Rapid evaluation of the functional activity of the hepatic monooxygenase system

2007 ◽  
Vol 41 (12) ◽  
pp. 631-633
Author(s):  
V. G. Gorokhova ◽  
A. G. Gorokhov ◽  
E. E. Kuznetsova ◽  
A. A. Runovich
Keyword(s):  
Functional Activity ◽  
Rapid Evaluation ◽  
Monooxygenase System ◽  
Hepatic Monooxygenase

scholarly journals Evaluating live microbiota biobanking using an ex vivo microbiome assay and metaproteomics

2021 ◽  
Author(s):  
Xu Zhang ◽  
Krystal Walker ◽  
Janice Mayne ◽  
Leyuan Li ◽  
Zhibin Ning ◽  
...  
Keyword(s):  
Fecal Sample ◽  
Functional Activity ◽  
Fecal Microbiota Transplantation ◽  
Ex Vivo ◽  
Fecal Microbiota ◽  
Rapid Evaluation ◽  
Sample Collection ◽  
Functional Responses ◽  
Clinical Investigations

Biobanking of live microbiota is becoming indispensable for mechanistic and clinical investigations of drug-microbiome interactions and fecal microbiota transplantation. However, there is a lack of methods to rapidly and systematically evaluate whether the biobanked microbiota maintains their cultivability and functional activity. In this study, we use a rapid ex vivo microbiome assay and metaproteomics to evaluate the cultivability and the functional responses of biobanked microbiota to treatment with a prebiotic (fructo-oligosaccharide, FOS). Our results indicate that the microbiota cultivability and their functional responses to FOS treatment were well maintained by freezing in a deoxygenated glycerol buffer at -80°C for 12 months. We also demonstrate that the fecal microbiota is functionally stable for 48 hours on ice in a deoxygenated glycerol buffer, allowing off-site fecal sample collection and shipping to laboratory for live microbiota biobanking. This study provides a method for rapid evaluation of the cultivability of biobanked live microbiota. Our results show minimal detrimental influences of long-term freezing in deoxygenated glycerol buffer on the cultivability of fecal microbiota.

Effects of Environmental pH on the Hepatic Mixed Function Oxidases in Atlantic Salmon (Salmo salar)

1991 ◽  
Vol 48 (3) ◽  
pp. 445-447 ◽  
Author(s):  
Douglas E. Willis ◽  
Allison J. Edwards ◽  
Richard F. Addison
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Cytochromes P450 ◽  
Microsomal Protein ◽  
The Other ◽  
Monooxygenase System ◽  
Atlantic Salmon Salmo Salar ◽  
Body Weights ◽  
Hepatic Monooxygenase ◽  
Pyrene Hydroxylase

Atlantic salmon (Salmo salar) were held for some months in the Medway River, N.S., at pH 5.2–5.5 and in the Westfield River, N.S., in either untreated ("acidic") water at pH 4.7–5.2 or limed water at pH 51.–5.9 and were examined for effects on hepatic monooxygenases. Fish from the Westfield'acidic regime had lower body weights than those from the other two regimes, but similar liver weights. Females generally had higher hepatic microsomal protein contents than males, but this variable was not affected by environmental pH. Westfield acidic fish usually had higher benzo[a]pyrene hydroxylase and lower ethoxyresorufin O-deethylase activity than those from the other two regimes. Cytochromes P450 and b5 showed no clear difference between groups. Although the different pH environments affected reproductive success and steroid hormone metabolism, such changes were not reflected in these components of the hepatic monooxygenase system.

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