The effects of folic acid and nitric oxide synthase inhibition on coronary flow and oxidative stress markers in isolated rat heart

2006 ◽  
Vol 300 (1-2) ◽  
pp. 177-183 ◽  
Author(s):  
Dragan Djurić ◽  
Ana Vušanović ◽  
Vladimir Jakovljević
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Folic Acid ◽  
Coronary Flow ◽  
Isolated Rat Heart ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Oxide Synthase ◽  
And Oxidative Stress ◽  
Isolated Rat

scholarly journals Effects of DL-Homocysteine Thiolactone on Cardiac Contractility, Coronary Flow, and Oxidative Stress Markers in the Isolated Rat Heart: The Role of Different Gasotransmitters

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Vladimir Zivkovic ◽  
Vladimir Jakovljevic ◽  
Olga Pechanova ◽  
Ivan Srejovic ◽  
Jovana Joksimovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Coronary Flow ◽  
Cardiac Contractility ◽  
Isolated Rat Heart ◽  
Oxidative Stress Markers ◽  
Homocysteine Thiolactone ◽  
Stress Markers ◽  
And Oxidative Stress ◽  
Isolated Rat

Considering the adverse effects of DL-homocysteine thiolactone hydrochloride (DL-Hcy TLHC) on vascular function and the possible role of oxidative stress in these mechanisms, the aim of this study was to assess the influence of DL-Hcy TLHC alone and in combination with specific inhibitors of important gasotransmitters, such as L-NAME, DL-PAG, and PPR IX, on cardiac contractility, coronary flow, and oxidative stress markers in an isolated rat heart. The hearts were retrogradely perfused according to the Langendorff technique at a 70 cm H2O and administered 10 μM DL-Hcy TLHC alone or in combination with 30 μM L-NAME, 10 μM DL-PAG, or 10 μM PPR IX. The following parameters were measured:dp/dtmax,dp/dtmin, SLVP, DLVP, MBP, HR, and CF. Oxidative stress markers were measured spectrophotometrically in coronary effluent through TBARS, NO2,O2-, and H2O2concentrations. The administration of DL-Hcy TLHC alone decreaseddp/dtmax, SLVP, and CF but did not change any oxidative stress parameters. DL-Hcy TLHC with L-NAME decreased CF,O2-, H2O2, and TBARS. The administration of DL-Hcy TLHC with DL-PAG significantly increaseddp/dtmax but decreased DLVP, CF, and TBARS. Administration of DL-Hcy TLHC with PPR IX caused a decrease indp/dtmax, SLVP, HR, CF, and TBARS.

scholarly journals The Effects Of L-Arginine And L-Name On Coronary Flow And Oxidative Stress In Isolated Rat Hearts

2015 ◽  
Vol 16 (4) ◽  
pp. 297-304
Author(s):  
Tanja Sobot ◽  
Amela Matavulj ◽  
Vladimir Jakovljevic ◽  
Tamara Nikolic ◽  
Vladimir Zivkovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Experimental Study ◽  
Superoxide Anion ◽  
Coronary Flow ◽  
Anion Radical ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Rat Hearts ◽  
And Oxidative Stress ◽  
Isolated Rat

AbstractThe aim of this experimental study was to assess the effects of the acute administration of L-arginine alone and in combination with L-NAME (a non-selective NO synthase inhibitor) on the coronary flow and oxidative stress markers in isolated rat hearts. The experimental study was performed on hearts isolated from Wistar albino rats (n=12, male, 8 weeks old, body mass of 180-200 g). Retrograde perfusion of the isolated preparations was performed using a modified method according to the Langendorff technique with a gradual increase in the perfusion pressure (40–120 cmH2O). The following values were measured in the collected coronary effluents: coronary flow, released nitrites (NO production marker), superoxide anion radical and the index of lipid peroxidation (measured as thiobarbiturate reactive substances). The experimental protocol was performed under controlled conditions, followed by the administration of L-arginine alone (1 mmol) and L-arginine (1 mmol) + L-NAME (30 μmol). The results indicated that L-arginine did not significantly increase the coronary flow or the release of NO, TBARS and the superoxide anion radical. These effects were partially blocked by the joint administration of L-arginine + L-NAME, which indicated their competitive effect. Hence, the results of our study do not demonstrate significant effects of L-arginine administration on the coronary flow and oxidative stress markers in isolated rat hearts.

scholarly journals The Effects of Diclofenac and Ibuprofen on Heart Function and Oxidative Stress Markers in the Isolated Rat Heart

2014 ◽  
Vol 15 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Maja Jevdjevic ◽  
Ivan Srejovic ◽  
Vladimir Zivkovic ◽  
Nevena Barudzic ◽  
Anica Petkovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Coronary Flow ◽  
Heart Function ◽  
Left Ventricular Pressure ◽  
Left Ventricular ◽  
Cox Inhibitors ◽  
Stress Markers ◽  
Pressure Development ◽  
And Oxidative Stress ◽  
Isolated Rat

ABSTRACT Eicosanoids lead to the promotion of inflammation, cause fever and pain and have many other eff ects. NSAIDs block the action of cyclooxygenase (COX) during the process of converting arachidonic acid into inflammatory mediators, thus reducing the symptoms of inflammation. Investigations focusing on nonselective COX inhibitors, used in high doses, revealed harmful eff ects on myocardial function. Th e aim of our study was to assess the eff ects of two nonselective NSAIDs, diclofenac and ibuprofen, on cardiodynamic parameters, coronary flow and oxidative stress biomarkers in isolated rat hearts. Th e hearts of male Wistar albino rats were excised and retrogradely perfused according to the Langendorff technique at gradually increased coronary perfusion pressures (40-120 cm H2O). Th e experiments were performed under controlled conditions (Krebs-Henseleit physiological solution). Th e hearts were perfused with 10 μmol/l diclofenac and 10 μmol/l ibuprofen. Th e heart function parameters, including the maximum rate of pressure development (dp/dt max), minimum rate of pressure development (dp/dt min), systolic left ventricular pressure (SLVP), diastolic left ventricular pressure (DLVP), mean perfusion pressure (MBP) and heart rate (HR), were continuously registered. Coronary flow (CF) was measured flowmetrically. Oxidative stress markers, including the index of lipid peroxidation measured as TBARS, nitric oxide measured through nitrites (NO2 -), superoxide anion radical (O2 -), and hydrogen peroxide (H2O2) in the coronary venous effluent, were assessed spectrophotometrically. Our results showed that diclofenac aff ected cardiodynamic parameters more significantly than did ibuprofen. Furthermore, the present data indicate that both estimated COX inhibitors do not promote the production of reactive oxygen species.

scholarly journals Metabolic, inflammatory and oxidative stress markers in the nitric oxide variation of hemodialysis subjects

2018 ◽  
Author(s):  
Andreza De Paula Santos Epifânio ◽  
Karla Pereira Balbino ◽  
Monica De Paula Jorge ◽  
Sonia M.R Ribeiro ◽  
Ana Vládia Bandeira Moreira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
And Oxidative Stress

scholarly journals Erratum to: The effects of cyclooxygenase and nitric oxide synthase inhibition on oxidative stress in isolated rat heart

2013 ◽  
Vol 381 (1-2) ◽  
pp. 313-313
Author(s):  
Nevena Barudzic ◽  
Drenka Turjacanin-Pantelic ◽  
Vladimir Zivkovic ◽  
Dragica Selakovic ◽  
Ivan Srejovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Oxide Synthase ◽  
Isolated Rat
Sign in / Sign up

Export Citation Format

Share Document