In vitro evaluation of some bacterial isolates as biofertilizers and biocontrol agents against the second stage juveniles of Meloidogyne incognita

2010 ◽  
Vol 26 (12) ◽  
pp. 2249-2256 ◽  
Author(s):  
Muhammed E. El-Hadad ◽  
Magdi I. Mustafa ◽  
Shawky M. Selim ◽  
Ahmed E. A. Mahgoob ◽  
Tarek S. El-Tayeb ◽  
...  
2018 ◽  
Vol 7 (2) ◽  
pp. 141-145
Author(s):  
Gulwaiz Akhter ◽  
◽  
Tabreiz Ahmad Khan ◽  

Aqueous leaf extracts were utilized to assess the nematicidal or nematostatic property on second stage juvenile of Meloidogyne incognita. The juvenile were incubate at various concentration of leaf extract viz., 250, 500, 1000 and 2000 ppm. Corrected mortality using Abbot’s formula was recorded after 12, 24 and 48 hours respectively. Correlation coefficient (Pearson) was checked to explain the association between percentages mortality of juvenile with extract concentrations. Linear regression was used to denote concentration and rank dependent outcome of four aqueous plant leaves extracts on the second stage juvenile (J2) mortality. All leaf extracts were found to be nematicidal or nematostatic in property. Maximum juvenile mortality rate was recorded in Xanthium strumarium throughout the incubation period as followed by Acalypha indica, Argemone mexicana and Colocasia gigantean. Concentration depended effect of X. strumarium and C. gigantean proved maximum and minimum level when analyzed by values of regression and correlation. Aqueous leaves extracts of these aforementioned weeds give us an idea about nematicidal properties and therefore may be used as biopesticide in future


2015 ◽  
Vol 82 (1) ◽  
pp. 268-278 ◽  
Author(s):  
Yannick Raoul des Essarts ◽  
Jérémy Cigna ◽  
Angélique Quêtu-Laurent ◽  
Aline Caron ◽  
Euphrasie Munier ◽  
...  

ABSTRACTDevelopment of protection tools targetingDickeyaspecies is an important issue in the potato production. Here, we present the identification and the characterization of novel biocontrol agents. Successive screenings of 10,000 bacterial isolates led us to retain 58 strains that exhibited growth inhibition properties against severalDickeyasp. and/orPectobacteriumsp. pathogens. Most of them belonged to thePseudomonasandBacillusgenera.In vitroassays revealed a fitness decrease of the testedDickeyasp. andPectobacteriumsp. pathogens in the presence of the biocontrol agents. In addition, four independent greenhouse assays performed to evaluate the biocontrol bacteria effect on potato plants artificially contaminated withDickeya dianthicolarevealed that a mix of three biocontrol agents, namely,Pseudomonas putidaPA14H7 andPseudomonas fluorescensPA3G8 and PA4C2, repeatedly decreased the severity of blackleg symptoms as well as the transmission ofD. dianthicolato the tuber progeny. This work highlights the use of a combination of biocontrol strains as a potential strategy to limit the soft rot and blackleg diseases caused byD. dianthicolaon potato plants and tubers.


PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0179245 ◽  
Author(s):  
Roja Rani Pallavali ◽  
Vijaya Lakshmi Degati ◽  
Dakshayani Lomada ◽  
Madhava C. Reddy ◽  
Vijaya Raghava Prasad Durbaka

Nematology ◽  
2017 ◽  
Vol 19 (3) ◽  
pp. 271-280 ◽  
Author(s):  
Julio C.P. Silva ◽  
Vicente P. Campos ◽  
Eduardo S. Freire ◽  
Willian C. Terra ◽  
Liliana E. Lopez

Ethanol (EtOH) is less harmful to humans than currently available nematicide molecules. This study evaluated the efficacy of EtOH in controlling Meloidogyne incognita in vitro and in lettuce plants under glasshouse conditions. Aqueous EtOH solutions (5-70% volume) and their vapours caused an acute nematicidal effect in vitro in second-stage juveniles (J2) of M. incognita and reduced hatching of J2. There was a large reduction of galls and eggs in the root system when 40 ml of EtOH was applied to M. incognita-infested soil at concentrations of 40 and 70%. Water exposed to EtOH vapours for 1 h became toxic, and a 12-h exposure caused 100% J2 mortality. Use of a plastic cover did not increase the efficiency of EtOH in controlling M. incognita in lettuce plants. The observed EtOH effects indicate its prospective use in controlling plant-parasitic nematodes, especially in glasshouses.


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