Role of a novel pathogen-induced pepper C3–H–C4 type RING-finger protein gene, CaRFP1, in disease susceptibility and osmotic stress tolerance

2006 ◽  
Vol 63 (4) ◽  
pp. 571-588 ◽  
Author(s):  
Jeum Kyu Hong ◽  
Hyong Woo Choi ◽  
In Sun Hwang ◽  
Byung Kook Hwang
Keyword(s):  
Osmotic Stress ◽  
Stress Tolerance ◽  
Disease Susceptibility ◽  
Protein Gene ◽  
Ring Finger ◽  
Ring Finger Protein ◽  
Osmotic Stress Tolerance ◽  
Finger Protein

scholarly journals Arkadia (RING Finger Protein 111) Mediates Sumoylation-Dependent Stabilization of Nrf2 Through K48-Linked Ubiquitination

2018 ◽  
Vol 46 (1) ◽  
pp. 418-430 ◽  
Author(s):  
Deneshia J. McIntosh ◽  
Treniqka S. Walters ◽  
Ifeanyi J. Arinze ◽  
Jamaine Davis
Keyword(s):  
Gene Transcription ◽  
Ring Finger ◽  
Nuclear Body ◽  
Promyelocytic Leukemia ◽  
Heme Oxygenase 1 ◽  
Ring Finger Protein ◽  
Whole Cell ◽  
Cell Lysates ◽  
Finger Protein

Background/Aims: The transcription factor Nrf2 is a master regulator of the antioxidant defense system, protecting cells from oxidative damage. We previously reported that the SUMO-targeted E3 ubiquitin ligase (STUbL), RING finger protein 4 (RNF4) accelerated the degradation rate of Nrf2 in promyelocytic leukemia-nuclear body (PML-NB)-enriched fractions and decreased Nrf2-mediated gene transcription. The mechanisms that regulate Nrf2 nuclear levels are poorly understood. In this study, we aim to explore the role of the second mammalian STUbL, Arkadia/RNF111 on Nrf2. Methods: Arkadia mediated ubiquitination was detected using co-immunoprecipitation assays in which whole cell lysates were immunoprecipated with anti-Nrf2 antibody and Western blotted with anti-hemagglutinin (HA) antibody or anti-Lys-48 ubiquitin-specific antibody. The half-life of Nrf2 was detected in whole cell lysates and promyelocytic leukemia-nuclear body enriched fractions by cycloheximide-chase. Reporter gene assays were performed using the antioxidant response element (ARE)-containing promoter Heme oxygenase-1 (HO-1). Results: We show that Arkadia/RNF111 is able to ubiquitinate Nrf2 resulting in the stabilization of Nrf2. This stabilization was mediated through Lys-48 ubiquitin chains, contrary to traditionally degradative role of Lys-48 ubiquitination, suggesting that Lys-48 ubiquitination of Nrf2 protects Nrf2 from degradation thereby allowing Nrf2-dependent gene transcription. Conclusion: Collectively, these findings highlight a novel mechanism to positively regulate nuclear Nrf2 levels in response to oxidative stress through Arkadia-mediated K48-linked ubiquitination of Nrf2.

Isolation of a cDNA for a novel human RING finger protein gene, RNF18, by the virtual transcribed sequence (VTS) approach

2000 ◽  
Vol 1493 (3) ◽  
pp. 349-355 ◽  
Author(s):  
Tsutomu Yoshikawa ◽  
Naohiko Seki ◽  
Takanori Azuma ◽  
Yasuhiko Masuho ◽  
Masa-aki Muramatsu ◽  
...  
Keyword(s):  
Protein Gene ◽  
Ring Finger ◽  
Ring Finger Protein ◽  
Finger Protein

scholarly journals Structural basis for role of ring finger protein RNF168 RING domain

Cell Cycle ◽  
2013 ◽  
Vol 12 (2) ◽  
pp. 312-321 ◽  
Author(s):  
Xiaoqin Zhang ◽  
Jie Chen ◽  
Minhao Wu ◽  
Huakai Wu ◽  
Aloysius Wilfred Arokiaraj ◽  
...  
Keyword(s):  
Ring Finger ◽  
Ring Domain ◽  
Structural Basis ◽  
Ring Finger Protein ◽  
Finger Protein

Physiological and metabolomic analysis of a knockout mutant suggests a critical role of MtP5CS3 gene in osmotic stress tolerance of Medicago truncatula

Euphytica ◽  
2013 ◽  
Vol 193 (1) ◽  
pp. 101-120 ◽  
Author(s):  
Minh Luan Nguyen ◽  
Goon-Bo Kim ◽  
Sun-Hee Hyun ◽  
Seok-Young Lee ◽  
Chae-Young Lee ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Stress Tolerance ◽  
Medicago Truncatula ◽  
Critical Role ◽  
Metabolomic Analysis ◽  
Knockout Mutant ◽  
Osmotic Stress Tolerance

scholarly journals Abstract 4825: Functional role of Ring Finger Protein 43 in intestinal stem cell during colorectal tumorigenesis

2017 ◽  
Author(s):  
Tsugio Eto ◽  
Takatsugu Ishimoto ◽  
Eri Oda ◽  
Daisuke Kuroda ◽  
Kota Arima ◽  
...  
Keyword(s):  
Stem Cell ◽  
Functional Role ◽  
Ring Finger ◽  
Intestinal Stem Cell ◽  
Ring Finger Protein ◽  
Colorectal Tumorigenesis ◽  
Finger Protein
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