Semipermanent microscope slides of microfungi using a sticky tape technique

1980 ◽  
Vol 26 (4) ◽  
pp. 551-553 ◽  
Author(s):  
T. W. Flegel
Keyword(s):  
Ethyl Alcohol ◽  
Microscope Slide ◽  
Cover Glass ◽  
Cotton Blue ◽  
Blue Stain ◽  
Sticky Tape ◽  
Microscope Slides ◽  
Fungal Colony ◽  
Glycerine Solution

A technique is described for making semipermanent microscope slides of fungi using sticky tape. After being touched to a fungal colony, a modified segment of sticky tape is touched to ethyl alcohol and then immersed in a 50% glycerine solution containing cotton blue stain. Finally, it is transferred (sticky side up) to a microscope slide, covered with a cover glass, and sealed.

A SQUASH METHOD FOR SOMATIC CHROMOSOMES OF APHIDS

1957 ◽  
Vol 35 (6) ◽  
pp. 711-713 ◽  
Author(s):  
Leo A. Dionne ◽  
P. B. Spicer
Keyword(s):  
Acetic Acid ◽  
Hydrochloric Acid ◽  
Ethyl Alcohol ◽  
Glacial Acetic Acid ◽  
Absolute Alcohol ◽  
Microscope Slide ◽  
Cover Glass ◽  
Good Source ◽  
Ringer’S Solution

Embryonic aphids are a good source of aphid chromosomes when extruded and left for 15 minutes in 25% Ringer's solution, fixed for 1 hour in a mixture of 2 parts glacial acetic acid, 2 parts 95% ethyl alcohol, and 0.125 parts of chloroform. The embryos are then hydrolyzed for 5 minutes in normal hydrochloric acid at 60 °C. and stained in Gomori's hematoxylin for 30 minutes at 60 °C. After differentiation in 45% acetic acid for [Formula: see text]to 2 hours, the embryos are placed on a microscope slide, squashed under a cover glass, and mounted in 45% acetic acid. Individual plates are spread and flattened at low magnification with the point of a scalpel by tapping and pressing the cover glass over the figure. Unused embryos in 45% acetic acid and prepared slides will keep in "deep freeze" for about two weeks. Permanent preparations are made by floating the cover glasses from frozen slides in cold 95% ethyl alcohol, dehydrating in absolute alcohol, and mounting in an alcohol-based mounting medium.

scholarly journals The use of cotton blue stain to improve the efficiency of picking and identifying chironomid head capsules

2010 ◽  
Vol 45 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Isabelle Larocque-Tobler ◽  
Florencia Oberli
Keyword(s):  
Cotton Blue ◽  
Blue Stain

Evaluation of Lacto-Phenol Cotton Blue Stain for Detection of Eggs ofEnterobius Vermicularisin Perianal Surface Samples

2001 ◽  
Vol 31 (4) ◽  
pp. 214-215 ◽  
Author(s):  
S C Parija ◽  
C Sheeladevi ◽  
M R Shivaprakash ◽  
N Biswal
Keyword(s):  
Surface Samples ◽  
Cotton Blue ◽  
Blue Stain

scholarly journals On certain structures formed in the drying of a fluid with particles in suspension

1898 ◽  
Vol 63 (389-400) ◽  
pp. 217-227
Keyword(s):  
Cover Glass ◽  
Microscope Slides

1. Origin and Method of Experiments . I have frequently had to mount in water, for examination with the microscope, the powder of various rocks. Certain of the slides, when accidentally dried, exhibited rather interesting forms. These I showed to Professor Bonney, who encouraged me to try for farther results, so the experiments were continued with various powdered substances—many pigments (vermilion, indigo, sepia, &c.), chalk and other more or less friable rocks. At first, ordinary microscope slides were used, but afterwards, larger pieces of glass, in each case, generally with a cover-glass placed over the mud. The results seem to be worth describing, as affording familiar, almost homely illustrations which may throw some light on the origin of certain minor structures in rocks.

scholarly journals A Novel Automated Mass Digitisation Workflow for Natural History Microscope Slides

2019 ◽  
Vol 7 ◽  
Author(s):  
E Louise Allan ◽  
Laurence Livermore ◽  
Benjamin Price ◽  
Olha Shchedrina ◽  
Vincent Smith
Keyword(s):  
Natural History ◽  
Human Error ◽  
Microscope Slide ◽  
Unique Identifier ◽  
History Museum ◽  
Comparative Efficiency ◽  
Collection Management System ◽  
Scientific Collections ◽  
Automated Processes ◽  
Microscope Slides

The Natural History Museum, London (NHM) has embarked on an ambitious programme to digitise its collections. One aim of the programme has been to improve the workflows and infrastructure needed to support high-throughput digitisation and create comprehensive digital inventories of large scientific collections. This paper presents the workflow developed to digitise the entire Phthiraptera (parasitic lice) microscope slide collection (70,663 slides). Here we describe a novel process of semi-automated mass digitisation using both temporary and permanent barcode labels applied before and during slide imaging. By using a series of barcodes encoding information associated with each slide (i.e. unique identifier, location in the collection and taxonomic name), we can run a series of automated processes, including file renaming, image processing and bulk import into the NHM’s collection management system. We provide data on the comparative efficiency of these processes, illustrating how simple activities, like automated file renaming, reduces image post-processing time, minimises human error and can be applied across multiple collection types.

scholarly journals Isolation and identification of some pathogenic species of Fungi from Shatt-al-Arab River

2021 ◽  
Vol 2063 (1) ◽  
pp. 012021
Author(s):  
Shrouk Abdulrazak Hassan Al-Ibraheem ◽  
Angham O S Al-Zeadei
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Room Temperature ◽  
Pathogenic Fungi ◽  
Pathogenic Species ◽  
Isolation And Identification ◽  
Cotton Blue ◽  
Blue Stain ◽  
The Media ◽  
Aspergillus Candidus

Abstract This study aimed to isolation and identification of pathogenic fungi from Shatt – al-Arab River in Basra city, Fourteen water samples were collected from different area from Shatt-al-Arab River (AL Ashar, AL Tnoma, AL Makal, AL Qurna, AL Karma, AL Jabiluh, AL-Hartha), from October to December in 2017, with 250 ml volume, this samples centrifuged at 5000 rpm for 10 min at room temperature, the floating was removed and then take the precipitate and pour directly into the center of the media of SDA and PDA and then incubation in a temperature range25-27c for 4 days after that the growth on the media made pure culture and each fungi species diagnosed based on the cultural and microbiological phenotypes, smear prepared with lacto phenol cotton blue stain and the results show 57.1% of growth was Aspergillus niger, 85.7% Aspergillus flavus and 42.8% was Aspergillus candidus and 14.2% was Rhizopus, while the results show 42.8% of growth was Penicillium..

Drying dissipative patterns of dyes in ethyl alcohol on a cover glass

2007 ◽  
Vol 285 (11) ◽  
pp. 1257-1265 ◽  
Author(s):  
Tsuneo Okubo ◽  
Naomi Yokota ◽  
Akira Tsuchida
Keyword(s):  
Ethyl Alcohol ◽  
Cover Glass

Changes in moisture distribution caused by partial reworking of butter shortly after churning

1965 ◽  
Vol 32 (3) ◽  
pp. 263-267 ◽  
Author(s):  
R. M. Dolby
Keyword(s):  
Crystal Structure ◽  
Microscopic Examination ◽  
Moisture Distribution ◽  
Water Droplets ◽  
Microscope Slide ◽  
Cover Glass

SummarySamples of butter were reworked at various times after removal from the churn, either in a laboratory mixer or by pressing between a microscope slide and cover glass. Moisture distribution was studied by microscopic examination and by indicator papers.Reworking within 30 min of completion of the original working caused no change in moisture distribution but delayed reworking caused aggregation of water droplets. The further the setting of the butter had proceeded before reworking, the greater was the extent of aggregation.Cream-cooling treatments which resulted in a softer butter delayed or decreased the aggregation of droplets caused by reworking.A theory is suggested relating changes on reworking with the formation of a crystal structure on setting.

Grossing Technology Today and Tomorrow

2019 ◽  
Vol 51 (4) ◽  
pp. 337-344
Author(s):  
Izak B Dimenstein
Keyword(s):  
Training Program ◽  
Research Evaluation ◽  
Digital Pathology ◽  
Turnaround Time ◽  
Microscope Slide ◽  
High Quality ◽  
New Challenges ◽  
Special Training Program ◽  
Microscope Slides

Abstract Objective To describe the perspective of grossing technology and highlight the prospective of its development in histology laboratory. Methods Analysis of different components of grossing technology. Results Increased requirements for a specimen’s turnaround time and the advancements in modern processing equipment make the triage of workflow a significant part of a grossing person’s responsibilities. The implementation of digital pathology in morphology studies practice requires standardization of fixation, the thickness of gross section, and optimal embedding orientation. To meet tomorrow’s challenges, grossing technology should work on embedding automation and gross digital pathology to record gross sections corresponding the microscope slide. Specialization of grossing stations might be beneficial to the quality of processing and smooth workflow productivity. The emerging grossing technologist subspecialty requires development of a special training program. Conclusion Grossing technology can contribute to new challenges in modern histology laboratory, assuring high-quality microscope slides for the pathologist’s diagnosis and research evaluation.

Sign in / Sign up

Export Citation Format

Share Document