Superoxide Dismutase Activity in Mesocarp Tissue from Divergent Cucumis melo L. Genotypes

2009 ◽  
Vol 64 (3) ◽  
pp. 205-211 ◽  
Author(s):  
Gene E. Lester ◽  
John L. Jifon ◽  
Kevin M. Crosby
2004 ◽  
Vol 94 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Ioannis Vouldoukis ◽  
Dominique Lacan ◽  
Caroline Kamate ◽  
Philippe Coste ◽  
Alphonse Calenda ◽  
...  

2017 ◽  
Vol 9 (4) ◽  
pp. 2165-2169 ◽  
Author(s):  
Sandeep Kaur ◽  
Namarta Gupta

Effect of different seed treatments hydration, warm water, proline (10mM and 20mM) and salicylic acid (0.1mM and 0.5mM) was investigated on percent germination and activity of antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) at different temperatures in Muskmelon (Cucumis melo L.) seeds. It was observed that lower temperature (20˚C) had decreased seed germination and activity of various anti-oxidant enzymes. Various seed treatments increased percent germination and activity of these enzymes as com-pared to control at both the temperatures. Proline 20mM (96.6) and SA 0.1mM (91.6) showed better results as com-pared to proline 10mM (95.0) and salicylic acid 0.5mM (86.6) respectively. Thus, proline and salicylic acid play an ameliorating role on low temperature stress by enhancing the activities of antioxidant enzymes and scavenging the free radicals.


2016 ◽  
Vol 46 (3) ◽  
pp. 336-342 ◽  
Author(s):  
Daniel Teixeira Pinheiro ◽  
Aparecida Leonir da Silva ◽  
Laércio Junio da Silva ◽  
Marcelo Coelho Sekita ◽  
Denise Cunha Fernandes dos Santos Dias

ABSTRACT Salinity influences all germination stages and may adversely affect the seedlings establishment in the field. This study aimed at assessing the effects of salt stress on the physiological potential of melon (Cucumis melo L.) seeds. Germination and activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) enzymes were assessed at the osmotic potentials of 0.0 MPa, -0.1 MPa, -0.2 MPa, -0.3 MPa, -0.4 MPa and -0.5 MPa. The enzymatic activity was assessed after 24 h, 48 h and 72 h of imbibition. Seed germination was zero at -0.5 MPa and 91 % and 85 % at -0.1 MPa and -0.2 MPa, respectively. In general, the activity of SOD, CAT and POX enzymes increased with the salt-induced stress, in the different times of imbibition, and these increases were more evident at -0.4 MPa and -0.5 MPa. The salt stress induced by NaCl diminishes the germination rate of melon seeds, particularly from the osmotic potential of -0.2 MPa. There is a significant increase in the activity of SOD, CAT and POX enzymes, mainly after 48 h of exposure.


2020 ◽  
Vol 13 (1) ◽  
pp. 76-83
Author(s):  
Aline Maria Brito Lucas ◽  
Joana Varlla de Lacerda Alexandre ◽  
Maria Thalyne Silva Araújo ◽  
Cicera Edna Barbosa David ◽  
Yuana Ivia Ponte Viana ◽  
...  

Background: Cardiac hypertrophy involves marked wall thickening or chamber enlargement. If sustained, this condition will lead to dysfunctional mitochondria and oxidative stress. Mitochondria have ATP-sensitive K+ channels (mitoKATP) in the inner membrane that modulate the redox status of the cell. Objective: We investigated the in vivo effects of mitoKATP opening on oxidative stress in isoproterenol- induced cardiac hypertrophy. Methods: Cardiac hypertrophy was induced in Swiss mice treated intraperitoneally with isoproterenol (ISO - 30 mg/kg/day) for 8 days. From day 4, diazoxide (DZX - 5 mg/kg/day) was used in order to open mitoKATP (a clinically relevant therapy scheme) and 5-hydroxydecanoate (5HD - 5 mg/kg/day) or glibenclamide (GLI - 3 mg/kg/day) were used as mitoKATP blockers. Results: Isoproterenol-treated mice had elevated heart weight/tibia length ratios (HW/TL). Additionally, hypertrophic hearts had elevated levels of carbonylated proteins and Thiobarbituric Acid Reactive Substances (TBARS), markers of protein and lipid oxidation. In contrast, mitoKATP opening with DZX avoided ISO effects on gross hypertrophic markers (HW/TL), carbonylated proteins and TBARS, in a manner reversed by 5HD and GLI. Moreover, DZX improved mitochondrial superoxide dismutase activity. This effect was also blocked by 5HD and GLI. Additionally, ex vivo treatment of isoproterenol- induced hypertrophic cardiac tissue with DZX decreased H2O2 production in a manner sensitive to 5HD, indicating that this drug also acutely avoids oxidative stress. Conclusion: Our results suggest that diazoxide blocks oxidative stress and reverses cardiac hypertrophy. This pharmacological intervention could be a potential therapeutic strategy to prevent oxidative stress associated with cardiac hypertrophy.


Author(s):  
César Elías Baquero Maestre ◽  
Ángela Arcila Cardona ◽  
Heriberto Arias Bonilla ◽  
Marlon Yacomelo Hernández
Keyword(s):  

ChemInform ◽  
2011 ◽  
Vol 42 (8) ◽  
pp. no-no
Author(s):  
Gene E. Lester ◽  
John L. Jifon ◽  
Donald J. Makus

Sign in / Sign up

Export Citation Format

Share Document