The Effect of Surface Charges on the Cellular Uptake of Liposomes Investigated by Live Cell Imaging

2017 ◽  
Vol 34 (4) ◽  
pp. 704-717 ◽  
Author(s):  
Ji Hee Kang ◽  
Woo Young Jang ◽  
Young Tag Ko
Keyword(s):  
Cellular Uptake ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Surface Charges ◽  
Effect Of Surface

Cucurbit[7]uril-conjugated dyes as live cell imaging probes: investigation on their cellular uptake and excretion pathways

2019 ◽  
Vol 17 (25) ◽  
pp. 6215-6220 ◽  
Author(s):  
Meng Li ◽  
Ara Lee ◽  
Sungwan Kim ◽  
Annadka Shrinidhi ◽  
Kyeng Min Park ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Intracellular Uptake ◽  
Imaging Probes

The potential of cucurbit[7]uril derivatives as live cell imaging probes is demonstrated by investigating the intracellular uptake and excretion of dye-conjugated cucurbit[7]uril derivatives.

scholarly journals Luminescent bis(benzo[d]thiazolyl)quinoxaline: facile synthesis, nucleic acid and protein BSA interaction, live-cell imaging, biopharmaceutical research and cancer theranostic application

RSC Advances ◽  
2019 ◽  
Vol 9 (16) ◽  
pp. 8748-8752 ◽  
Author(s):  
Lavanya Thilak Babu ◽  
Gajanan Raosaheb Jadhav ◽  
Priyankar Paira
Keyword(s):  
Nucleic Acid ◽  
Cellular Uptake ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Fluorescence Properties ◽  
Facile Synthesis ◽  
Bsa Interaction

A series of luminescent bis(benzo[d]thiazolyl)quinoxalines have been synthesized and their fluorescence properties, anticancer potency, DNA and BSA interactions, cellular uptake, and metabolic stabilities are investigated.

scholarly journals Development and In Vitro Characterization of Transferrin-Decorated Nanoemulsion Utilizing Hydrophobic Ion Pairing for Targeted Cellular Uptake

2021 ◽  
Author(s):  
Sergey Zaichik ◽  
Christian Steinbring ◽  
Julian David Friedl ◽  
Andreas Bernkop-Schnürch
Keyword(s):  
Drug Delivery ◽  
Flow Cytometry ◽  
Zeta Potential ◽  
Droplet Size ◽  
Cellular Uptake ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Ion Pairing ◽  
Live Cell ◽  
Hydrophobic Ion Pairing

Abstract Purpose The aim of this study was to develop transferrin-conjugated nanoemulsions utilizing hydrophobic ion pairing for a targeted cellular uptake. Methods In the lipophilic phase of nanoemulsion composed of 60% oleic acid, 30% Capmul MCM EP and 10% Span 85, 1% cetyltrimethylammonium bromide (CTAB) and 3% phosphatidic acid (PA) were incorporated. After emulsification, the resulting droplets were decorated with human protein transferrin via hydrophobic ion pairing with PA and characterized regarding droplet size and zeta potential. Subsequently, cellular uptake of transferrin-conjugated nanoemulsion was investigated on Caco-2 and HeLa cell lines and determined by flow cytometry, cell lysis method and live cell imaging using confocal laser scanning microscopy. Results The nanoemulsion showed a droplet size of 123.03 ± 2.1 nm and zeta potential of − 54.5 mV that changed because of the surface decoration with transferrin to 182.7 ± 0.2 and + 30.2 mV, respectively. Within the uptake studies utilizing flow cytometry, transferrin-conjugated nanoemulsion showed a 5.2-fold higher uptake in Caco-2 cells and twofold improvement in case of HeLa cells compared with unmodified formulation. The outcome was confirmed visually via live cell imaging. Conclusion According to the results, transferrin-conjugated nanoemulsion might be considered as a promising drug delivery system for a selective receptor-mediated drug delivery.

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