scholarly journals Molecular characterization of the spike gene of the porcine epidemic diarrhea virus in Mexico, 2013–2016

Virus Genes ◽  
2017 ◽  
Vol 54 (2) ◽  
pp. 215-224 ◽  
Author(s):  
Rocío Lara-Romero ◽  
Luis Gómez-Núñez ◽  
José Luis Cerriteño-Sánchez ◽  
Laura Márquez-Valdelamar ◽  
Susana Mendoza-Elvira ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Porcine Epidemic Diarrhea Virus ◽  
Spike Gene ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Molecular characterization of the spike and ORF3 genes of porcine epidemic diarrhea virus in the Philippines

2016 ◽  
Vol 161 (5) ◽  
pp. 1323-1328 ◽  
Author(s):  
Yong Kwan Kim ◽  
Yoon-Young Cho ◽  
Byung-Hyun An ◽  
Seong-In Lim ◽  
Ji-Ae Lim ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Porcine Epidemic Diarrhea Virus ◽  
The Philippines ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Molecular Characterization of the ORF3 and S1 Genes of Porcine Epidemic Diarrhea Virus Non S-INDEL Strains in Seven Regions of China, 2015

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160561 ◽  
Author(s):  
Enyu Wang ◽  
Donghua Guo ◽  
Chunqiu Li ◽  
Shan Wei ◽  
Zhihui Wang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Characterization of a pathogenic full-length cDNA clone of a virulent porcine epidemic diarrhea virus strain AH2012/12 in China

Virology ◽  
2017 ◽  
Vol 500 ◽  
pp. 50-61 ◽  
Author(s):  
Baochao Fan ◽  
Zhengyu Yu ◽  
Fengjiao Pang ◽  
Xiangwei Xu ◽  
Baimeng Zhang ◽  
...  
Keyword(s):  
Virus Strain ◽  
Cdna Clone ◽  
Porcine Epidemic Diarrhea Virus ◽  
Full Length ◽  
Diarrhea Virus ◽  
Full Length Cdna ◽  
Porcine Epidemic Diarrhea

scholarly journals Emergence and evolution of highly pathogenic porcine epidemic diarrhea virus by natural recombination of a low pathogenic vaccine isolate and a highly pathogenic strain in the spike gene

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Huinan Wang ◽  
Libo Zhang ◽  
Yuanbin Shang ◽  
Rongrong Tan ◽  
Mingxiang Ji ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Intestinal Epithelial ◽  
Orf3 Gene ◽  
Highly Pathogenic ◽  
Spike Gene ◽  
Diarrhea Virus ◽  
Attenuated Vaccine ◽  
New Variant ◽  
Porcine Epidemic Diarrhea ◽  
Natural Recombination

Abstract Outbreaks of a new variant of porcine epidemic diarrhea virus (PEDV) at the end of 2010 have raised interest in the mutation and recombination of PEDV. A PEDV strain (CN/Liaoning25/2018) isolated from a clinical outbreak of piglet diarrhea contained a 49-bp deletion in the ORF3 gene. This deletion is considered a genetic characteristic of low pathogenic attenuated vaccine strains. However, CN/Liaoning25/2018 was highly pathogenic. Complete genome sequencing, identity analysis, phylogenetic tree construction, and recombination analysis showed that this virus was a recombinant strain containing the Spike (S) gene from the highly pathogenic CN/GDZQ/2014 strain and the remaining genomic regions from the low pathogenic vaccine isolate SQ2014. Histopathology and immunohistochemistry results confirmed that this strain was highly pathogenic and indicated that intestinal epithelial cell vacuolation was positively correlated with the intensity and density of PEDV antigens. A new natural recombination model for PEDV was identified. Our results suggest that new highly pathogenic recombinant strains in the field may be generated by recombination between low pathogenic attenuated live PEDV vaccines and pathogenic circulating PEDV strains. Our findings also highlight that the 49-bp deletion of the ORF3 gene in low pathogenic attenuated vaccine strains will no longer be a reliable standard to differentiate the classical vaccine attenuated from the field strains.

scholarly journals Characterization and pathogenicity of Vero cell-attenuated porcine epidemic diarrhea virus CT strain

2019 ◽  
Vol 16 (1) ◽  
Author(s):  
Yu Wu ◽  
Wei Li ◽  
Qingfeng Zhou ◽  
Qunhui Li ◽  
Zhichao Xu ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Clinical Symptoms ◽  
Vero Cells ◽  
Economic Losses ◽  
Protective Effects ◽  
Diarrhea Virus ◽  
Cytopathic Effects ◽  
Porcine Epidemic Diarrhea ◽  
Piglet Survival

Abstract Background Porcine epidemic diarrhea virus (PEDV) has caused enormous economic losses to the global pig industry. Currently available PEDV vaccine strains have limited protective effects against PEDV variant strains. Methods In this study, the highly virulent epidemic virus strain CT was serially passaged in Vero cells for up to 120 generations (P120). Characterization of the different passages revealed that compared with P10 and P64, P120 had a higher viral titer and more obvious cytopathic effects, thereby demonstrating better cell adaptability. Results Pathogenicity experiments using P120 in piglets revealed significant reductions in clinical symptoms, histopathological lesions, and intestinal PEDV antigen distribution; the piglet survival rate in the P120 group was 100%. Furthermore, whole-genome sequencing identified 13 amino acid changes in P120, which might be responsible for the attenuated virulence of P120. Conclusions Thus, an attenuated strain was obtained via cell passaging and that this strain could be used in preparing attenuated vaccines.

scholarly journals Isolation and Serial Propagation of Porcine Epidemic Diarrhea Virus in Cell Cultures and Partial Characterization of the Isolate.

1992 ◽  
Vol 54 (2) ◽  
pp. 313-318 ◽  
Author(s):  
Ko-ichi KUSANAGI ◽  
Hiroyoshi KUWAHARA ◽  
Tetsuo KATOH ◽  
Tetsuo NUNOYA ◽  
Yoshihisa ISHIKAWA ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Porcine Epidemic Diarrhea Virus ◽  
Partial Characterization ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea ◽  
Serial Propagation

scholarly journals Cloning and further sequence analysis of the spike gene of attenuated porcine epidemic diarrhea virus DR13

Virus Genes ◽  
2006 ◽  
Vol 35 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Seong-Jun Park ◽  
Dae-Sub Song ◽  
Gun-Woo Ha ◽  
Bong-Kyun Park
Keyword(s):  
Sequence Analysis ◽  
Porcine Epidemic Diarrhea Virus ◽  
Spike Gene ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Complete genome characterization of porcine epidemic diarrhea virus in Vietnam

2015 ◽  
Vol 160 (8) ◽  
pp. 1931-1938 ◽  
Author(s):  
Dam Thi Vui ◽  
To Long Thanh ◽  
Nguyen Tung ◽  
Anchalee Srijangwad ◽  
Thitima Tripipat ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Complete Genome ◽  
Genome Characterization ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea
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