Prenatal Hypoxia Leads to Impaired Formation of Nervous Tissue in the Entorhinal Area of the Cerebral Cortex in Rats

Replicas of fracture faces along a variety of frozen membranes have revealed a particulate component, with replicas of individual particles measuring 100 to 200 Å in diameter. The relation of these small particles to membrane ultrastructure is questionable and there is even some controversy as to whether the particles represent true membrane components, material adsorbed onto membranes from adjacent compartments, or a contaminent adsorbed onto the frozen fracture face prior to replication with carbon and platinum. In this study, replicas of fracture faces of frozen nervous tissue were surveyed in an effort to find if consistent patterns of particle distribution could be demonstrated on nervous system membranes.Rat retinas, cerebral cortex and corpus callosum were protected with glycerol or dimethylsulfoxide and freeze-fractured with a razor blade in a three tier apparatus as described in detail by Bullivant and Ames.1 Following replication in an evaporator (Kinney PW 400), the tissue was dissolved and fragments of replica were washed, picked up on grids and examined in the electron microscope. No etching, as it is defined by Moor, was involved in producing these fracture faces for replication.

Download Full-text

The Fixation of Central Nervous Tissue and the Analysis of Electron Micrographs of the Neuropil, with Special Reference to the Cerebral Cortex

Contemporary Research Methods in Neuroanatomy ◽

10.1007/978-3-642-85986-1_4 ◽

1970 ◽

pp. 56-76 ◽

Cited By ~ 88

Author(s):

Alan Peters

Keyword(s):

Cerebral Cortex ◽

Special Reference ◽

Electron Micrographs ◽

Nervous Tissue ◽

Central Nervous Tissue

Download Full-text

Projections of the entorhinal area to the striatum, nucleus accumbens, and cerebral cortex in the guinea pig

The Journal of Comparative Neurology ◽

10.1002/cne.902380306 ◽

1985 ◽

Vol 238 (3) ◽

pp. 308-322 ◽

Cited By ~ 53

Author(s):

K. E. Sørensen

Keyword(s):

Cerebral Cortex ◽

Nucleus Accumbens ◽

Guinea Pig ◽

Entorhinal Area

Download Full-text

SELECTIVE STAINING OF NERVOUS TISSUE FOR LIGHT MICROSCOPY FOLLOWING PREPARATION FOR ELECTRON MICROSCOPY

Journal of Histochemistry & Cytochemistry ◽

10.1177/16.12.808 ◽

1968 ◽

Vol 16 (12) ◽

pp. 808-814 ◽

Cited By ~ 54

Author(s):

LILLIAN R. BERKOWITZ ◽

OLGA FIORELLO ◽

LAWRENCE KRUGER ◽

DAVID S. MAXWELL

Keyword(s):

Electron Microscopy ◽

Cerebral Cortex ◽

Silver Nitrate ◽

Nervous Tissue ◽

Cell Structure ◽

Differential Staining ◽

Selective Staining ◽

Axon Terminals ◽

Ammoniacal Silver ◽

Good Contrast

The suitability and selectivity of several dyes have been tested on plastic-embedded sections of nervous tissue (0.5-1 µ) prepared for electron microscopy. Methods for plastic extraction and selective staining are described which provide satisfactory differentiation of principle cytologic features with good contrast and clarity of detail. The introduction of a hematoxylin "lake" has achieved the differential staining of oligodendroglia and astrocytes in cerebral cortex. The argyrophilia of cells and cell structure following 10-20% silver nitrate or silver nitrate in combination with ammoniacal silver permits identification of axon terminals.

Download Full-text