A microcalorimetric method to determine antimicrobial effects of two bile acid derivatives on Staphylococcus aureus

In a previous paper, we showed that bile acid derivatives inhibit capsule formation as well as taurine biosynthesis in a taurine+ (Tau+) encapsulated strain of Staphylococcus aureus. In the present study, binding of [14C]cholic acid ([14C]CA) and [14C]taurocholic acid ([14C]TA) to the staphylococcal polysaccharide antigen (SPA) of the capsular fraction was examined. The bile acids were found to bind with SPA via taurine of the Tau+ cells. [14C]CA bound with the SPA fraction of the Tau+ strain within 10–30 min, whereas 60–120 min was required in the binding of [14C]TA. Various bile acids competed with cholic acid binding to Tau+ cells which was shown by the inhibition of binding with cholic acid or taurocholic acid but not with glycholic acid. Binding of bile acid derivatives to a Tau− encapsulated mutant or to capsular material from this mutant was not observed.

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Comparative study of effects of two bile acid derivatives on Staphylococcus aureus by multiple analytical methods

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2010.03.064 ◽

2010 ◽

Vol 179 (1-3) ◽

pp. 742-747 ◽

Cited By ~ 25

Author(s):

Weijun Kong ◽

Cheng Jin ◽

Xiaohe Xiao ◽

Yanling Zhao ◽

Zulun Li ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Bile Acid ◽

Comparative Study ◽

Analytical Methods ◽

Bile Acid Derivatives

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Effect of bile acid derivatives on taurine biosynthesis and extracellular slime production in encapsulated Staphylococcus aureus S-7.

Infection and Immunity ◽

10.1128/iai.31.2.798-807.1981 ◽

1981 ◽

Vol 31 (2) ◽

pp. 798-807 ◽

Cited By ~ 5

Author(s):

T Ohtomo ◽

K Yoshida ◽

C L San Clemente

Keyword(s):

Staphylococcus Aureus ◽

Bile Acid ◽

Slime Production ◽

Bile Acid Derivatives ◽

Taurine Biosynthesis ◽

Extracellular Slime

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Control of the enantioselectivity by keto bile acid derivatives in the epoxidation of alkenes with Oxone

Tetrahedron Asymmetry ◽

10.1016/j.tetasy.2004.11.012 ◽

2004 ◽

Vol 15 (24) ◽

pp. 3831-3833 ◽

Cited By ~ 13

Author(s):

Olga Bortolini ◽

Giancarlo Fantin ◽

Marco Fogagnolo ◽

Lara Mari

Keyword(s):

Bile Acid ◽

Bile Acid Derivatives ◽

Epoxidation Of Alkenes

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Multiple Antimicrobial Effects of Hybrid Peptides Synthesized Based on the Sequence of Ribosomal S1 Protein from Staphylococcus aureus

International Journal of Molecular Sciences ◽

10.3390/ijms23010524 ◽

2022 ◽

Vol 23 (1) ◽

pp. 524

Author(s):

Sergey V. Kravchenko ◽

Pavel A. Domnin ◽

Sergei Y. Grishin ◽

Alexander V. Panfilov ◽

Viacheslav N. Azev ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Peptides ◽

Pathogenic Bacteria ◽

Antimicrobial Effect ◽

Cell Penetrating Peptide ◽

Gram Negative Bacteria ◽

Antimicrobial Effects ◽

Bacterial Proteins ◽

Hybrid Peptides ◽

Successful Approach

The need to develop new antimicrobial peptides is due to the high resistance of pathogenic bacteria to traditional antibiotics now and in the future. The creation of synthetic peptide constructs is a common and successful approach to the development of new antimicrobial peptides. In this work, we use a simple, flexible, and scalable technique to create hybrid antimicrobial peptides containing amyloidogenic regions of the ribosomal S1 protein from Staphylococcus aureus. While the cell-penetrating peptide allows the peptide to enter the bacterial cell, the amyloidogenic site provides an antimicrobial effect by coaggregating with functional bacterial proteins. We have demonstrated the antimicrobial effects of the R23F, R23DI, and R23EI hybrid peptides against Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Pseudomonas aeruginosa, Escherichia coli, and Bacillus cereus. R23F, R23DI, and R23EI can be used as antimicrobial peptides against Gram-positive and Gram-negative bacteria resistant to traditional antibiotics.

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