scholarly journals A single method to stain Malassezia furfur and Corynebacterium minutissimum in scales

1996 ◽  
Vol 38 (4) ◽  
pp. 299-302 ◽  
Author(s):  
Antar Padilha-Gonçalves
Keyword(s):  
Microscopic Examination ◽  
Practical Method ◽  
Malassezia Furfur ◽  
Absolute Alcohol ◽  
Scotch Tape ◽  
Cotton Blue ◽  
Blue Stain ◽  
Current Water ◽  
Single Method ◽  
Platinum Loop

A single and practical method to slain Malassezia furfur and Corynebacterium minutissimum in lesions' scales is described. The scales are collected by pressing small pieces of scotch tape (about 4 cm lenght and 2 cm width) onto the lesions and following withdrawl the furfuraceous scales will remain on the glue side. These pieces are then immersed for some minutes in lactophenol-cotton blue stain. Following absorption of the stain the scales are washed in current water to remove the excess of blue stain, dried with filter paper, dehydrated via passage in two bottles containing absolute alcohol and then placed in xylene in a centrifugation tube. The xylene dissolves the scotch tape glue and the scales fall free in the tube. After centrifugation and decantation the scales concentrated on the bottom of the tube are collected with a platinum-loop, placed in Canada balsam on a microscopy slide and closed with a cover slip. The preparations are then ready to be submitted to microscopic examination. Other stains may also be used instead of lactophenol-cotton blue. This method is simple, easily performed, and offers good conditions to study these fungi as well as being useful for the diagnosis of the diseases that they cause.

scholarly journals The use of cotton blue stain to improve the efficiency of picking and identifying chironomid head capsules

2010 ◽  
Vol 45 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Isabelle Larocque-Tobler ◽  
Florencia Oberli
Keyword(s):  
Cotton Blue ◽  
Blue Stain

Evaluation of Lacto-Phenol Cotton Blue Stain for Detection of Eggs ofEnterobius Vermicularisin Perianal Surface Samples

2001 ◽  
Vol 31 (4) ◽  
pp. 214-215 ◽  
Author(s):  
S C Parija ◽  
C Sheeladevi ◽  
M R Shivaprakash ◽  
N Biswal
Keyword(s):  
Surface Samples ◽  
Cotton Blue ◽  
Blue Stain

scholarly journals Recent advances in calculating economic intervention frequency for active leakage control, and implications for calculation of economic leakage levels

2005 ◽  
Vol 5 (6) ◽  
pp. 263-271 ◽  
Author(s):  
A.O. Lambert ◽  
M. Fantozzi
Keyword(s):  
Task Force ◽  
Control Policy ◽  
Practical Method ◽  
Confidence Limits ◽  
Natural Rate ◽  
Water Losses ◽  
Technical Management ◽  
Leakage Control ◽  
Short Run ◽  
Current Water

During the last decade, IWA Water Losses Task Force members have developed a systematic practical approach to the technical management of non-revenue water and its components, with (since 2002) increasing use of 95% confidence limits in these calculations. The current Water Losses Task Force has recently set itself an objective to develop a quick and practical method for calculating economic intervention (for active leakage control to locate unreported leaks and bursts), and short-run economic leakage level. This paper outlines a quick and practical method for assessing economic intervention frequency (and associated budgetary and volumetric parameters) for an active leakage control policy based on regular survey. Calculations are based on three key parameters: natural rate of rise of unreported leakage, marginal cost of water, and cost of intervention. It is hoped this will encourage Utilities that do not currently undertake active leakage control to adopt an ongoing basic active leakage control policy which can be simply demonstrated as being economic for their own situation.

scholarly journals Isolation and identification of some pathogenic species of Fungi from Shatt-al-Arab River

2021 ◽  
Vol 2063 (1) ◽  
pp. 012021
Author(s):  
Shrouk Abdulrazak Hassan Al-Ibraheem ◽  
Angham O S Al-Zeadei
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Room Temperature ◽  
Pathogenic Fungi ◽  
Pathogenic Species ◽  
Isolation And Identification ◽  
Cotton Blue ◽  
Blue Stain ◽  
The Media ◽  
Aspergillus Candidus

Abstract This study aimed to isolation and identification of pathogenic fungi from Shatt – al-Arab River in Basra city, Fourteen water samples were collected from different area from Shatt-al-Arab River (AL Ashar, AL Tnoma, AL Makal, AL Qurna, AL Karma, AL Jabiluh, AL-Hartha), from October to December in 2017, with 250 ml volume, this samples centrifuged at 5000 rpm for 10 min at room temperature, the floating was removed and then take the precipitate and pour directly into the center of the media of SDA and PDA and then incubation in a temperature range25-27c for 4 days after that the growth on the media made pure culture and each fungi species diagnosed based on the cultural and microbiological phenotypes, smear prepared with lacto phenol cotton blue stain and the results show 57.1% of growth was Aspergillus niger, 85.7% Aspergillus flavus and 42.8% was Aspergillus candidus and 14.2% was Rhizopus, while the results show 42.8% of growth was Penicillium..

Semipermanent microscope slides of microfungi using a sticky tape technique

1980 ◽  
Vol 26 (4) ◽  
pp. 551-553 ◽  
Author(s):  
T. W. Flegel
Keyword(s):  
Ethyl Alcohol ◽  
Microscope Slide ◽  
Cover Glass ◽  
Cotton Blue ◽  
Blue Stain ◽  
Sticky Tape ◽  
Microscope Slides ◽  
Fungal Colony ◽  
Glycerine Solution

A technique is described for making semipermanent microscope slides of fungi using sticky tape. After being touched to a fungal colony, a modified segment of sticky tape is touched to ethyl alcohol and then immersed in a 50% glycerine solution containing cotton blue stain. Finally, it is transferred (sticky side up) to a microscope slide, covered with a cover glass, and sealed.

A DIRECT MICROSCOPIC METHOD FOR DETECTING ANTIBIOTIC ACTIVITY IN MILK1

1960 ◽  
Vol 23 (4) ◽  
pp. 117-121 ◽  
Author(s):  
B. J. Liska
Keyword(s):  
Methylene Blue ◽  
Microscopic Examination ◽  
Test Procedure ◽  
Streptococcus Thermophilus ◽  
Antibiotic Activity ◽  
Microscopic Method ◽  
Permanent Record ◽  
Milk Samples ◽  
Blue Stain

A method for detecting antibiotic activity in milk using microscopic examination is presented. The Streptococcus thermophilus culture is exposed to the milk samples for 60–90 min. at 37°C. A methylene blue stain is prepared and examined for changes in morphology or reduction in clump count as compared to a control in antibiotic free milk. Abnormal enlargement or elongation of cells or a 50% reduction in clump count as compared to the control indicates antibiotic activity. Minimum concentrations of various antibiotics detected are: penicillin 0.015 units per ml; bacitracin 0.01 units per ml; terramycin 0.15 micrograms per ml; aureomycin 0.15 micrograms per ml and streptomycin 0.75 micrograms per ml. The test procedure is not affected by normal residues of sanitizers or bacteriophage. The stained milk films can be kept as a permanent record. Leucocycte counts can be made on the stained milk films during microscopic examination.

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