Mass spectrometry-based proteomic analysis of potential infectious bursal disease virus VP3-interacting proteins in chicken embryo fibroblasts cells

Virus Genes ◽  
2021 ◽  
Author(s):  
Sun-Ting Ma ◽  
Yong-Shan Wang ◽  
Xiao-Li Wang ◽  
Xing-Xia Xia ◽  
Zhen-Wei Bi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Disease Virus ◽  
Proteomic Analysis ◽  
Chicken Embryo ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Interacting Proteins ◽  
Bursal Disease ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts

scholarly journals Growth and Replication of Infectious Bursal Disease Virus in the DF-1 Cell Line and Chicken Embryo Fibroblasts

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Kaliyaperumal Rekha ◽  
Chandran Sivasubramanian ◽  
Ill-Min Chung ◽  
Muthu Thiruvengadam
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Chicken Embryo ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Population Doubling ◽  
Cytopathic Effects ◽  
Bursal Disease ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts

Infectious bursal disease virus (IBDV) causes a highly contagious disease in young chicks and leads to significant economic losses in the poultry industry. To determine a suitable cell line for IBDV infection, replication, and growth kinetics of the virus, DF-1 cells and chicken embryo fibroblasts (CEF) were used. The population doubling per day (Pd/D) was found to be higher in DF-1 as compared to CEF cells. A suitable time of infection (TOI) was established for increased production of virus and greater infectivity titers. The DF-1 and CEF cells were found to be susceptible to infection by producing marked cytopathic effects (CPEs), and the growth curves of IBDV in DF-1 and CEF cells were evaluated by infectivity assay using tissue culture infectious dose (TCID50). The cytopathic effects of the virus in DF-1 and CEF cells were found to be similar, but higher viral titers were detected in the DF-1 cells as compared to CEF. Thus the DF-1 cell line had a higher growth potential and infectivity, which will be of advantage in vaccine production.

scholarly journals Infectious Bursal Disease Virus Changes the Potassium Current Properties of Chicken Embryo Fibroblasts

Virology ◽  
1998 ◽  
Vol 246 (2) ◽  
pp. 362-369 ◽  
Author(s):  
Holger Repp ◽  
Hermann Nieper ◽  
Henning J. Draheim ◽  
Andreas Koschinski ◽  
Hermann Müller ◽  
...  
Keyword(s):  
Disease Virus ◽  
Chicken Embryo ◽  
Infectious Bursal Disease Virus ◽  
Potassium Current ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts

Competitive replication of different genotypes of infectious bursal disease virus on chicken embryo fibroblasts

Virus Genes ◽  
2008 ◽  
Vol 39 (1) ◽  
pp. 46-52 ◽  
Author(s):  
Lixue Shi ◽  
Haibin Li ◽  
Guangpeng Ma ◽  
Jiyong Zhou ◽  
Lianlian Hong ◽  
...  
Keyword(s):  
Disease Virus ◽  
Chicken Embryo ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts

scholarly journals Replication of classical infectious bursal disease virus in the chicken embryo related cell line

2000 ◽  
Vol 29 (3) ◽  
pp. 213-217 ◽  
Author(s):  
T.C. Cardoso ◽  
P. Rahal ◽  
D. Pilz ◽  
M. C. B. Teixeira ◽  
C. W. Arns
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Chicken Embryo ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Related Cell ◽  
Bursal Disease
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