Detection and assessment of risk factors associated with natural concurrent infection of Trypanosoma evansi and Anaplasma marginale in dairy animals by duplex PCR in eastern Punjab

2014 ◽  
Vol 47 (1) ◽  
pp. 251-257 ◽  
Author(s):  
Amrita Sharma ◽  
Lachhman Das Singla ◽  
Ashuma Tuli ◽  
Paramjit Kaur ◽  
Mandeep Singh Bal
2015 ◽  
Vol 40 (4) ◽  
pp. 1359-1364 ◽  
Author(s):  
Abhishek Gupta ◽  
N. K. Singh ◽  
Harkirat Singh ◽  
S. S. Rath

2018 ◽  
Vol 67 (6) ◽  
pp. 659-665 ◽  
Author(s):  
Shola David Ola-Fadunsin ◽  
Fufa Ido Gimba ◽  
Donea Abdurazak Abdullah ◽  
Reuben Sunil Kumar Sharma ◽  
Faez Jesse Firdaus Abdullah ◽  
...  

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Amrita Sharma ◽  
Lachhman Das Singla ◽  
Ashuma Tuli ◽  
Paramjit Kaur ◽  
Balwinder Kaur Batth ◽  
...  

Specific duplex polymerase chain reaction (PCR) was employed on 411 (386 cattle and 25 buffaloes) blood samples of dairy animals from 9 districts of Punjab, India, for simultaneous detection ofBabesia bigeminaandTrypanosoma evansi. The results were compared and correlated with conventional Giemsa stained thin blood smear (GSTBS) examination and haematological alterations to know the clinical status and pathogenicity of infections. The Bg3/Bg4 and TR3/TR4 primers were used in duplex PCR forB. bigeminaandT. evansiamplified products of 689 bp and 257 bp, respectively. The overall prevalence by duplex PCR was found to be 36.49, 2.43, and 3.41% forT. evansi,B. bigemina, and dual infection, respectively. A more significant difference was observed for dual infection status (P≤0.005) as compared toT. evansi(P≤0.05) andB. bigemina(P≤0.01) among various districts under study. A very low prevalence ofT. evansi(0.73%) andB. bigemina(0.48%) was seen by GSTBS. The highly sensitive, specific, and cost-effective duplex PCR was able to detect latentT. evansiandB. bigeminainfection in cattle and buffaloes. Haematological evaluation revealed marked pathology inB. bigeminainfected group and in dual infected group in contrast to that infected withT. evansialone.


2014 ◽  
Author(s):  
Ariel M. Barber ◽  
Alexandra Crouch ◽  
Stephen Campbell

1992 ◽  
Vol 68 (03) ◽  
pp. 261-263 ◽  
Author(s):  
A K Banerjee ◽  
J Pearson ◽  
E L Gilliland ◽  
D Goss ◽  
J D Lewis ◽  
...  

SummaryA total of 333 patients with stable intermittent claudication at recruitment were followed up for 6 years to determine risk factors associated with subsequent mortality. Cardiovascular diseases were the underlying cause of death in 78% of the 114 patients who died. The strongest independent predictor of death during the follow-up period was the plasma fibrinogen level, an increase of 1 g/l being associated with a nearly two-fold increase in the probability of death within the next 6 years. Age, low ankle/brachial pressure index and a past history of myocardial infarction also increased the probability of death during the study period. The plasma fibrinogen level is a valuable index of those patients with stable intermittent claudication at high risk of early mortality. The results also provide further evidence for the involvement of fibrinogen in the pathogenesis of arterial disease.


2013 ◽  
Author(s):  
Giovanni Corona ◽  
Giulia Rastrelli ◽  
Emmanuele Jannini ◽  
Linda Vignozzi ◽  
Edoardo Mannucci ◽  
...  

2019 ◽  
Author(s):  
Claire Beynon ◽  
Nora Pashyan ◽  
Elizabeth Fisher ◽  
Dougal Hargreaves ◽  
Linda Bailey ◽  
...  

2015 ◽  
Vol 18 (1) ◽  
pp. 006
Author(s):  
Hasan Reyhanoglu ◽  
Kaan Ozcan ◽  
Murat Erturk ◽  
Fatih İslamoglu ◽  
İsa Durmaz

<strong>Objective:</strong> We aimed to evaluate the risk factors associated with acute renal failure in patients who underwent coronary artery bypass surgery.<br /><strong>Methods:</strong> One hundred and six patients who developed renal failure after coronary artery bypass grafting (CABG) constituted the study group (RF group), while 110 patients who did not develop renal failure served as a control group <br />(C group). In addition, the RF group was divided into two subgroups: patients that were treated with conservative methods without the need for hemodialysis (NH group) and patients that required hemodialysis (HR group). Risk factors associated with renal failure were investigated.<br /><strong>Results:</strong> Among the 106 patients that developed renal failure (RF), 80 patients were treated with conservative methods without any need for hemodialysis (NH group); while <br />26 patients required hemodialysis in the postoperative period (HR group). The multivariate analysis showed that diabetes mellitus and the postoperative use of positive inotropes and adrenaline were significant risk factors associated with development of renal failure. In addition, carotid stenosis and postoperative use of adrenaline were found to be significant risk factors associated with hemodialysis-dependent renal failure (P &lt; .05). The mortality in the RF group was determined as 13.2%, while the mortality rate in patients who did not require hemodialysis and those who required hemodialysis was 6.2% and 34%, respectively.<br /><strong>Conclusion:</strong> Renal failure requiring hemodialysis after CABG often results in high morbidity and mortality. Factors affecting microcirculation and atherosclerosis, like diabetes mellitus, carotid artery stenosis, and postoperative vasopressor use remain the major risk factors for the development of renal failure.<br /><br />


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