Characterization of the genetic diversity of the Tall coconut (Cocos nucifera L.) in the Dominican Republic using microsatellite (SSR) markers

2009 ◽  
Vol 6 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Reina Teresa Martinez ◽  
Luc Baudouin ◽  
Angélique Berger ◽  
Michel Dollet
Keyword(s):  
Genetic Diversity ◽  
Dominican Republic ◽  
Ssr Markers ◽  
Cocos Nucifera ◽  
Cocos Nucifera L

Development of EST-SSR markers for genetic diversity analysis in coconut (Cocos nucifera L.)

2020 ◽  
Vol 47 (12) ◽  
pp. 9385-9397
Author(s):  
P. Preethi ◽  
Shafeeq Rahman ◽  
S. Naganeeswaran ◽  
A. A. Sabana ◽  
K. P. Gangaraj ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Cocos Nucifera ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Cocos Nucifera L

Isolation and characterization of polymorphic microsatellites in Cocos nucifera L.

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 668-675 ◽  
Author(s):  
R Rivera ◽  
K J Edwards ◽  
JHA Barker ◽  
G M Arnold ◽  
G Ayad ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genomic Library ◽  
Cocos Nucifera ◽  
Jaccard Coefficient ◽  
High Frequencies ◽  
Isolation And Characterization ◽  
Analysis Of Similarity ◽  
Cocos Nucifera L ◽  
Shared Alleles

Microsatellites or simple sequence repeats (SSRs) were isolated from coconut (Cocos nucifera) and tested for polymorphism on restricted germplasm. Sequencing of 197 clones from a cv. Tagnanan Tall-enriched genomic library showed that 75% contained a microsatellite, of which 64% were dinucleotide (GA/CT, CA/GT and GC/CG), 6% were trinucleotide, and 30% were compound repeats. Of 41 primer pairs tested on Tagnanan Tall genomic DNA, 38 gave the expected size product, two amplified two loci, and another gave a multilocus pattern. On 20 coconut samples, the 38 SSRs detected 198 alleles (average: 5.2 alleles per microsatellite). Genetic diversity (D = 1 - Sigma pi2) values ranged from 0.141 to 0.809. Heterozygotes were present at high frequencies among some dwarf samples. Analysis of similarity matrices based either on shared alleles at each locus (simple matching coefficient) or on allele bands across all loci (Jaccard coefficient) showed similar results. Dwarfs grouped separately from talls and showed less genetic diversity. In a wider test on 40 samples, 8 SSRs detected 64 alleles (average: eight alleles per microsatellite). These results indicate the high potential of microsatellites to detect genetic diversity in coconut germplasm.Key words: molecular markers, microsatellite, SSR, Cocos nucifera, coconut.

scholarly journals Genetic Diversity Evaluation of MARDI’s Coconut (Cocos nucifera L.) Germplasm using Simple Sequence Repeats

CORD ◽  
2016 ◽  
Vol 32 (2) ◽  
pp. 9
Author(s):  
H.N. Khairun
Keyword(s):  
Ssr Markers ◽  
Cocos Nucifera ◽  
Germplasm Collection ◽  
West African ◽  
Average Value ◽  
New Information ◽  
Ssr Loci ◽  
Cocos Nucifera L ◽  
Simple Sequence

A total of 18 simple sequence repeat (SSR) markers have been optimized and used to genotype coconut (Cocos nucifera L.). These markers were used to genotype 23 coconut varieties that were maintained in MARDI’s germplasm collection. Fifteen SSR loci were polymorphic markers while the remaining three SSR were monomorphic.  The number of alleles ranged from 2 to 19 with a mean number of 8.53 per locus. The expected heterozygosity values in each variety ranged from 0.07 to 0.61, with an average value of 0.52. Several varieties could be successfully differentiated by using these 15 SSR markers such as Cameroon Red Dwarf x West African Tall hybrid, Mawa hybrid, Malayan Tall x Cameroon Red Dwarf hybrid, Malayan Red Dwarf x Rotuman hybrid, Catigan, Pandan and Laguna. A UPGMA Dendogram showed Niu Damu as an outlying group with high dissimilarity from all other varieties. Analyses using the STRUCTURE software showed all 23 varieties to be clustered into 21 genotypic groups. This new information will greatly contribute towards characterization of the MARDI’s coconut germplasm collection and to develop a SSR tool for the identification of new coconut varieties in Malaysia.

Characterization of GA20ox genes in tall and dwarf types coconut (Cocos nucifera L.)

2018 ◽  
Vol 40 (7) ◽  
pp. 735-745
Author(s):  
Tippawan Boonkaew ◽  
Chareerat Mongkolsiriwatana ◽  
Ananya Vongvanrungruang ◽  
Kornsorn Srikulnath ◽  
Surin Peyachoknagul
Keyword(s):  
Cocos Nucifera ◽  
Cocos Nucifera L

Extraction and characterization of cellulose microfibrils from agricultural residue – Cocos nucifera L.

2012 ◽  
Vol 46 ◽  
pp. 555-563 ◽  
Author(s):  
C. Uma Maheswari ◽  
K. Obi Reddy ◽  
E. Muzenda ◽  
B.R. Guduri ◽  
A. Varada Rajulu
Keyword(s):  
Cocos Nucifera ◽  
Cellulose Microfibrils ◽  
Agricultural Residue ◽  
Cocos Nucifera L

Molecular characterization of almond accessions from the island of La Palma (Canary Islands, Spain) using SSR markers

2014 ◽  
Vol 12 (3) ◽  
pp. 323-329 ◽  
Author(s):  
Guillermo Padilla ◽  
Rafel Socias i Company ◽  
Amando Ordás
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Average Value ◽  
La Palma ◽  
Expected Heterozygosity ◽  
Commercial Cultivars ◽  
Soft Shell ◽  
Genetic Profiles ◽  
Simple Sequence

In this study, 15 simple sequence repeat (SSR) markers were used for genetic diversity analysis of 45 almond accessions, which included 25 local cultivars from La Palma Island and three other commercial cultivars. A total of 110 amplification fragments were produced, with an average value of 7.9 alleles per locus. Twelve of the SSR markers can be considered as highly informative, with values of expected heterozygosity and power of discrimination above 0.5 and 0.8, respectively. Due to cases of synonymy and homonymy, 37 different genetic profiles were obtained, with the homonymy of the soft-shell varieties known as ‘Mollar’ being the most significant. Cluster analysis identified four groups within the accessions. One of these groups exclusively consisted of the two commercial cultivars ‘Guara’ and ‘Ferraduel’. The other commercial cultivar used in the study, ‘Desmayo Largueta’, was in a cluster with three cultivars from the same locality. The analysis of molecular variance revealed that the within-localities component accounts for most of the total variation, suggesting that La Palma almond cultivars did not originate independently in different parts of the island. The results of the study reveal the genetic singularity of La Palma almond cultivars and the genetic diversity among them.

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