Elicitor-enhanced production of gymnemic acid in cell suspension cultures of Gymnema sylvestre R. Br.

2011 ◽  
Vol 108 (1) ◽  
pp. 27-35 ◽  
Author(s):  
V. Veerashree ◽  
C. M. Anuradha ◽  
Vadlapudi Kumar
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Gymnema Sylvestre ◽  
Gymnemic Acid ◽  
Enhanced Production

scholarly journals Elicitation of gymnemic acid production in cell suspension cultures of Gymnema sylvestre R.Br. through endophytic fungi

3 Biotech ◽  
2016 ◽  
Vol 6 (2) ◽  
Author(s):  
Vasudeva Reddy Netala ◽  
Venkata Subbaiah Kotakadi ◽  
Susmila Aparna Gaddam ◽  
Sukhendu Bikash Ghosh ◽  
Vijaya Tartte
Keyword(s):  
Cell Suspension ◽  
Endophytic Fungi ◽  
Acid Production ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Gymnema Sylvestre ◽  
Gymnemic Acid

Establishment of Callus and Cell Suspension Cultures of Corydalis saxicola Bunting, a Rare Medicinal Plant

2006 ◽  
Vol 61 (3-4) ◽  
pp. 251-256 ◽  
Author(s):  
Hua Cheng ◽  
Long-Jiang Yu ◽  
Qiong-Yue Hu ◽  
Shan-Cai Chen ◽  
You-Ping Sun
Keyword(s):  
Cell Suspension ◽  
Callus Induction ◽  
Large Scale ◽  
Callus Formation ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Biomass Accumulation ◽  
Suspension Cultures ◽  
Inoculum Size ◽  
Enhanced Production

An efficient procedure has been developed for callus induction and cell suspension cultures of C. saxicola for the first time. Explant selection was carried out among leaf, stem and root to select a suitable type of explants capable of higher callus formation. Leaf explants thus selected showed maximum response to callus induction (67.1%). Modified B5 medium supplemented with 0.5 mg l−1 2,4-D plus 2 mg l−1 BA was the most favorable medium for callus formation with the highest induction rate (94.8%) and greatest fresh weight of callus (1.7 g per explant). Cell suspension cultures were established by transferring 2-8 g fresh callus to 80 ml liquid B5 medium. An inoculum size of 8 g produced the greatest biomass accumulation, dehydrocavidine and berberine productions, which was 13.1 g l−1, 8.0 mg l−1 and 4.1 mg l−1, respectively. In response to various sucrose concentrations from 10 g l−1 to 80 g l−1, cultures with 60 g sucrose l−1 not only produced the highest dry biomass (18.5 g l−1) but also the highest formation of dehydrocavidine (11.6 mg l−1) and berberine (7.6 mg l−1). These prepared cell suspension cultures provided a useful material for further regulation of alkaloid biosynthesis and for enhanced production of valuable alkaloids on a large scale.

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