Functional characterization of βC1 gene of Cotton leaf curl Multan betasatellite

Virus Genes ◽  
2012 ◽  
Vol 46 (1) ◽  
pp. 111-119 ◽  
Author(s):  
Neha Tiwari ◽  
P. K. Sharma ◽  
V. G. Malathi
Keyword(s):  
Functional Characterization ◽  
Cotton Leaf ◽  
Leaf Curl

scholarly journals Functional Characterization of a Bidirectional Plant Promoter from Cotton Leaf Curl Burewala Virus Using an Agrobacterium-Mediated Transient Assay

Viruses ◽  
2014 ◽  
Vol 6 (1) ◽  
pp. 223-242 ◽  
Author(s):  
Muhammad Ashraf ◽  
Ahmad Shahid ◽  
Abdul Rao ◽  
Kamran Bajwa ◽  
Tayyab Husnain
Keyword(s):  
Functional Characterization ◽  
Transient Assay ◽  
Cotton Leaf ◽  
Plant Promoter ◽  
Leaf Curl

scholarly journals Functional Characterization of Coat Protein and V2 Involved in Cell to Cell Movement of Cotton Leaf Curl Kokhran Virus-Dabawali

PLoS ONE ◽  
2011 ◽  
Vol 6 (11) ◽  
pp. e26929 ◽  
Author(s):  
C. G. Poornima Priyadarshini ◽  
M. V. Ambika ◽  
R. Tippeswamy ◽  
H. S. Savithri
Keyword(s):  
Coat Protein ◽  
Functional Characterization ◽  
Cell Movement ◽  
Cotton Leaf ◽  
Leaf Curl

scholarly journals Cloning and Partial Characterization of Cotton Leaf Curl Burewala Virus From Khanewal

2021 ◽  
Vol 3 (1) ◽  
pp. 28-34
Author(s):  
Fizza Akhter ◽  
Muhammad Tahir
Keyword(s):  
Coat Protein ◽  
Complete Characterization ◽  
Universal Primers ◽  
Ammonium Bromide ◽  
Cotton Leaf ◽  
Cotton Production ◽  
Leaf Sample ◽  
Coat Protein Region ◽  
Leaf Curl

Begomoviruses are a serious threat to cotton production throughout the world. In Pakistan, enormous crop losses occur as a result of cotton leaf curl disease (CLCuD) caused by begomoviruses. Molecular characterization of begomoviruses has made possible the identification and analysis of begomoviruses prevalent in a host plant. Infected cotton leaf sample (C-59) was obtained from area around Khanewal during 2011. The total DNA was isolated from the infected sample by Cetyl trimethyl ammonium bromide (CTAB) method. An expected size band of approximately 1100bp, covering coat protein region of the virus, was amplified using universal primers. The amplified product was T/A cloned and sequenced to its entirety. DNA sequence showed 99% nucleotide sequence identity to each of Cotton leaf curl Burewala virus ((CLCuBuV; Accession No HF549Begomoviruses are a serious threat to cotton production throughout the world. In Pakistan, enormous crop losses occur as a result of cotton leaf curl disease (CLCuD) caused by begomoviruses. Molecular characterization of begomoviruses has made possible the identification and analysis of begomoviruses prevalent in a host plant. Infected cotton leaf sample (C-59) was obtained from area around Khanewal during 2011. The total DNA was isolated from the infected sample by Cetyl trimethyl ammonium bromide (CTAB) method. An expected size band of approximately 1100bp, covering coat protein region of the virus, was amplified using universal primers. The amplified product was T/A cloned and sequenced to its entirety. DNA sequence showed 99% nucleotide sequence identity to each of Cotton leaf curl Burewala virus ((CLCuBuV; Accession No HF549184)) and Cotton leaf curl Kokhran virus (CLCuKV; Accession No AJ002449)). Since CLCuBuV is a recombinant of CLCuKV and Cotton leaf curl Multan virus and the coat protein region of CLCuBuV was derived from CLCuKV that is most probable reason that the available sequence showed identity with CLCuBuV as well as CLCuKV. A complete characterization of full length virus will determine whether isolate C-59 is CLCuBuV or CLCuKV. Literature indicates that there is no existence of CLCuKV within the region and CLCuBuV is dominating within Indo-Pak184)) and Cotton leaf curl Kokhran virus (CLCuKV; Accession No AJ002449)). Since CLCuBuV is a recombinant of CLCuKV and Cotton leaf curl Multan virus and the coat protein region of CLCuBuV was derived from CLCuKV that is most probable reason that the available sequence showed identity with CLCuBuV as well as CLCuKV. A complete characterization of full length virus will determine whether isolate C-59 is CLCuBuV or CLCuKV. Literature indicates that there is no existence of CLCuKV within the region and CLCuBuV is dominating within Indo-Pak

scholarly journals Identification, Isolation and Characterization of GaCyPI Gene in Gossypium arboreum under Cotton Leaf Curl Virus Disease Stress

Phyton ◽  
2021 ◽  
Vol 90 (6) ◽  
pp. 1613-1632
Author(s):  
Zunaira Sher ◽  
Muhammad Umair Majid ◽  
Sameera Hassan ◽  
Fatima Batool ◽  
Beenish Aftab ◽  
...  
Keyword(s):  
Virus Disease ◽  
Cotton Leaf ◽  
Gossypium Arboreum ◽  
Isolation And Characterization ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Molecular and Biochemical Characterization of Cotton Epicuticular Wax in Defense Against Cotton Leaf Curl Disease

2015 ◽  
Vol 13 (4) ◽  
pp. 3-9
Author(s):  
Muhammad Azmat Ullah Khan ◽  
Ahmad Ali Shahid ◽  
Abdul Qayyum Rao ◽  
Kamran Shehzad Bajwa ◽  
Tahir Rehman Samiullah ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Epicuticular Wax ◽  
Cotton Leaf ◽  
Cotton Leaf Curl Disease ◽  
Leaf Curl Disease ◽  
Leaf Curl

Biochemical characterization of C4 protein of Cotton Leaf Curl Kokhran Virus-Dabawali

2013 ◽  
Vol 1830 (6) ◽  
pp. 3734-3744 ◽  
Author(s):  
Debojit Guha ◽  
C.G. Poornima Priyadarshini ◽  
Arunima Purakayastha ◽  
R. Thippeswamy ◽  
M. Lakshmikanth ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Cotton Leaf ◽  
Leaf Curl

Functional characterization of human brown adipose tissue metabolism

2020 ◽  
Vol 477 (7) ◽  
pp. 1261-1286 ◽  
Author(s):  
Marie Anne Richard ◽  
Hannah Pallubinsky ◽  
Denis P. Blondin
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Functional Characterization ◽  
Human Infants ◽  
Positron Emission ◽  
Brown Adipose ◽  
Treatment Of Obesity ◽  
And Function

Brown adipose tissue (BAT) has long been described according to its histological features as a multilocular, lipid-containing tissue, light brown in color, that is also responsive to the cold and found especially in hibernating mammals and human infants. Its presence in both hibernators and human infants, combined with its function as a heat-generating organ, raised many questions about its role in humans. Early characterizations of the tissue in humans focused on its progressive atrophy with age and its apparent importance for cold-exposed workers. However, the use of positron emission tomography (PET) with the glucose tracer [18F]fluorodeoxyglucose ([18F]FDG) made it possible to begin characterizing the possible function of BAT in adult humans, and whether it could play a role in the prevention or treatment of obesity and type 2 diabetes (T2D). This review focuses on the in vivo functional characterization of human BAT, the methodological approaches applied to examine these features and addresses critical gaps that remain in moving the field forward. Specifically, we describe the anatomical and biomolecular features of human BAT, the modalities and applications of non-invasive tools such as PET and magnetic resonance imaging coupled with spectroscopy (MRI/MRS) to study BAT morphology and function in vivo, and finally describe the functional characteristics of human BAT that have only been possible through the development and application of such tools.

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