Thermo-physicochemical investigation of molecular interactions in binary combination (dimethyl carbonate + methyl benzoate)

2017 ◽  
Vol 132 (1) ◽  
pp. 693-707 ◽  
Author(s):  
G. Jyothirmai ◽  
Sk. Md. Nayeem ◽  
Imran Khan ◽  
Ch. Anjaneyulu
2017 ◽  
Vol 07 (04) ◽  
Author(s):  
SK Beebi ◽  
SKM Nayeem ◽  
GR Satyanarayana ◽  
A Venkateshwara Rao ◽  
D Bala ◽  
...  

2008 ◽  
Vol 89 (8) ◽  
pp. 803-807 ◽  
Author(s):  
Dudu Wu ◽  
Wenming Chang ◽  
Xia Wen ◽  
Fukui Xiao ◽  
Junping Li ◽  
...  

Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.


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