Forced expression of Mdmyb10, a myb transcription factor gene from apple, enhances tolerance to osmotic stress in transgenic Arabidopsis

2010 ◽  
Vol 38 (1) ◽  
pp. 205-211 ◽  
Author(s):  
Jian-Jie Gao ◽  
Zhen Zhang ◽  
Ri-He Peng ◽  
Ai-Sheng Xiong ◽  
Jing Xu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Osmotic Stress ◽  
Transgenic Arabidopsis ◽  
Myb Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene

Overexpression of a wheat MYB transcription factor gene, TaMYB56-B, enhances tolerances to freezing and salt stresses in transgenic Arabidopsis

Gene ◽  
2012 ◽  
Vol 505 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Lichao Zhang ◽  
Guangyao Zhao ◽  
Chuan Xia ◽  
Jizeng Jia ◽  
Xu Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transgenic Arabidopsis ◽  
Myb Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene

Soybean Transcription Factor Gene GmNF-YCa Enhances Osmotic Stress Tol-erance of Transgenic Arabidopsis

2017 ◽  
Vol 43 (8) ◽  
pp. 1161
Author(s):  
Min LI ◽  
Tai-Fei YU ◽  
Zhao-Shi XU ◽  
Shuang-Xi ZHANG ◽  
Dong-Hong MIN ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Osmotic Stress ◽  
Transgenic Arabidopsis ◽  
Transcription Factor Gene ◽  
Factor Gene

scholarly journals Expression of MYB transcription factor gene ZmMYB59 affects seed germination in Nicotiana tabacum and Oryza sativa

2020 ◽  
Author(s):  
Kaihui Zhai ◽  
Guangwu Zhao ◽  
Hongye Jiang ◽  
Caixia Sun ◽  
Jingyu Ren
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Seed Germination ◽  
Transgenic Tobacco ◽  
Germination Rate ◽  
Myb Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Myb Transcription Factors ◽  
Vigor Index

Abstract Background MYB transcription factors are involved in many biological processes, including metabolism, development and responses to biotic and abiotic stresses. In our previous work, a new MYB transcription factor gene, ZmMYB59 was induced by deep sowing and down-regulated during maize seed germination via Real-Time PCR. However, there are few reports on seed germination regulated by MYB proteins and the functions of ZmMYB59 remain unknown. Results In this study, to examine its functions, Agrobacterium -mediated transformation was exploited to generate ZmMYB59 transgenic tobacco and rice. In T 2 generation transgenic tobacco, germination rate, germination index, vigor index and hypocotyl length were significantly decreased by 25.0~50.9%, 34.5~54.4%, 57.5~88.3% and 21.9~31.2% compared to wild-type (WT) lines. In T 2 generation transgenic rice, germination rate, germination index, vigor index and mesocotyl length were notably reduced by 39.1~53.8%, 51.4~71.4%, 52.5~74.0% and 28.3~41.5%, respectively. On this basis, relative physiological indicators were determined. The activities of catalase, peroxidase, superoxide dismutase, ascorbate peroxidase and proline content of transgenic lines were significantly lower than those of WT, suggesting that ZmMYB59 reduced their antioxidant capacity. As well, ZmMYB59 expression extremely inhibited the synthesis of gibberellin A1 (GA 1 ) and cytokinin (CTK), and promoted the synthesis of abscisic acid (ABA) concurrently, which implied that seed germination was repressed by ZmMYB59 in hormone levels. Furthermore, cell length and cell number of hypocotyl/mesocotyl in transgenic plants were notably decreased. Conclusions Taken together, it proposed that ZmMYB59 plays a negative regulation during seed germination in tobacco and rice, which also contributes to illuminate the molecular mechanisms regulated by MYB transcription factors.

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