From cells to embryos to rooted plantlets in a mist bioreactor

2013 ◽  
Vol 116 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Liwen Fei ◽  
Pamela J. Weathers
Keyword(s):  
Mist Bioreactor

scholarly journals Biomass production of hairy roots of Artemisia annua and Arachis hypogaea in a scaled-up mist bioreactor

2010 ◽  
Vol 107 (5) ◽  
pp. 802-813 ◽  
Author(s):  
Ganapathy Sivakumar ◽  
Chunzhao Liu ◽  
Melissa J. Towler ◽  
Pamela J. Weathers
Keyword(s):  
Arachis Hypogaea ◽  
Biomass Production ◽  
Hairy Roots ◽  
Artemisia Annua ◽  
Mist Bioreactor

Development of a nontoxic acoustic window nutrient-mist bioreactor and relevant growth data

1996 ◽  
Vol 32 (4) ◽  
pp. 299-304 ◽  
Author(s):  
C. S. Buer ◽  
M. J. Correll ◽  
T. C. Smith ◽  
M. J. Towler ◽  
P. J. Weathers ◽  
...  
Keyword(s):  
Growth Data ◽  
Acoustic Window ◽  
Mist Bioreactor

Development of a nutrient mist bioreactor for growth of hairy roots

1999 ◽  
Vol 35 (3) ◽  
pp. 271-274 ◽  
Author(s):  
C. Z. Liu ◽  
Y. C. Wang ◽  
B. Zhao ◽  
C. Guo ◽  
F. Ouyang ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Mist Bioreactor

scholarly journals The growth and saponin production of Platycodon grandiflorum (Jacq.) A. DC. (Chinese bellflower) hairy roots cultures maintained in shake flasks and mist bioreactor

2014 ◽  
Vol 83 (3) ◽  
pp. 229-237 ◽  
Author(s):  
Natalia Urbańska ◽  
Joanna Giebułtowicz ◽  
Olga Olszowska ◽  
Wojciech J. Szypuła
Keyword(s):  
Hairy Roots ◽  
Woody Plant ◽  
Dry Weight ◽  
Biomass Growth ◽  
Sucrose Uptake ◽  
Platycodon Grandiflorum ◽  
Mist Bioreactor ◽  
Woody Plant Medium ◽  
Transgenic Hairy Roots ◽  
Shake Flasks

The growth and saponin accumulation were measured in two lines of transgenic hairy roots of <em>Platycodon grandiflorum</em>, Pl 6 and Pl 17, cultured for 8 weeks in 250-ml shake flasks containing 50 ml of hormone-free woody plant medium supplemented with 40 g/l sucrose and in the Pl 17 line cultured for 12 weeks in a 5-l mist bioreactor containing 1.5 l of the same medium. With both methods, the growth of transgenic hairy roots was assessed as both fresh and dry weight and the biomass growth was correlated with the conductivity and sucrose uptake. The accumulation of saponins was measured and compared with that in roots derived from the field cultivation. The saponin concentrations were significantly higher in the two hairy root lines cultured in shake flasks [6.92 g/100 g d.w. (g%) and 5.82 g% in Pl 6 and Pl 17, respectively] and the line cultured in the bioreactor (5.93 g%) than in the roots derived from the field cultivation (4.02 g%). The results suggest that cultures of <em>P. grandiflorum</em> hairy roots may be a valuable source for obtaining saponins.

Production of artemisinin by shoot cultures of Artemisia annua L. in a modified inner-loop mist bioreactor

Plant Science ◽  
1998 ◽  
Vol 135 (2) ◽  
pp. 211-217 ◽  
Author(s):  
Chun-Zhao Liu ◽  
Yu-Chun Wang ◽  
Chen Guo ◽  
Fan Ouyang ◽  
He-Chun Ye ◽  
...  
Keyword(s):  
Artemisia Annua ◽  
Shoot Cultures ◽  
Artemisia Annua L ◽  
Mist Bioreactor
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