Affinity characteristics of anti-β-(1→3)-d-glucan monoclonal antibody 3G11 by fluorescence polarization immunoassay

2021 ◽  
Vol 70 (5) ◽  
pp. 975-981
Author(s):  
L. I. Mukhametova ◽  
V. B. Krylov ◽  
A. S. Solovev ◽  
D. V. Yashunsky ◽  
A. L. Matveev ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Fluorescence Polarization ◽  
Fluorescence Polarization Immunoassay

Monoclonal Fluorescence Polarization Immunoassay Evaluated for Monitoring Cyclosporine in Whole Blood After Kidney, Heart, and Liver Transplantation

1992 ◽  
Vol 38 (1) ◽  
pp. 123-126 ◽  
Author(s):  
M Winkler ◽  
G Schumann ◽  
D Petersen ◽  
M Oellerich ◽  
K Wonigeit
Keyword(s):  
Monoclonal Antibody ◽  
Liver Transplantation ◽  
Whole Blood ◽  
Fluorescence Polarization ◽  
Fluorescence Polarization Immunoassay ◽  
Transplant Patients ◽  
Specific Radioimmunoassay ◽  
Metabolite Concentrations ◽  
A Prospective Study

Abstract In a prospective study we evaluated a novel fluorescence polarization immunoassay (FPIA) for determining cyclosporine (CsA) in whole blood. FPIA uses a monoclonal antibody and is performed on the TDx (Abbott). The within-series (CV less than 2%) and between-days (CV less than 3.3%) precision of the assay was excellent. The results obtained by the monoclonal FPIA in samples from transplant patients (n = 100) averaged 31.9% and 20.2% higher than those by HPLC and a specific radioimmunoassay (INCStar), respectively. Results by all three methods correlated well. Follow-up studies during the early course after liver transplantation, however, suggested that high metabolite concentrations affect FPIA results. This is explained by previously described cross-reactions of the monoclonal antibody with some CsA metabolites. The FPIA results in samples of such patients should be interpreted cautiously.

Fluorescence polarization immunoassay based on a new monoclonal antibody for the detection of the Diisobutyl phthalate in Yoghurt

Food Control ◽  
2019 ◽  
Vol 105 ◽  
pp. 38-44 ◽  
Author(s):  
Yingshan Chen ◽  
Qiyi He ◽  
Ding Shen ◽  
Zhengyun Jiang ◽  
Sergei A. Eremin ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Fluorescence Polarization ◽  
Fluorescence Polarization Immunoassay

Monoclonal Antibody-Based Fluorescence Polarization Immunoassay for Sulfamethoxypyridazine and Sulfachloropyridazine

2007 ◽  
Vol 55 (17) ◽  
pp. 6871-6878 ◽  
Author(s):  
Zhanhui Wang ◽  
Suxia Zhang ◽  
Irina S. Nesterenko ◽  
Sergei A. Eremin ◽  
Jianzhong Shen
Keyword(s):  
Monoclonal Antibody ◽  
Fluorescence Polarization ◽  
Fluorescence Polarization Immunoassay
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