Validation of an Analytical HPLC Method for a New Diagnostic Octreotide Derivative for Neuroendocrine Tumors

Sporotrichosis occurs worldwide and is caused by the fungus Sporothrix brasiliensis. This agent has a high zoonotic potential and is transmitted mainly by bites and scratches from infected felines. A new association between the drugs clotrimazole and itraconazole is shown to be effective against S. brasiliensis yeasts. This association was formulated as a microemulsion containing benzyl alcohol as oil, Tween® 60 and propylene glycol as surfactant and cosurfactant, respectively, and water. Initially, the compatibility between clotrimazole and itraconazole was studied using differential scanning calorimetry (DSC), thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), and X-ray powder diffraction (PXRD). Additionally, a simple and efficient analytical HPLC method was developed to simultaneously determine the concentration of clotrimazole and itraconazole in the novel microemulsion. The developed method proved to be efficient, robust, and reproducible for both components of the microemulsion. We also performed an accelerated stability study of this formulation, and the developed analytical method was applied to monitor the content of active ingredients. Interestingly, these investigations led to the detection of a known clotrimazole degradation product whose structure was confirmed using NMR and HRMS, as well as a possible interaction between itraconazole and benzyl alcohol.

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Pharmacokinetics of fluoroquinolones in critical care patients: A bio-analytical HPLC method for the simultaneous quantification of ofloxacin, ciprofloxacin and moxifloxacin in human plasma

Journal of Chromatography B ◽

10.1016/j.jchromb.2009.02.039 ◽

2009 ◽

Vol 877 (10) ◽

pp. 961-967 ◽

Cited By ~ 47

Author(s):

Julie De Smet ◽

Koen Boussery ◽

Kirsten Colpaert ◽

Peter De Sutter ◽

Peter De Paepe ◽

...

Keyword(s):

Critical Care ◽

Human Plasma ◽

Hplc Method ◽

Simultaneous Quantification ◽

Analytical Hplc ◽

Critical Care Patients

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Development and Validation of a Simple and Selective Analytical HPLC Method for the Quantification of Oxaliplatin

Journal of Chemistry ◽

10.1155/2015/812701 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 6

Author(s):

Breno Noronha Matos ◽

Paula Martins de Oliveira ◽

Thaiene Avila Reis ◽

Taís Gratieri ◽

Marcílio Cunha-Filho ◽

...

Keyword(s):

Future Development ◽

Limit Of Detection ◽

Hplc Method ◽

Limit Of Quantification ◽

Pharmaceutical Drug ◽

Skin Cancers ◽

Isocratic Hplc ◽

Precision And Accuracy ◽

Development And Validation ◽

Analytical Hplc

Considering that currently published oxaliplatin (OXPt) analytical methods require complex procedures and equipment, the objective of this paper was to present the validation of a simple, rapid, precise, and specific isocratic HPLC method for quantification of OXPt. Such method will be essential during the future development of innovative, topical drug formulations for the treatment of mucosal and skin cancers. Validated method demonstrated OXPt separation without interference from the solvents or polymers with the most relevance for developing of bioadhesive pharmaceutical drug carries (chitosan and poloxamer). Method was linear(r>0.999)over studied OXPt concentration range (0.5–15.0 µg/mL) with acceptable precision and accuracy. Limit of detection (LOD) and limit of quantification (LOQ) were 0.099 µg/mL and 0.331 µg/mL, respectively.

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