Differential nitrogen-use efficiency in wheat parents of doubled-haploid mapping populations

Nitrogen (N) is one of the primary macronutrients required for crop growth and yield. This nutrient is especially limiting in the dry and low fertility soils where pearl millet [Pennisetum glaucum (L.) R. Br] is typically grown. Globally, pearl millet is the sixth most important cereal grown by subsistence farmers in the arid and semi-arid regions of sub-Saharan Africa and the Indian subcontinent. Most of these agro-ecologies have low N in the root zone soil strata. Therefore, there is an immense need to identify lines that use nitrogen efficiently. A set of 380 diverse pearl millet lines consisting of a global diversity panel (345), parents of mapping populations (20), and standard checks (15) were evaluated in an alpha-lattice design with two replications, 25 blocks, a three-row plot for 11 nitrogen use efficiency (NUE) related traits across three growing seasons (Summer 2017, Rainy 2017, and Summer 2018) in an N-depleted precision field under three different N levels (0%-N0, 50%-N50, 100%-N100 of recommended N, i.e., 100 kg ha–1). Analysis of variance revealed significant genetic variation for NUE-related traits across treatments and seasons. Nitrogen in limited condition (N0) resulted in a 27.6 and 17.6% reduction in grain yield (GY) and dry stover yield (DSY) compared to N50. Higher reduction in GY and DSY traits by 24.6 and 23.6% were observed under N0 compared to N100. Among the assessed traits, GY exhibited significant positive correlations with nitrogen utilization efficiency (NUtE) and nitrogen harvest index (NHI). This indicated the pivotal role of N remobilization to the grain in enhancing yield levels. Top 25 N-insensitive (NIS-top grain yielders) and N-sensitive (NS-poor grain yielders) genotypes were identified under low N conditions. Out of 25 NIS lines, nine genotypes (IP 10820, IP 17720, ICMB 01222-P1, IP 10379, ICMB 89111-P2, IP 8069, ICMB 90111-P2, ICMV IS89305, and ICMV 221) were common with the top 25 lines for N100 level showing the genotype plasticity toward varying N levels. Low N tolerant genotypes identified from the current investigation may help in the identification of genomic regions responsible for NUE and its deployment in pearl millet breeding programs through marker-assisted selection (MAS).

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Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency Than Parental Lines by F1 Microspore Embryogenesis

Plants ◽

10.3390/plants10081588 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1588

Author(s):

Hongwei Xu ◽

Yingbo Li ◽

Runhong Gao ◽

Rugen Xu ◽

Guimei Guo ◽

...

Keyword(s):

Nitrogen Use Efficiency ◽

Doubled Haploid ◽

Molecular Mechanisms ◽

Field Experiments ◽

Microspore Embryogenesis ◽

Doubled Haploid Line ◽

Mapk Signaling ◽

Nitrogen Use ◽

Use Efficiency ◽

Low Nitrogen

Creating varieties with high nitrogen use efficiency (NUE) is crucial for sustainable agriculture development. In this study, a superior barley doubled haploid line (named DH45) with improved NUE was produced via F1 microspore embryogenesis with three rounds of screening in different nitrogen levels by hydroponic and field experiments. The molecular mechanisms responsible for the NUE of DH45 surpassing that of its parents were investigated by RNA-seq analysis. A total of 1027 differentially expressed genes (DEGs) were identified that were up- or down-regulated in DH45 under low nitrogen conditions but showed no significant differences in the parents. GO analysis indicated that genes involved in nitrogen compound metabolic processes were significantly enriched in DH45 compared with the parents. KEGG analysis showed the MAPK signaling pathway plant to be highly enriched in DH45 relative to its parents, as well as genes involved in alanine, aspartate and glutamate metabolism, and arginine biosynthesis. In conclusion, our study revealed the potential to fix trait superiority in a line by combining crossing with F1 microspore culture technologies in future crop breeding and also identified several candidate genes that are expressed in shoots and may enable barley to cope with low-nitrogen stress.

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Faculty Opinions recommendation of Leaf-level nitrogen use efficiency: definition and importance.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717964300.793465842 ◽

2012 ◽

Author(s):

Christian Körner

Keyword(s):

Nitrogen Use Efficiency ◽

Nitrogen Use ◽

Leaf Level ◽

Use Efficiency

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Faculty Opinions recommendation of PvNAC1 and PvNAC2 Are Associated with Leaf Senescence and Nitrogen Use Efficiency in Switchgrass.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726131278.793514339 ◽

2016 ◽

Author(s):

Elizabeth Hood

Keyword(s):

Leaf Senescence ◽

Nitrogen Use Efficiency ◽

Nitrogen Use ◽

Use Efficiency

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ROLE OF CONSERVATION AGRICULTURE IN IMPROVING PRODUCTION WATER AND NITROGEN EFFICIENCY OF WHEAT UNDER RAINFED CONDITIONS

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v51i4.1093 ◽

2020 ◽

Vol 51 (4) ◽

pp. 1139-1148

Author(s):

Othman & et al.

Keyword(s):

Crop Rotation ◽

Nitrogen Use Efficiency ◽

Agricultural Research ◽

Research Work ◽

Conservation Agriculture ◽

Nitrogen Efficiency ◽

Research Station ◽

Wheat Varieties ◽

Nitrogen Use ◽

Use Efficiency

The research work was conducted in Izra’a Research station, which affiliated to the General Commission for Scientific Agricultural Research (GCSAR), during the growing seasons (2016 – 2017; 2017 – 2018), in order to evaluate the response of two durum wheat verities (Douma3 and Cham5) and two bread wheat varieties (Douma4 and Cham6) to Conservation Agriculture (CA) as a full package compared with Conventional Tillage system (CT) under rainfed condition using lentils (Variety Edleb3) in the applied crop rotation. The experiment was laid according to split-split RCBD with three replications. The average of biological yield, grain yield, rainwater use efficiency and nitrogen use efficiency was significantly higher during the first growing season, under conservation agriculture in the presence of crop rotation, in the variety Douma3 (7466 kg. ha-1, and 4162kg. ha-1, 19.006 kg ha-1 mm-1, 39.62 kg N m-2respectively). The two varieties Douma3 and Cham6 are considered more responsive to conservation agriculture system in the southern region of Syria, because they recorded the highest grain yields (2561, 2385 kg ha-1 respectively) compared with the other studied varieties (Cham5 and Douma4) (1951 and 1724 kg ha-1 respectively). They also exhibited the highest values of both rainwater and nitrogen use efficiency.

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