Hepatitis B virus genotype A: design of reference sequences for sub-genotypes

Virus Genes ◽  
2016 ◽  
Vol 52 (3) ◽  
pp. 325-333 ◽  
Author(s):  
Qun Cai ◽  
Huilan Zhu ◽  
Yafei Zhang ◽  
Xu Li ◽  
Zhenhua Zhang
2001 ◽  
Vol 34 ◽  
pp. 15-16 ◽  
Author(s):  
J. Hou ◽  
R. Schilling ◽  
H.L.A. Janssen ◽  
R.A. Heijtink ◽  
R. Williams ◽  
...  

2005 ◽  
Vol 78 (1) ◽  
pp. 60-67 ◽  
Author(s):  
Mariko Kobayashi ◽  
Norio Akuta ◽  
Fumitaka Suzuki ◽  
Yoshiyuki Suzuki ◽  
Yasuji Arase ◽  
...  

2004 ◽  
Vol 78 (14) ◽  
pp. 7575-7581 ◽  
Author(s):  
Izumi Hasegawa ◽  
Yasuhito Tanaka ◽  
Anna Kramvis ◽  
Takanobu Kato ◽  
Fuminaka Sugauchi ◽  
...  

ABSTRACT The eight genotypes of hepatitis B virus (HBV) have different geographical distributions, virological characteristics, and clinical manifestations. A unique subtype of HBV genotype A (HBV/A) was reported in sub-Saharan Africa, raising the possibility that patients infected with this subtype (HBV/Aa [“a” for African and Asian]) may have different clinical outcomes than other HBV/A isolates (HBV/Ae [“e” for European]). Comparison between 30 HBV/Aa and 30 HBV/Ae isolates indicated that almost all HBV/Ae isolates had G at nucleotide (nt) 1809 and C at nt 1812, whereas HBV/Aa isolates had T1809/T1812. Taking advantage of these two single nucleotide polymorphisms (SNPs), a novel subtype-specific PCR assay in the X/precore/core region was developed. This assay was combined with a restriction fragment length polymorphism assay using BglII in a different region (nt 1984 to 1989), which has a SNP distinguishing HBV/Aa from HBV/Ae, resulting in 100% specificity for the combined assay. Application of the subtyping assay using sera from 109 paid donors in the United States indicated significantly different distributions of HBV/A subtypes among races; African-Americans, Caucasians, and Hispanics had HBV/Ae, whereas Asians had mainly HBV/Aa, suggesting that the HBV/Aa isolates may have been imported by recent immigration from Asia. In conclusion, the specificity and sensitivity of the combined subtyping assay were confirmed, and its usefulness was demonstrated in a practical context.


2011 ◽  
Vol 49 (3) ◽  
pp. 1017-1024 ◽  
Author(s):  
S. Fujisaki ◽  
Y. Yokomaku ◽  
T. Shiino ◽  
T. Koibuchi ◽  
J. Hattori ◽  
...  

2009 ◽  
Vol 154 (3) ◽  
pp. 525-529 ◽  
Author(s):  
Viviana Andrea Mbayed ◽  
Flavia Guadalupe Piñeiro y Leone ◽  
Silvana Claudia Pezzano ◽  
Rodolfo Héctor Campos

2015 ◽  
Vol 14 (4) ◽  
pp. 16521-16534 ◽  
Author(s):  
H.L. Zhu ◽  
C.T. Wang ◽  
J.B. Xia ◽  
X. Li ◽  
Z.H. Zhang

2021 ◽  
Author(s):  
Jonas Michel Wolf ◽  
Vagner Reinaldo Zingalli Bueno Pereira ◽  
Daniel Simon ◽  
Vagner Ricardo Lunge

2011 ◽  
Vol 17 (2) ◽  
pp. 272-277 ◽  
Author(s):  
Haruki Komatsu ◽  
Hidenori Sugawara ◽  
Ayano Inui ◽  
Eitaro Hiejima ◽  
Tsuyoshi Sogo ◽  
...  

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