A Standardized Cotton Blue Stain for Pollen Germination and Growth in Andropogoneae Grasses

1971 ◽  
Vol 46 (5) ◽  
pp. 239-243 ◽  
Author(s):  
U. R. Murty
2010 ◽  
Vol 45 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Isabelle Larocque-Tobler ◽  
Florencia Oberli
Keyword(s):  

2001 ◽  
Vol 31 (4) ◽  
pp. 214-215 ◽  
Author(s):  
S C Parija ◽  
C Sheeladevi ◽  
M R Shivaprakash ◽  
N Biswal

1961 ◽  
Vol 41 (4) ◽  
pp. 740-743 ◽  
Author(s):  
G. W. Eaton

Effects of fungicidal concentrations of sulphur, dichlone, ferbam, and captan on sweet cherry (Prunus avium L.) pollen germination and growth were studied in artificial media. Sulphur did not reduce the germination of pollen. Dichlone and ferbam reduced germination from 53.0 per cent to 47.1 per cent and 40 per cent, respectively, reductions which were significant statistically. Sprayed at 0.2 pound/100 gallons or less, captan did not reduce germination. Captan, sprayed at 2 pounds/100 gallons, almost entirely prevented pollen germination, and arrested the elongation of pollen tubes.


2004 ◽  
Vol 45 (10) ◽  
pp. 1519-1528 ◽  
Author(s):  
Min-Long Wang ◽  
Chia-Mei Hsu ◽  
Liang-Chi Chang ◽  
Co-Shine Wang ◽  
Ting-Ho Su ◽  
...  

1966 ◽  
Vol 6 (23) ◽  
pp. 371 ◽  
Author(s):  
DE Byth

Anthesis insoybeans was found to occur at between 7.00 a.m. and 8.00 a.m. in south-eastem Queensland. A successful hybridization technique involving high relative humidity is described. The effects of humidity were involved with stigmatic receptivity, rather than with limitations on pollen germination and growth under low relative humidities. Greater pollen germination was obtained at 26.5�C than at 21�C, but germination was suppressed at 33�C. Applications of gibberellic acid stimulated pollen grain germination, and a dual application of gibberellic acid and certain growth substances was slightly more effective than gibberellic acid alone. Individual growth substances had little influence on pollen grain germination.


1991 ◽  
Vol 69 (9) ◽  
pp. 2033-2043 ◽  
Author(s):  
Pamela K. Diggle

Comparison of hermaphrodite and staminate flowers of andromonoecious Solanum hirtum must consider two sources of morphological variation: variation due to differences in flower position and variation due to conditions that affect sex expression. In S. hirtum, positional variation occurs among hermaphrodite flowers of unpollinated plants; flowers vary quantitatively but not functionally with position on the inflorescence. On inflorescences of pollinated, fruit-bearing plants, flowers vary both quantitatively and functionally with position such that flowers borne distally are functionally staminate. Corolla, anther, style, and ovary length of staminate flowers are significantly smaller than those of hermaphrodite flowers and they have fewer, smaller ovules. Failure of fruit set in staminate flowers is due to a combination of factors. Stigmas and styles of staminate flowers are reduced and appear to lack the secretions necessary for normal pollen germination and growth. In the ovules, the integumentary tapetum is collapsed and appears senescent and most megagametophytes are abnormal. Lack of pollen tube growth and abnormal ovules could both contribute to gynoecial sterility in staminate flowers. Key words: andromonoecy, floral structure, reproductive anatomy, sex expression, Solanum.


HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 762B-762
Author(s):  
Vikramjit S. Bajwa* ◽  
Curt Rom

Osmotic agents used to prevent apple pollen grain germination were studied in vitro by applying 10 μL of solutions to germinating apple pollen on germinating and growth media. Seven concentrations (0%, 0.25%, 0.5%, 1%, 2%, 5% and 10%) of the solution were prepared for each chemical and the characteristics of pH, EC, and osmotic potential were measured. Apple pollen was dispersed onto the media in petri dishes. Micro drops of solution were then applied to marked areas. Dishes were then placed in germination cabinets at 25 °C. Cumulative percentage pollen germination was calculated 4, 8, 12, and 24 h after treatment by microscopic observation. Generally, the cumulative percentage pollen germination decreased asymptotically with increasing chemical concentration. The most effective chemicals for restricting pollen germination and growth were CuSO4 (0.25%), CH3 COOH (0.25%), CaCl2 (10%), K2 S2 O5 (0.25%), Methyl Jasmonate (2%). The effect of these chemicals has also been tested on pistil viability both in vitro and on excised limbs.


2021 ◽  
Vol 2063 (1) ◽  
pp. 012021
Author(s):  
Shrouk Abdulrazak Hassan Al-Ibraheem ◽  
Angham O S Al-Zeadei

Abstract This study aimed to isolation and identification of pathogenic fungi from Shatt – al-Arab River in Basra city, Fourteen water samples were collected from different area from Shatt-al-Arab River (AL Ashar, AL Tnoma, AL Makal, AL Qurna, AL Karma, AL Jabiluh, AL-Hartha), from October to December in 2017, with 250 ml volume, this samples centrifuged at 5000 rpm for 10 min at room temperature, the floating was removed and then take the precipitate and pour directly into the center of the media of SDA and PDA and then incubation in a temperature range25-27c for 4 days after that the growth on the media made pure culture and each fungi species diagnosed based on the cultural and microbiological phenotypes, smear prepared with lacto phenol cotton blue stain and the results show 57.1% of growth was Aspergillus niger, 85.7% Aspergillus flavus and 42.8% was Aspergillus candidus and 14.2% was Rhizopus, while the results show 42.8% of growth was Penicillium..


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