In vitro production of gymnemic acid from cell suspension cultures of Gymnema sylvestre R. Br

2011 ◽  
Vol 11 (5) ◽  
pp. 537-540 ◽  
Author(s):  
Praveen Nagella ◽  
Ill-Min chung ◽  
Hosakatte Niranjana Murthy
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Gymnema Sylvestre ◽  
In Vitro Production ◽  
Gymnemic Acid ◽  
Vitro Production

Production of Limonoids Through Callus and Cell Suspension Cultures of Chinaberry (Melia Azedarach L.)

2021 ◽  
Vol 50 (2) ◽  
pp. 301-309
Author(s):  
Nadir Ali Rind ◽  
Khalid Hussain Rind ◽  
Muhammad Umar Dahot ◽  
Hafiza Faiza ◽  
ÖZlem Aksoy ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Callus Induction ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Melia Azedarach ◽  
In Vitro Production ◽  
Callus Cell ◽  
Uv Visible ◽  
Vitro Production

To study the in vitro production of limonoid contents through callus and cell suspension cultures of chinaberry (Melia azedarach L.) in different explants were inoculated in Murashige and Skoog (MS) medium for the callus induction with various plant growth regulators (PGRs) separately as well as in combinations. The highest callus induction (73.3%) was observed in nodular stem sections and further callus was subcultured for multiplication and finally transferred to cell suspension medium. The optimized parameters for the production of total limonoids were adjusted and UV-visible spectrophotometer was used to quantify total limonoids at 577 nm. Production of total limonoids through callus cell suspension cultures on the optimized medium was highest (141.7 μg/ml) followed by other sources like KH2PO4 (0.1 g/l) extra supplementation with optimized medium produced (138 μg/ml). Plant callus cell suspension cultures through optimized medium may be considered as a good source for the production of bio-products and its purified form could be used as a medicinal sources. Bangladesh J. Bot. 50(2): 301-309, 2021 (June)

scholarly journals Elicitation of gymnemic acid production in cell suspension cultures of Gymnema sylvestre R.Br. through endophytic fungi

3 Biotech ◽  
2016 ◽  
Vol 6 (2) ◽  
Author(s):  
Vasudeva Reddy Netala ◽  
Venkata Subbaiah Kotakadi ◽  
Susmila Aparna Gaddam ◽  
Sukhendu Bikash Ghosh ◽  
Vijaya Tartte
Keyword(s):  
Cell Suspension ◽  
Endophytic Fungi ◽  
Acid Production ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Gymnema Sylvestre ◽  
Gymnemic Acid

scholarly journals Establishment of Callus and Cell Suspension Cultures of Helicteres isora L.

2018 ◽  
pp. 01-07 ◽  
Author(s):  
Samrin Shaikh ◽  
Varsha Shriram ◽  
Tushar Khare and Vinay Kumar
Keyword(s):  
Cell Suspension ◽  
Wide Spectrum ◽  
Scale Up ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Ms Medium ◽  
In Vitro Production ◽  
Helicteres Isora ◽  
Therapeutic Activities

Helicteres isora L. (Malvaceae) is a medicinal plant highly used in traditional therapeutic practices. It has shown wide-spectrum therapeutic activities including anti-plasmodial, hypoglycemic, hypolipidemic, hepatoprotective, antinociceptive, antioxidant and anti-HIV. Present investigation was undertaken with an objective of establishment of cell suspension cultures of this plant which can be further used for in vitro production of desired secondary metabolites and their further scale-up. Seed dormancy was broken using sulfuric acid and seedlings were raised in vitro. MS medium supplemented with 2,4-D (0.5 mg/L) produced maximum callus from the nodal explants. The callus produced was used as an explant for the establishment of suspension cultures. MS medium without any supplement was proved best for the establishment of cell suspension cultures of H. isora. To the best of our knowledge, this is the first report on H. isora cell suspension culture establishment.    

scholarly journals In vitro production of sesquiterpenes by electro-elicitation in Gyrinops walla Gaetner calli and cell suspension cultures

2021 ◽  
Vol 5 (2) ◽  
pp. 70-78
Author(s):  
D. Sachithri P. Munasinghe ◽  
Seneviratnege Somaratne ◽  
Shyama R. Weerakoon ◽  
Chandani Ranasinghe
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Global Market ◽  
Cell Suspensions ◽  
Sri Lankan ◽  
Sustainable Utilization ◽  
In Vitro Production ◽  
Ancient Times ◽  
Vitro Production

Abstract Aloeswood or Agarwood has long been perfumery commodity traded between Mediterranean Region and Southeast Asia since ancient times. Oil or chips are obtained by destructive harvesting of several Thymeleaceous genera includes Aquilaria and Gonystylus and are expensive and highly demanded ingredients in the global market. The recent recovery of Gyrinops walla as a potential producer of market-quality agarwood in mature damaged woods and branches, the intense illicit felling and exportation G. walla leading to the verge of extinction from Sri Lankan flora. The sustainable utilization of G. walla undoubtedly enhances the foreign exchange of the country and the non-destructive utilization G. walla through biotechnology is the only option available for sustainable exploitation and conservation of the vulnerable G. walla species. An electro-elicitation apparatuses were designed and built to stimulate calli and cell suspensions. The elicited and calli and cell suspensions were harvested at different time periods to extract sesquiterpenes. Current intensities of 10 mA and 50 mA, and 0.1 mA were the optimum values for the induction of sesquiterpenes; γ-selinene, β-caryophyllene, α-cadinol and α-guaiene, production in both calli and cell suspension after 8 weeks and 6 hours, respectively. The findings of the study led to conclude the possibility of induction of production of sesquiterpenes through electro-elicitation of G. walla calli and cell suspension in an in vitro system for sustainable utilization and conservation endeavours.

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