Engineered ectopic expression of the psbA gene encoding the photosystem II D1 protein in Synechocystis sp. PCC6803

2007 ◽  
Vol 92 (3) ◽  
pp. 315-325 ◽  
Author(s):  
Madhavi Kommalapati ◽  
Hong Jin Hwang ◽  
Hong-Liang Wang ◽  
Robert L. Burnap
Keyword(s):  
Photosystem Ii ◽  
Ectopic Expression ◽  
D1 Protein ◽  
Psba Gene ◽  
Gene Encoding ◽  
Synechocystis Sp

A New Ioxynil-Resistant Mutant in Synechocystis PCC 6714: Hypothesis on the Interaction of Ioxynil with the D1 Protein

1990 ◽  
Vol 45 (5) ◽  
pp. 436-440 ◽  
Author(s):  
S. Creuzet ◽  
G. Ajlani ◽  
C. Vernotte ◽  
C. Astier
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
D1 Protein ◽  
Resistant Mutant ◽  
Hydroxyl Group ◽  
Amino Acid Substitutions ◽  
Psba Gene ◽  
Molecular Models ◽  
Gene Encoding ◽  
Synechocystis Pcc 6714

A new Synechocystis 6714 mutant, loxIIA, resistant to the phenol-type herbicide ioxynil was isolated and characterized. The mutation found in the psbA gene (encoding the D1 photosystem II protein) is at the same codon 266 as for the first ioxynil-resistant mutant IoxIA previously selected [G. Ajlani. I. Meyer, C. Vernotte. and C. Astier, FEBS Lett. 246, 207-210 (1989)]. In IoxIIA, the change of Asn 266 to Asp gives a 3 × resistance, whereas in IoxIA, the change of the same amino acid to Thr gives a 10 × resistance. The effect of these different amino acid substitutions on the ioxynil resistance phenotype has allowed us to construct molecular models and calculate the hydrogen-bonding energies between the hydroxyl group of ioxynil and the respective amino acids at position 266.

Heteroplasmy and atrazine resistance in Chenopodium album and Senecio vulgaris

2016 ◽  
Vol 71 (7-8) ◽  
pp. 267-272
Author(s):  
Michaela Bühler ◽  
Arno Bogenrieder ◽  
Heinrich Sandermann ◽  
Dieter Ernst
Keyword(s):  
Photosystem Ii ◽  
Chenopodium Album ◽  
D1 Protein ◽  
Base Substitution ◽  
Individual Plant ◽  
Chloroplast Gene ◽  
Gene Encoding ◽  
Senecio Vulgaris ◽  
Different Types ◽  
Binding Sequence

Abstract Atrazine-resistant weeds are well known, and the resistance is primarily caused by a point mutation in the psbA chloroplast gene encoding the photosystem II D1 protein. Heteroplasmy, the presence of different types of chloroplasts in an individual plant, is also very common. Thus, atrazine-resistant weeds may also partly possess the atrazine-binding sequence and vice versa. The region of the psbA gene containing the mutation was sequenced from atrazine-resistant and atrazine-sensitive Chenopodium album and Senecio vulgaris plants. In atrazine-sensitive C. album plants, the expected AGT triplet was found. The atrazine-resistant plants contained the expected base substitution (AGT to GGT); however, in addition the AGT triplet was found. The atrazine-resistant S. vulgaris plants contained the expected GGT sequence, whereas the atrazine-sensitive plants contained both the AGT and GGT sequences. This clearly indicates that in addition to Gly264 also Ser264 is present in atrazine-resistant plants, and vice versa in atrazine-sensitive plants, indicating heteroplasmy in these weeds.

scholarly journals The emergence of a atrazine resistant black nightshade (Solanum nigrum L.) biotype and molecular basis of the resistance

2010 ◽  
Vol 40 (No. 3) ◽  
pp. 94-100 ◽  
Author(s):  
J. Salava ◽  
D. Chodová ◽  
K. Nováková
Keyword(s):  
Chlorophyll Fluorescence ◽  
Photosystem Ii ◽  
Rapid Detection ◽  
Molecular Basis ◽  
Restriction Analysis ◽  
D1 Protein ◽  
Solanum Nigrum ◽  
Gene Encoding ◽  
Black Nightshade ◽  
Pcr Products

Seeds from atrazine resistant plants of black nightshade (<I>Solanum nigrum </I>L.)<I> </I>were collected at the railway station Prague-Vršovice, seeds from susceptible plants in Vyšehořovice (Prague East district). Tests on emergence showed that in both resistant and susceptible biotypes it was highest at a seeding depth of 1 mm, and that at the same seeding depth there were statistically significant differences in emergence between the resistant and susceptible biotypes. The resistance or susceptibility to atrazine was tested by both a chlorophyll fluorescence assay and spraying with atrazine. A region of the gene encoding D1 protein of photosystem II was sequenced and compared between the resistant and susceptible biotypes. Resistance to atrazine in the <I>S. nigrum</I> biotype from Vršovice was conferred by a glycine for serine substitution at residue 264 of the D1 protein. In the plants of the biotypes there was excellent correspondence between the presence of the mutation and herbicide resistance. The assay based on restriction analysis of PCR products can be used for rapid detection of the mutation in populations of black nightshade.

Sign in / Sign up

Export Citation Format

Share Document