scholarly journals Transcriptome analysis of the irregular shape of shoot apical meristem in dt (dou tou) mutant of Brassica napus L.

2019 ◽  
Vol 39 (3) ◽  
Author(s):  
Ke-Ming Zhu ◽  
Shuo Xu ◽  
Kai-Xia Li ◽  
Sheng Chen ◽  
Sundus Zafar ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Transcriptome Analysis ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Irregular Shape ◽  
Brassica Napus L

scholarly journals Correction to: Transcriptome analysis of the irregular shape of shoot apical meristem in dt (duo tou) mutant of Brassica napus L.

2019 ◽  
Vol 39 (5) ◽  
Author(s):  
Ke-Ming Zhu ◽  
Shuo Xu ◽  
Kai-Xia Li ◽  
Sheng Chen ◽  
Sundus Zafar ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Transcriptome Analysis ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Irregular Shape ◽  
Brassica Napus L

Physiological influences in the development and function of the shoot apical meristem of microspore-derived embryos of Brassica napus ‘Topas’

2006 ◽  
Vol 84 (3) ◽  
pp. 371-383 ◽  
Author(s):  
Nicole S. Ramesar-Fortner ◽  
Edward C. Yeung
Keyword(s):  
Brassica Napus ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Radial Symmetry ◽  
Brassica Napus L ◽  
Conversion Frequency ◽  
Dramatic Decline ◽  
Meristem Development ◽  
Methylpropionic Acid ◽  
And Function

The effect of auxins and abscisic acid (ABA) on shoot apical meristem development and function was analyzed in microspore-derived embryos of Brassica napus L. ‘Topas’. Embryos were treated with an auxin transport inhibitor, tri-iodobenzoic acid (TIBA) or exogenous indole-3-acetic acid (IAA) at various stages during their development. If embryos were treated at the preglobular or globular stages of embryogenesis, they developed one fused cotyledon indicating a continuation of radial symmetry. However, if treated later in development, embryos were comparable to the control and formed two separate cotyledons. To demonstrate that this was not a pharmacological effect, an auxin antagonist, 2-(p-chlorophenoxy)-2-methylpropionic acid, included with the TIBA treatment produced normal embryos with two separate cotyledons. Histological observations revealed that the shoot apical meristem of the embryos treated with TIBA or IAA at the preglobular and globular stages was altered. Preglobular and globular stage embryos that were treated with TIBA exhibited a dramatic decline in conversion frequency compared with their controls. Embryos that were treated later in development had conversion frequencies comparable to their controls, and their shoot apical meristems also were similar to controls. Application of ABA at the preglobular and globular stages maintained meristem integrity and improved embryo conversion. However, ABA could not reverse the TIBA effect.

Tri-iodobenzoic acid affects shoot apical meristem formation and function in zygotic embryos of Brassica napus cv. Topas

2001 ◽  
Vol 79 (3) ◽  
pp. 265-273
Author(s):  
Nicole S Ramesar-Fortner ◽  
Edward C Yeung
Keyword(s):  
Brassica Napus ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Bilateral Symmetry ◽  
Shoot Meristem ◽  
Zygotic Embryos ◽  
Brassica Napus L ◽  
Dramatic Decline ◽  
And Function ◽  
Iodobenzoic Acid

The effect of an auxin transport inhibitor, tri-iodobenzoic acid, on the formation and subsequent function of the shoot apical meristem in zygotic embryos of Brassica napus L. was examined. Globular and heart stage embryos were cultured in the presence of tri-iodobenzoic acid. Only embryos at the globular stage of embryogenesis were affected by tri-iodobenzoic acid. Upon treatment, the embryos did not develop two separate cotyledons and, therefore, did not attain bilateral symmetry. Furthermore, the shoot apical meristem of these embryos was altered, as a characteristic tunica-corpus organization was not observed. The cells of the surface layer continued to maintain their meristematic characteristics and divided mainly in the anticlinal direction. The subapical cells differentiated into parenchyma cells and continued to expand such that a corpus organization never developed. Therefore, by the end of the culture period, the shoot apical meristem appeared very broad and shallow. Treated embryos that were transferred to a conversion medium exhibited a dramatic decline and delay in the formation of leaves compared with untreated embryos. Histological observations revealed that the shoot apical meristem continued to expand in width. A low percentage of treated embryos were able to convert; however, leaves were produced from a new shoot meristem that developed in the axillary position. No morphological or histological changes were evident when the embryos were treated later, at the heart stage of embryogenesis.Key words: auxin, Brassica napus, embryo, shoot apical meristem, tri-iodobenzoic acid.

Comparative genome and transcriptome analysis unravels key factors of nitrogen use efficiency in Brassica napus L

2019 ◽  
Vol 43 (3) ◽  
pp. 712-731 ◽  
Author(s):  
Quan Li ◽  
Guangda Ding ◽  
Ningmei Yang ◽  
Philip John White ◽  
Xiangsheng Ye ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Nitrogen Use Efficiency ◽  
Transcriptome Analysis ◽  
Key Factors ◽  
Brassica Napus L ◽  
Nitrogen Use ◽  
Comparative Genome ◽  
Use Efficiency

scholarly journals Fine-mapping and transcriptome analysis of a candidate gene controlling plant height in Brassica napus L.

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Xiaodong Wang ◽  
Ming Zheng ◽  
Hongfang Liu ◽  
Liang Zhang ◽  
Feng Chen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Candidate Gene ◽  
Plant Height ◽  
Fine Mapping ◽  
Transcriptome Analysis ◽  
Brassica Napus L

scholarly journals Characterization of the BnA10.tfl1 Gene Controls Determinate Inflorescence Trait in Brassica napus L.

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 722 ◽  
Author(s):  
Yongpeng Jia ◽  
Kaixiang Li ◽  
Haidong Liu ◽  
Lingxiong Zan ◽  
Dezhi Du
Keyword(s):  
Brassica Napus ◽  
Shoot Apex ◽  
Apical Meristem ◽  
Brassica Napus L ◽  
Reading Frame ◽  
Terminal Flower ◽  
Terminal Flower 1 ◽  
Oilseed Breeding ◽  
Phosphatidylethanolamine Binding Protein

Determinate inflorescences have a significant effect on the genetic improvement of rapeseed, so understanding the molecular function underlying the inflorescence trait may be beneficial to oilseed breeding. A previous study found candidate gene BnTFL1 (Terminal Flower 1) for control of the inflorescence trait on Brassica napus chromosome A10 (16,627–16,847 kb). However, little is known about the function of the BnTFL1 gene in B. napus. In this study, we firstly studied the formation of the shoot apical meristem and gene expression in indeterminate and determinate inflorescences; the results showed that the inflorescence architecture and BnA10.TFL1 expression showed significant differences in the shoot apex at the budding stage. Then, two alleles (named BnA10.TFL1 gene from indeterminate and BnA10.tfl1 gene from determinate) were cloned and sequence-analyzed; the results suggest that the open reading frame of the alleles comprises 537 bp, encodes 178 amino acids containing a conserved phosphatidylethanolamine-binding protein (PEBP) domain, and shares high similarity with Arabidopsis thaliana TFL1. To analyze the function of BnA10.TFL1, the BnA10.TFL1 gene was introduced into the determinate A. thaliana tfl1 mutant and B. napus 571 line by complementation experiment. The determinate traits were restored to indeterminate, and expression of BnA10.TFL1 was increased in the indeterminate shoot apex. These results reveal that BnA10.tfl1 is a gene controlling the determinate inflorescence trait. Moreover, the BnA10.TFL1 protein was localized to the nucleus, cytoplasm, and plasma membrane. Collectively, the results of this study help us to understand the molecular mechanism of determinate inflorescences and will provide a reliable research basis for the application of determinate inflorescences in B. napus.

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