyword(s):  
Recovery Rate ◽  
Intestinal Parasites ◽  
Wet Mount ◽  
Significant Difference ◽  
Cotton Blue ◽  
Stool Specimens

Formalin-acetone sedimentation was compared with the formalin-ether method for the concentration of stool for intestinal parasites. Of 80 stool specimens, 45 (56.25%) were positive for parasites by the formalin-acetone method. The figures for the two methods were formalin-ether 35 (43.75%) and for the direct lacto-phenol cotton blue wet mount method 17 (21.25%). There was no statistically significant difference in the parasite recovery rate between the two methods. Acetone is more stable, safer, and a cheaper fat solvent and promises to be a useful alternative to ether.

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