Agrobacterium-Mediated In Planta Transformation of Field Bean (Lablab purpureus L.) and Recovery of Stable Transgenic Plants Expressing the cry1AcF Gene

2011 ◽  
Vol 30 (1) ◽  
pp. 67-78 ◽  
Author(s):  
E. Keshamma ◽  
Rohini Sreevathsa ◽  
A. Manoj Kumar ◽  
Kalpana N. Reddy ◽  
M. Manjulatha ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Field Bean ◽  
In Planta Transformation ◽  
Lablab Purpureus ◽  
In Planta

A rapid, novel and high-throughput identification of putative bell pepper transformants generated through in planta transformation approach

2011 ◽  
Vol 129 (4) ◽  
pp. 898-903 ◽  
Author(s):  
Arthikala Manoj Kumar ◽  
Kalpana N. Reddy ◽  
M. Manjulatha ◽  
Elizabeth S. Arellano ◽  
Rohini Sreevathsa ◽  
...  
Keyword(s):  
High Throughput ◽  
Bell Pepper ◽  
In Planta Transformation ◽  
In Planta ◽  
Transformation Approach

scholarly journals Optimization of Agrobacterium-mediated transformation conditions of prospective genotypes of winter bread wheat by in planta method

2021 ◽  
Vol 28 ◽  
pp. 66-71
Author(s):  
O. V. Dubrovna ◽  
L. V. Slivka
Keyword(s):  
Transgenic Plants ◽  
Bread Wheat ◽  
Optical Density ◽  
Dna Transfer ◽  
Plant Genome ◽  
In Planta ◽  
Agrobacterium Mediated Transformation ◽  
The Third ◽  
Winter Bread Wheat ◽  
Inoculation Medium

Aim. Optimization of conditions for genetic transformation of new promising genotypes of winter bread wheat (T. aestivum L.) by in planta method. Methods. Agrobacterium-mediated transformation by in planta method using the strain AGL0 and vector construct pBi2E. Results. The influence of air temperature, optical density of cells of agrobacterial suspension, inoculation day and composition of inoculation medium on the frequency of obtaining transgenic plants of new winter wheat genotypes was studied. The dependence of the frequency obtaining of transgenic plants from environmental conditions, in particular temperature, has been established. It was found that the temperature regime of 20-22°C provided the largest number (4.8%) of wheat transformants, and when the temperature is reduced to 16-18°C there is a decrease in the efficiency of T-DNA transfer into the plant genome and the lowest frequency of transformation (0.7%). Conclusions. The largest number of transformants was obtained using a inoculation medium without sucrose, the optical density of cells of the agrobacterial suspension of 0.6 op.od. and inoculation on the third day after castration of ears. Keywords: T. aestivum, Agrobacterium-mediated transformation in planta, optimization of conditions.

Agrobacterium-mediated in planta transformation of maize germ cells

2010 ◽  
Vol 46 (4) ◽  
pp. 501-504 ◽  
Author(s):  
E. M. Mamontova ◽  
V. A. Velikov ◽  
I. V. Volokhina ◽  
M. I. Chumakov
Keyword(s):  
Germ Cells ◽  
In Planta Transformation ◽  
In Planta ◽  
Maize Germ
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