Bacterioplankton community structure in a eutrophic lake in relation to water chemistry

ABSTRACT Community structure of bacterioplankton was studied during the major growth season for phytoplankton (April to October) in the epilimnion of a temperate eutrophic lake (Lake Plußsee, northern Germany) by using comparative 5S rRNA analysis. Estimates of the relative abundances of single taxonomic groups were made on the basis of the amounts of single 5S rRNA bands obtained after high-resolution electrophoresis of RNA directly from the bacterioplankton. Full-sequence analysis of single environmental 5S rRNAs enabled the identification of single taxonomic groups of bacteria. Comparison of partial 5S rRNA sequences allowed the detection of changes of single taxa over time. Overall, the whole bacterioplankton community showed two to eight abundant (>4% of the total 5S rRNA) taxa. A distinctive seasonal succession was observed in the taxonomic structure of this pelagic community. A rather-stable community structure, with seven to eight different taxonomic units, was observed beginning in April during the spring phytoplankton bloom. A strong reduction in this diversity occurred at the beginning of the clear-water phase (early May), when only two to four abundant taxa were observed, with one taxon dominating (up to 72% of the total 5S rRNA). The community structure during summer stagnation (June and July) was characterized by frequent changes of different dominating taxa. During late summer, a dinoflagellate bloom (Ceratium hirudinella) occurred, with Comamonas acidovorans (β-subclass of the classProteobacteria) becoming the dominant bacterial species (average abundance of 43% of the total 5S rRNA). Finally, the seasonal dynamics of the community structure of bacterioplankton were compared with the abundances of other major groups of the aquatic food web, such as phyto- and zooplankton, revealing that strong grazing pressure by zooplankton can reduce microbial diversity substantially in pelagic environments.

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Changes in Bacterioplankton Community Structure and Activity with Depth in a Eutrophic Lake as Revealed by 5S rRNA Analysis

Applied and Environmental Microbiology ◽

10.1128/aem.68.7.3606-3613.2002 ◽

2002 ◽

Vol 68 (7) ◽

pp. 3606-3613 ◽

Cited By ~ 15

Author(s):

Katja Dominik ◽

Manfred G. Höfle

Keyword(s):

Community Structure ◽

Eutrophic Lake ◽

5S Rrna ◽

Bacterioplankton Community ◽

Chemical Stratification ◽

Different Seasons ◽

Different Depths ◽

Taxonomic Groups ◽

Structure And Activity ◽

Rrna Sequences

ABSTRACT The community structure of bacterioplankton was studied at different depths (0 to 25 m) of a temperate eutrophic lake (Lake Plußsee in northern Germany) by using comparative 5S rRNA analysis. The relative amounts of taxonomic groups were estimated from 5S rRNA bands separated by high-resolution electrophoresis. Comparison of partial 5S rRNA sequences enabled detection of changes in single taxa over space and during seasons. Overall, the bacterioplankton community was dominated by 3 to 14 abundant (>4% of the total 5S rRNA) taxa. In general, the number of 5S rRNA bands (i.e., the number of bacterial taxa) decreased with depth. In the fall, when thermal stratification and chemical stratification were much more pronounced than they were in the spring, the correlation between the depth layers and the community structure was more pronounced. Therefore, in the fall each layer had its own community structure; i.e., there were different community structures in the epilimnion, the metalimnion, and the hypolimnion. Only three 5S rRNA bands were detected in the hypolimnion during the fall, and one band accounted for about 70% of the total 5S rRNA. The sequences of individual 5S rRNA bands from the spring and fall were different for all size classes analyzed except two bands, one of which was identified as Comamonas acidivorans. In the overall analysis of the depth profiles, the diversity in the epilimnion contrasted with the reduced diversity of the bacterioplankton communities in the hypolimnion, and large differences occurred in the composition of the epilimnion at different seasons except for generalists like C. acidivorans.

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LAND USE, WATER CHEMISTRY, AQUATIC VEGETATION, AND ZOOPLANKTON COMMUNITY STRUCTURE OF SHALLOW LAKES

Ecological Applications ◽

10.1890/04-1494 ◽

2005 ◽

Vol 15 (4) ◽

pp. 1191-1198 ◽

Cited By ~ 70

Author(s):

Stanley I. Dodson ◽

Richard A. Lillie ◽

Susan Will-Wolf

Keyword(s):

Land Use ◽

Community Structure ◽

Water Chemistry ◽

Shallow Lakes ◽

Aquatic Vegetation ◽

Zooplankton Community ◽

Zooplankton Community Structure

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Overland flow during a storm event strongly affects stream water chemistry and bacterial community structure

Aquatic Sciences ◽

10.1007/s00027-021-00839-y ◽

2021 ◽

Vol 84 (1) ◽

Author(s):

Huong T. Le ◽

Thomas Pommier ◽

Olivier Ribolzi ◽

Bounsamay Soulileuth ◽

Sylvain Huon ◽

...

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Water Chemistry ◽

Bacterial Community Structure ◽

Overland Flow ◽

Stream Water ◽

Storm Event ◽

Stream Water Chemistry

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Changes in the Bacterioplankton Community Structure from Southern Gulf of Mexico During a Simulated Crude Oil Spill at Mesocosm Scale

Microorganisms ◽

10.3390/microorganisms7100441 ◽

2019 ◽

Vol 7 (10) ◽

pp. 441 ◽

Cited By ~ 2

Author(s):

Sonia S. Valencia-Agami ◽

Daniel Cerqueda-García ◽

Sébastien Putzeys ◽

María Magdalena Uribe-Flores ◽

Norberto Ulises García-Cruz ◽

...

Keyword(s):

Community Structure ◽

Gulf Of Mexico ◽

Crude Oil ◽

Oil Spill ◽

Initial Time ◽

P Value ◽

Sequencing Analysis ◽

Hydrocarbonoclastic Bacteria ◽

Bacterioplankton Community ◽

Southern Gulf Of Mexico

The southern Gulf of Mexico (sGoM) is highly susceptible to receiving environmental impacts due to the recent increase in oil-related activities. In this study, we assessed the changes in the bacterioplankton community structure caused by a simulated oil spill at mesocosms scale. The 16S rRNA gene sequencing analysis indicated that the initial bacterial community was mainly represented by Gamma-proteobacteria, Alpha-proteobacteria, Flavobacteriia, and Cyanobacteria. The hydrocarbon degradation activity, measured as the number of culturable hydrocarbonoclastic bacteria (CHB) and by the copy number of the alkB gene, was relatively low at the beginning of the experiment. However, after four days, the hydrocarbonoclastic activity reached its maximum values and was accompanied by increases in the relative abundance of the well-known hydrocarbonoclastic Alteromonas. At the end of the experiment, the diversity was restored to similar values as those observed in the initial time, although the community structure and composition were clearly different, where Marivita, Pseudohongiella, and Oleibacter were detected to have differential abundances on days eight–14. These changes were related with total nitrogen (p value = 0.030 and r2 = 0.22) and polycyclic aromatic hydrocarbons (p value = 0.048 and r2 = 0.25), according to PERMANOVA. The results of this study contribute to the understanding of the potential response of the bacterioplankton from sGoM to crude oil spills.

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