Over expression and purification of recombinant human myocilin

2007 ◽  
Vol 24 (6) ◽  
pp. 903-907 ◽  
Author(s):  
P. J. Eswari Pandaranayaka ◽  
J. Kanagavalli ◽  
S. R. Krishnadas ◽  
P. Sundaresan ◽  
S. Krishnaswamy
Keyword(s):  
Expression And Purification ◽  
Over Expression

scholarly journals High Level Expression and Purification of Atl, the Major Autolytic Protein ofStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Vineet K. Singh
Keyword(s):  
Staphylococcus Aureus ◽  
Structural Properties ◽  
Biofilm Formation ◽  
Structural Studies ◽  
Ofstaphylococcus Aureus ◽  
Expression And Purification ◽  
High Level Expression ◽  
Over Expression ◽  
Important Protein ◽  
High Level

Staphylococcus aureusis a major human and animal pathogen. Autolysins regulate the growth, turnover, cell lysis, biofilm formation, and the pathogenicity ofS. aureus. Atl is the major autolysin inS. aureus. The biochemical and structural studies of staphylococcal Atl have been limited due to difficulty in cloning, high level overexpression, and purification of this protein. This study describes successful cloning, high level over-expression, and purification of two forms of fully functional Atl proteins. These pure proteins can be used to study the functional and structural properties of this important protein.

scholarly journals Cloning, over-expression and purification ofPseudomonas aeruginosa murCencoding uridine diphosphateN-acetylmuramate: L-alanine ligase

2000 ◽  
Vol 183 (2) ◽  
pp. 281-288
Author(s):  
Ahmed El Zoeiby ◽  
François Sanschagrin ◽  
Josée Lamoureux ◽  
André Darveau ◽  
Roger C. Levesque
Keyword(s):  
Expression And Purification ◽  
Over Expression
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